کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885530 | 1537327 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tilapia metallothionein genes: PCR-cloning and gene expression studies
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کلمات کلیدی
nuclear factor-interleukin 6Tris-Borate-EDTA bufferIPTGGRENF-IL6KBPTBEAP1MREisopropylthio-β-d-galactoside - isopropylthio-β-d galactosideSp1 - SP1Biomarker of exposure - بیومارکر از قرار گرفتن در معرضabsorbance (1 cm) - جذب (1 سانتیمتر)reverse transcription - رونویسی معکوسGene transcription - رونویسی ژنGlucocorticoid response element - عنصر پاسخ گلوکوکورتیکوئیدHeavy metals - فلزات سنگینMetallothionein - متالوتونیئینunit - واحدpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازbasepair(s) - پایه (های)Specific protein 1 - پروتئین خاص 1activator protein 1 - پروتئین فعال کننده 1kilobase(s) - کیلو بایت (ها)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Genomic PCR reactions were performed to isolate gene sequences of tilapia metallothionein (tiMT) from Oreochromis mossambicus and Oreochromis aureus. Two AP1 binding sites, four metal responsive elements, and a TATA box are the major cis-acting elements identified in the 800-bp 5â² flanking region of the tiMTs obtained in this study. The tiMT gene promoter cloned from O. aureus was characterized in vitro using PLHC-1 cell-line, a hepatocellular carcinoma of a desert topminnow (Poecciliopsis lucida), following the administrations of Cd2+, Co2+, Cu2+, Ni2+, Pb2+ and Zn2+. Only Cd2+, Pb2+ and Zn2+ were able to induce the transcription of tiMT gene promoter in PLHC-1 cells in a dose-dependent manner. Zn2+ had the highest fold induction of tiMT gene promoter activity. Deletion mutants were tested for their abilities to drive the transcription of reporter gene following Cd2+ and Zn2+ administrations. However, Cu2+ and Ni2+ also induced the production of hepatic MT mRNA in vivo. Northern blot analysis showed that liver gave the highest fold induction of MT gene expression following the administration of heavy metal ions. These data indicated that hepatic MT mRNA level in tilapia is a potential sensitive biomarker of exposure to various metal ions including Cu2+, Cd2+, Ni2+, Pb2+, Hg2+ and Zn2+ ions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1731, Issue 3, 20 December 2005, Pages 191-201
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1731, Issue 3, 20 December 2005, Pages 191-201
نویسندگان
Cheung Cheung, Andrew Pok Lap, Vincent Kwok Lim Lam, King Ming Chan,