کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9882156 | 1536543 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Correlation between bovine calpastatin mRNA transcripts and protein isoforms
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Calpastatin is a specific calpain protease inhibitor: calpains are a family of calcium-activated neutral proteases, which have been implicated in various processes. Despite all the available data concerning calpastatin, little is known about how this gene is regulated, particularly in bovine. The existence of four types of transcripts differing at their 5â² ends (Type I, II, III, and IV) has been demonstrated. Here, we show that the Type I, II, and III transcripts are ubiquitous while Type IV is testis-specific. In addition, a Northern blot analysis revealed that the Type III transcript may have three different 3â² termini. Using specific anti-peptide anti-sera, a correspondence between a 145 and a 125Â kDa isoforms, and Type I and/or II and III transcripts, respectively, has been established. Finally, we discuss the origin of a 70Â kDa isoform, recognized by anti-sera directed against the N-terminal region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 440, Issue 1, 1 August 2005, Pages 46-53
Journal: Archives of Biochemistry and Biophysics - Volume 440, Issue 1, 1 August 2005, Pages 46-53
نویسندگان
Peggy Raynaud, Mélanie Gillard, Tim Parr, Ronald Bardsley, Valérie Amarger, Hubert Levéziel,