کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9885565 | 1537330 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation, sequencing, and functional analysis of the TATA-less human ATPase II promoter
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isolation, sequencing, and functional analysis of the TATA-less human ATPase II promoter Isolation, sequencing, and functional analysis of the TATA-less human ATPase II promoter](/preview/png/9885565.png)
چکیده انگلیسی
Multiple lines of evidence indicate that the P-type Mg2+-ATPase, termed ATPase II, could play an important role in apoptosis. With the long-term objective of studying the regulation of this protein during apoptosis, we delineated the exon-intron organization of the human ATPase II gene (within chromosome 4). Subsequently, we used RNA ligase-mediated rapid amplification of cDNA ends to identify a major transcription start site at position â143 with respect to the translation start site. Luciferase reporter analysis of a 1.2-kb 5â²-flanking sequence (â1222 to +94 with respect to the transcription start site) revealed strong promoter activity in three human cell lines, human oligodendroglioma (HOG), SHSY5Y (hybrid neuroblastoma), and EA.hy926 (endothelial cell line). Serial deletions from the 5â² end of this sequence up to nucleotide â291 yielded some decrease in activity only in the EA.hy926 cells. Further deletion to â217 caused a drastic decrease in activity in all three cell lines, but a â148 fragment showed preferential reduction in activity in the EA.hy926 cells. The promoter activity was nearly equal in two sequence variants of the promoter, one of which (designated as Variant 2) contained a 15-bp direct repeat within a GC-rich region. Additionally, there were several single base-pair changes from the sequence reported by the human genome project. Despite the presence of enhancer/repressor elements, such as Sp1 and NFκB, relatively small differences in promoter activity were observed in the three cell lines. However, it is likely that such sequence elements could cause major regulation of promoter activity in cells subjected to conditions that trigger apoptosis. The ATPase II promoter sequence will provide valuable clues to the regulation and role of the ATPase II protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1728, Issue 3, 1 May 2005, Pages 186-198
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1728, Issue 3, 1 May 2005, Pages 186-198
نویسندگان
Tomasz Sobocki, Farah Jayman, Malgorzata B. Sobocka, Ruth Duchatellier, Probal Banerjee,