کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9884455 | 1536794 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Macrophage colony-stimulating factor expression in retrovirally transduced cells is dependent upon both the adherence status of the target cells and its 5â² flanking untranslated region
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Numerous cell types retrovirally transduced with macrophage colony-stimulating factor (M-CSF) using LXSN-based vectors showed a variable expression of the transgene. Expression of M-CSF correlated with the cells' adherent status. Transduced adherent cells produced the M-CSF, whereas the non-adherent cells synthesized little M-CSF. Studies showed that the 5â²-UTR of the M-CSF gene regulated transgenic M-CSF gene expression. Ligation of this 5â²-UTR to the enhanced green fluorescent protein gene (EGFP) caused the expression of EGFP to show the same dichotomy as previously seen with the M-CSF. Transgenic M-CSF was expressed within non-adherent cells when the 5â²-UTR was removed from the LXSN vector. Quantitative real-time polymerase chain reaction analysis confirmed that lesser production of M-CSF mRNA occurred within the non-adherent cells than in the adherent cells. This difference was eliminated when the 5â²-UTR was removed from the retroviral vector. Our work suggests that this 5â²-UTR of the M-CSF gene could be an important way to get transgenic expression within adherent cells, but not in non-adherent cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 330, Issue 4, 20 May 2005, Pages 1275-1284
Journal: Biochemical and Biophysical Research Communications - Volume 330, Issue 4, 20 May 2005, Pages 1275-1284
نویسندگان
Jian-Gang Zhang, Qinghong Dan, Timothy C. Fong, Christopher C. Williams, Maria D. Avina, Mehrdokht Tarbiyat-Boldaji, Sakineh Khalaghizadeh, Michael Irwin, Amy Nguyen, Jing-Li Zhuang, Neil Hoa, H. Terry Wepsic, Martin R. Jadus,