کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886626 | 1537840 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oxidized but not acetylated low-density lipoprotein reduces preproinsulin mRNA expression and secretion of insulin from HIT-T15 cells
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کلمات کلیدی
AcLDLnLDLHIT-T15 cellKRBBoxLDLFBSBSA - BSAAcetylated LDL - LDL آستیلت شدهOxidized LDL - LDL اکسید شدهROS - ROSbovine serum albumin - آلبومین سرم گاوKrebs–Ringer bicarbonate buffer - بافر کربنات کربن-رینگرEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاOxidative stress - تنش اکسیداتیوfetal bovine serum - سرم جنین گاوPancreatic beta cell - سلول بتای پانکراسOxidized low-density lipoprotein - لیپوپروتئین با چگالی کم اکسید شدهLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)Lipid peroxidation - پراکسیداسیون لیپیدReactive oxygen species - گونههای فعال اکسیژنScavenger receptor - گیرنده مخرب
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Oxidized but not acetylated low-density lipoprotein reduces preproinsulin mRNA expression and secretion of insulin from HIT-T15 cells Oxidized but not acetylated low-density lipoprotein reduces preproinsulin mRNA expression and secretion of insulin from HIT-T15 cells](/preview/png/9886626.png)
چکیده انگلیسی
We examined the effect of oxidized low-density lipoprotein (oxLDL) on the insulin secretion in the culture of HIT-T15 cell line, an islet β-cell line derived from a hamster pancreatic tumor. In order to check the uptake of modified LDL by HIT-T15 cells, we prepared DiI-labeled native LDL (nLDL), acetylated LDL (AcLDL), and oxLDL. After the addition of each LDL into the cultures of HIT-T15 cells, fluorescence microscopic study was done. It was suggested that AcLDL and oxLDL were taken up by HIT-T15 cells, as well as nLDL. mRNA expression of the LDL receptor, CD36, and SR-B1 was detected in HIT-T15 by RT-PCR. The medium insulin level was measured in the culture of HIT-T15 cells with each LDL. oxLDL significantly reduced the insulin secretion stimulated by various concentrations of glucose, the intracellular content of insulin, and the expression of preproinsulin mRNA compared to the control cultures without LDL addition. In contrast, nLDL and AcLDL had no effect on the insulin secretion, the intracellular insulin level, or the expression of preproinsulin mRNA. MTT assay findings (reflecting cell numbers) were not different between cultures with and without LDLs. These results indicated that oxLDL disturbed the insulin metabolism of HIT-T15 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1687, Issues 1â3, 21 February 2005, Pages 173-180
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1687, Issues 1â3, 21 February 2005, Pages 173-180
نویسندگان
Fumitaka Okajima, Mikiko Kurihara, Chihaya Ono, Yasushi Nakajima, Kyoko Tanimura, Hitoshi Sugihara, Atsushi Tatsuguchi, Kiyotaka Nakagawa, Teruo Miyazawa, Shinichi Oikawa,