کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9754407 | 1494678 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of daunomycin antibiotic with human serum albumin: Investigation by resonant mirror biosensor technique, fluorescence spectroscopy and molecular modeling methods
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Interaction of daunomycin antibiotic with human serum albumin: Investigation by resonant mirror biosensor technique, fluorescence spectroscopy and molecular modeling methods Interaction of daunomycin antibiotic with human serum albumin: Investigation by resonant mirror biosensor technique, fluorescence spectroscopy and molecular modeling methods](/preview/png/9754407.png)
چکیده انگلیسی
Daunomycin (DM) is a clinically used antitumor anthracycline antibiotic, which is transported primarily by human serum albumin (HSA) in the blood. Binding characteristics are therefore of interest for both the pharmacokinetics and pharmacodynamics of DM. A new optical biosensor technique based on the resonant mirror was used to characterize interaction of DM with HSA at different temperatures and the affinity constants were obtained. The HSA-DM interaction is exothermic with having favorable enthalpy and entropy followed by the integrated van't Hoff equation analysis. Fluorescence studies showed that DM has an ability to quench the intrinsic fluorescence of HSA through a static quenching procedure according to the Stern-Volmer equation and DM displays a pH-dependent binding affinity to HSA. Molecular modeling calculations showed that the DM binds HSA to a non-classical drug binding site and further analysis of the binding site of DM within the HSA molecule suggested that hydrophobic contacts, hydrogen bond formation and electrostatic interactions account for the binding of DM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 3â4, 15 September 2005, Pages 404-410
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 39, Issues 3â4, 15 September 2005, Pages 404-410
نویسندگان
Kai Tang, Yi-Min Qin, Ai-Hua Lin, Xing Hu, Guo-Lin Zou,