کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885533 | 1537327 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulatory elements of microfibril-associated glycoprotein-1 gene expression in muscle cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
The Mfap2 gene encodes the microfibril-associated glycoprotein-1 (MAGP1), an extracellular matrix protein of microfibrillar structures. The gene is transcribed from a major transcription start site embedded in a CpG island. Mapping of transcriptionally active regions in the 5â² flanking sequence identified a region, located between nucleotides â339 and â109 as the Mfap2 basal promoter. Site-directed and random mutagenesis demonstrated that a KLF sequence motif at â256/â270, an E-box at â222/â229, and a GC-box at â117/â125, are critical for the promoter function. Using electrophoresis mobility shift assays, we find that the KLF motif mediates the binding of GKLF/KLF4, whereas the E-box is a target for both Upstream Stimulatory Factors 1 and 2, and the GC box at â117/â125 forms complexes with Sp1 and Sp3, but not with Sp4 or AP2α. A sequence element spanning position â150 may represent the binding motif of an uncharacterized transcription factor. The basal transcriptional regulation of Mfap2 in muscle cells is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1731, Issue 3, 20 December 2005, Pages 215-224
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1731, Issue 3, 20 December 2005, Pages 215-224
نویسندگان
Fernando Segade, Robert P. Mecham,