کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9770458 | 1501949 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amphipathic cyclooctapeptides: interactions with detergent micelles and metal ions
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to study the secondary structural preferences of amphipathic cyclopeptides in detergent assemblies and their interactions with metal ions, two basic amphiphatic cyclooctapeptides, c[(Lys-d-Lys)2-Xaa-d-Leu-Leu-d-Leu], where Xaa is Leu (P1) or Trp (P2), were studied by circular dichroism (CD) spectroscopy. In water, P1 exhibited an unordered secondary structure whereas P2 adopted a partial β-sheet structure. Temperature effect on the CD of these cyclopeptides showed small changes over the temperature range from 278 to 353 K. P1 showed low and non-specific binding affinity for metal ions (Ca2+, Zn2+, Na+, or Li+) in water whereas P2 did not exhibit any significant interaction with these ions. However, in the zwitterionic micellar detergent dodecylphosphocholine (DPC), P2 adopted a β-sheet structure, which exhibited a greater propensity for metal ion interactions (K1â¼103 Mâ1 and K2â¼102 Mâ1). Variable temperature CD studies on the peptide-metal ion complexes showed that these interactions are thermally reversible. Our results indicate that amphipathic cyclooctapeptides can co-assemble with micellar DPC and are capable of interacting with metal ions. This study will improve our ability to design a better metal ion sensing cyclopeptide in co-micellar assembles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 733, Issues 1â3, 3 January 2005, Pages 5-11
Journal: Journal of Molecular Structure - Volume 733, Issues 1â3, 3 January 2005, Pages 5-11
نویسندگان
William Day Gates, Jack Rostas, Bobby Kakati, Maria Ngu-Schwemlein,