کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9891520 | 1540764 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibitory effects of glycolipids fraction from spinach on mammalian DNA polymerase activity and human cancer cell proliferation
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کلمات کلیدی
DGDGMGDGTLCSQDGDTTP2′-deoxythymidine 5′-triphosphateDNA polymerase α - DNA پلیمراز aSpinach - اسفناجdigalactosyl diacylglycerol - دیگالاکتوزیل دیسیل گلیسرولSulfoquinovosyl diacylglycerol - سولفووینوواسییل دیسیل گلیسرولAnticancer agent - عامل ضد سرطانEnzyme inhibitor - مهارکننده آنزیمMonogalactosyl diacylglycerol - مونوگلاکتوزیل دیسیlglycerolpol - پلthin-layer chromatography - کروماتوگرافی نازک لایهGlycolipids - گلیکوالیپید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We succeeded in purifying the fraction containing the major glycolipids in monogalactosyl diacylglycerol, digalactosyl diacylglycerol and sulfoquinovosyl diacylglycerol (SQDG) from dried vegetables. This glycolipids fraction was an inhibitor of DNA polymerase α (pol α) in vitro and also the proliferation of human cancer cells. In this study, eight common vegetables were investigated in terms of the glycolipids fraction, the amounts of major glycolipids, mammalian DNA polymerase inhibitory activity and antiproliferative activity toward human cancer cells. Green tea possessed the largest amount of glycolipids overall. Spinach contained the largest amount of SQDG, followed by parsley, green onion, chive, sweet pepper, green tea, carrot and garlic. Spinach had the strongest inhibitory effect on pol α activity and human cancer cell proliferation. A significant correlation was found between SQDG content and inhibition of DNA polymerase. Therefore, the inhibition of pol α activity by SQDG may lead to cell growth suppression. Of the six subspecies of spinach (Spinacia oleracea) tested, “Anna” had the largest amount of SQDG, strongest inhibitory activity toward DNA polymerase and greatest effect on human cancer cell proliferation. Based on these results, the glycolipids fraction from spinach is potentially a source of food material for a novel anticancer activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 16, Issue 10, October 2005, Pages 594-601
Journal: The Journal of Nutritional Biochemistry - Volume 16, Issue 10, October 2005, Pages 594-601
نویسندگان
Isoko Kuriyama, Keiichi Musumi, Yuko Yonezawa, Masaharu Takemura, Naoki Maeda, Hiroshi Iijima, Takahiko Hada, Hiromi Yoshida, Yoshiyuki Mizushina,