کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9884712 1537060 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The yeast cAMP protein kinase Tpk3p is involved in the regulation of mitochondrial enzymatic content during growth
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The yeast cAMP protein kinase Tpk3p is involved in the regulation of mitochondrial enzymatic content during growth
چکیده انگلیسی
During aerobic cell growth, mitochondria must meet energy demand either by adjusting cellular mitochondrial content or by adjusting ATP production flux, allowing a constant growth yield. On respiratory substrate, the Ras/cAMP pathway has been shown to be involved in this process in the yeast Saccharomyces cerevisiae. We show that of the three cAMP protein kinase catalytic subunits, Tpk3p is the one specifically involved in the regulation of cellular mitochondrial content when energy demand decreases. In decreased energy demand, the Δtpk3 mitochondrial enzymatic content decreases leading to a subsequent decrease in the cellular growth rate. Moreover, enzymatic content decreases in the Δtpk3 isolated mitochondria, suggesting that the amount of cellular mitochondria is not affected, but rather that the mitochondria are modified. Our study points to an important decrease in the cytochrome c content in the Δtpk3 mitochondria, which leads to a decrease in the slipping process at the level of cytochrome-c-oxidase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1706, Issues 1–2, 7 January 2005, Pages 117-125
نویسندگان
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