کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9890129 | 1540002 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
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کلمات کلیدی
GAPDHHGFMAGPsiRNA - siRNAElastogenesis - الاستوژنزsmall interference RNA - تداخل کوچک RNATropoelastin - تروپلوستینminimum essential medium - حداقل حداقل مورد نیازHuman gingival fibroblasts - فیبروبلاستهای لثه انسانی انسانیFibrillin - فیبریلینMEM - مامانMicrofibril-associated glycoprotein - گلیکوپروتئین مرتبط با میکرو فیبریل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro](/preview/png/9890129.png)
چکیده انگلیسی
Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Microfibril-associated glycoprotein-1 (MAGP-1) and fibrillin-2, the major components of microfibrils, provide the likely template for tropoelastin deposition. In this study, we used the RNA interference (RNAi) technique to establish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in elastin-producing cells (human gingival fibroblasts). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to <30% of the control level, and this was responsible for the diminution of tropoelastin deposition. An immunofluorescence study also confirmed that RNAi-mediated down-regulation of MAGP-1 or fibrillin-2 led to the loss of tropoelastin immunoreactivity. These results suggest that MAGP-1 and fibrillin-2 are, directly or indirectly, associated with the extracellular deposition of tropoelastin during elastic fiber formation in human gingival fibroblasts in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 1, January 2005, Pages 120-129
Journal: The International Journal of Biochemistry & Cell Biology - Volume 37, Issue 1, January 2005, Pages 120-129
نویسندگان
Eichi Tsuruga, Toshihiko Yajima, Kazuharu Irie,