کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9757472 | 1496004 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A study of cytokinetic and motile prostate cancer cells using synchrotron-based FTIR microspectroscopic imaging
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A study of cytokinetic and motile prostate cancer cells using synchrotron-based FTIR microspectroscopic imaging A study of cytokinetic and motile prostate cancer cells using synchrotron-based FTIR microspectroscopic imaging](/preview/png/9757472.png)
چکیده انگلیسی
Synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy has been applied to the study of dynamic cellular events. SR-FTIR microspectroscopy is a powerful bioanalytical technique for the simultaneous analysis of proteins, lipids and a variety of phosphorylated molecules within whole cells. In this study, SR-FTIR microspectroscopy was used in imaging mode to generate biospectroscopic chemical maps of formalin-fixed PC-3 prostate cancer cells, which had been preserved in the process of cell division (cytokinesis) and locomotion. The distribution and intensity profiles of IR signals corresponding to the amide I and II (protein) and/or νas and s (CH3), νas and s (CH2) (lipid) modes were found to be useful in understanding fundamental biochemical processes at the midbody of the cytokinetic cells and at the lamellipodium of motile cells. Furthermore, in both of these cellular systems we observed a distinct hemispherical distribution of the lipid ester (CO) signal, which surrounded high phosphate signal. This feature was assigned to intracellular IR signals corresponding to membrane-rich organelles surrounding the cell nucleus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 193-201
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 193-201
نویسندگان
E. Gazi, J. Dwyer, N.P. Lockyer, J. Miyan, P. Gardner, C.A. Hart, M.D. Brown, N.W. Clarke,