کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9884434 | 1536794 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
A new gene (GenBank Accession No. AF114264) was cloned from umbilical vein wall tissue by using RT-PCR. The gene shares high similarity to the gene encoding F-actin binding protein nexilin, so named as NELIN. A clone of 2737Â bp contains open reading frame of 1344Â bp extending from 412 to 1755. NELIN was expressed primarily in the heart and skeletal muscle among eight tested normal tissues. Immunofluorescence and immunoprecipitation demonstrated that NELIN product was associated with F-actin. Stable transfection of NELIN into HeLa cells increased the cell migration by 2.17-fold and the adhesion by 1.67-fold, respectively, compared to cells with the empty vector (PÂ <Â 0.05). The results support that NELIN product is an F-actin associated protein and mediates cell motility.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 330, Issue 4, 20 May 2005, Pages 1127-1131
Journal: Biochemical and Biophysical Research Communications - Volume 330, Issue 4, 20 May 2005, Pages 1127-1131
نویسندگان
Wei Wang, Weili Zhang, Yu Han, Jingzhou Chen, Yibo Wang, Zhen Zhang, Rutai Hui,