کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9882062 1536535 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanism of interaction of PITPα with membranes: Conformational changes in the C-terminus associated with membrane binding
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mechanism of interaction of PITPα with membranes: Conformational changes in the C-terminus associated with membrane binding
چکیده انگلیسی
Eukaryotic phosphatidylinositol transfer proteins (PITPs) are composed predominantly of small (∼32 kDa) soluble proteins that bind and transfer a single phospholipid, normally phosphatidylinositol or phosphatidycholine. Two forms, PITPα and PITPβ, which share approximately 80% amino acid sequence similarity, are known. Rat PITPα was labeled at specific single reactive Cys residues with I-AEDANS and used to examine PITP-membrane interactions. Upon binding to phospholipid vesicles, PITP labeled with AEDANS at the C-terminus, a region postulated to be involved in membrane binding, shows significant decreases in both steady-state and dynamic fluorescence anisotropy. In contrast, PITPs labeled with AEDANS at sites located distal to the C-terminus show increases in both steady-state and dynamic anisotropy. These results suggest that interaction of PITP with membrane surfaces leads to significant alterations in conformation and perhaps melting of the C-terminal helix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 444, Issue 2, 15 December 2005, Pages 112-120
نویسندگان
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