کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9884685 | 1537058 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proton pumping by complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica reconstituted into proteoliposomes
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کلمات کلیدی
Yarrowia lipolyticaFCCPTX-100DBQn-octyl-β-d-glucopyranosideAcmA2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinonen-decylubiquinonehexaammineruthenium(III)-chlorideETH 1579-amino-6-chloro-2-methoxyacridine - 9-آمینو-6-کلرو-2-متکی آکرییدینHAR - اندReconstitution - بازسازیTriton X-100 - تریتون X-100 Complex I - مجتمع IMitochondria - میتوکندریا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The mechanism of energy converting NADH:ubiquinone oxidoreductase (complex I) is still unknown. A current controversy centers around the question whether electron transport of complex I is always linked to vectorial proton translocation or whether in some organisms the enzyme pumps sodium ions instead. To develop better experimental tools to elucidate its mechanism, we have reconstituted the affinity purified enzyme into proteoliposomes and monitored the generation of ÎpH and ÎÏ. We tested several detergents to solubilize the asolectin used for liposome formation. Tightly coupled proteoliposomes containing highly active complex I were obtained by detergent removal with BioBeads after total solubilization of the phospholipids with n-octyl-β-d-glucopyranoside. We have used dyes to monitor the formation of the two components of the proton motive force,ÎpH and ÎÏ, across the liposomal membrane, and analyzed the effects of inhibitors, uncouplers and ionophores on this process. We show that electron transfer of complex I of the lower eukaryote Y. lipolytica is clearly linked to proton translocation. While this study was not specifically designed to demonstrate possible additional sodium translocating properties of complex I, we did not find indications for primary or secondary Na+ translocation by Y. lipolytica complex I.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1710, Issues 2â3, 20 December 2005, Pages 87-95
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1710, Issues 2â3, 20 December 2005, Pages 87-95
نویسندگان
Stefan Dröse, Alexander Galkin, Ulrich Brandt,