کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886477 | 1537827 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source](/preview/png/9886477.png)
چکیده انگلیسی
Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of co-localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex. Rat cerebellum was fixed, freeze-protected by sucrose, frozen and sectioned by cryoultramicrotomy and dried at room temperature. The samples were analyzed in an imaging TOF-SIMS instrument equipped with a Bi1-7+-source. The cholesterol signal (m/z 369 and 385) was localized in Purkinje cells and in nuclei of granular layer cells. The phosphocholine headgroup of phosphatidylcholine and sphingomyelin was localized by imaging a specific fragment (m/z 86). This signal was localized in the molecular layer of cerebellar cortex, in Purkinje cells and in parts of the granular layer probably representing the synapse-rich glomeruli. The galactosylceramide was localized by imaging the quasi-molecular ions at m/z 835 and 851, showed a clear colocalization with cholesterol, but also a specific localization in dots (diameter â¤700 nm) in the molecular layer in the vicinity of Purkinje cells, at Purkinje cells and at cells in the granular layer. The results show a heterogenous distribution of lipids between different cell types not previously described. In order to avoid redistribution artefacts, controls were made by a technique, based on high pressure freezing ,freeze fracturing and freeze drying of samples which were then analyzed by bombardment with a Bi3+ liquid metal ion gun. The galactosylceramide and cholesterol were found distributed as spots in the granular layer. The spots were of homogeneous size with a diameter of <700 nm. Although the galactosylceramide and cholesterol were localized to the same areas, there were clear differences in their distribution at higher resolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1737, Issues 2â3, 15 December 2005, Pages 102-110
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1737, Issues 2â3, 15 December 2005, Pages 102-110
نویسندگان
HÃ¥kan Nygren, Katrin Börner, Birgit Hagenhoff, Per Malmberg, Jan-Eric MÃ¥nsson,