کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9889958 | 1539994 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Discrimination between two steps in the mitochondrial permeability transition process
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کلمات کلیدی
PhAsOCypDMOPSCyclophilin DBCECFVDACΔΨmpyrGFCCPDEPCMPTANTNH2OHHematoporphyrin IXEGTAPBR4-morpholinepropanesulfonic acid - 4-مورفولین پروپان سولفونیک اسیدPermeability transition - انتقال نفوذپذیریmitochondrial permeability transition - انتقال نفوذپذیری میتوکندریPhenylarsine oxide - اکسید فنیلارسینCSA - ایالات مؤتلفهٔ آمریکاFluorescence anisotropy - بی خوابی فلورسانسیadenine nucleotide translocase - ترانسکوز نوکلئوتید آدنینHIS - خودDiamide - دیامیدdiethyl pyrocarbonate - دیورتیک پریکو کربناتDIA - روزLag time - زمان لاغرcyclosporin A - سیکلوسپورین AMitochondria - میتوکندریاHematoporphyrin - هماتوپورفیرینHydroxylamine - هیدروکسیلامینhistidine - هیستیدینMembrane potential - پتانسیل غشاء voltage-dependent anion channel - کانال آنیون وابسته به ولتاژ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
It is well known that a lag phase generally elapses between the addition of inducers of the mitochondrial permeability transition and the opening of the pore. To advance our present understanding as regards the significance of this phenomenon, we used experimental approaches which are sensitive to different aspects of the permeability transition process. The pore conformation was sensed by the fluorescence anisotropy changes of hematoporphyrin-labelled mitochondria. Membrane permeabilization was ascertained by following the matrix swelling consequent to external solute equilibration. We show that the anisotropy changes of mitochondria-bound hematoporphyrin precede both membrane depolarization (proton permeation) and matrix swelling (solute permeation), thus sensing a step of the permeability transition process that involves the pore in its closed state. We suggest that the opening of the pore is preceded by a structural remodelling of mitochondrial domains containing hematoporphyrin-near, pore-regulating histidines. Such a perturbation is strongly inhibited at acidic matrix pH and completely blocked by cyclosporin A. In sucrose-based media the opening of the pore can be strongly delayed, as compared to salt-based media, a fact which probably reflects perturbation of mitochondrial membranes by sugar. We conclude that the mitochondrial permeability transition could be described as an at least two-step process which is mainly regulated by conformational changes of the pore components.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 9, September 2005, Pages 1858-1868
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 9, September 2005, Pages 1858-1868
نویسندگان
Fernanda Ricchelli, Giulio Jori, Silvano Gobbo, Peter Nikolov, Valeria Petronilli,