کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9893134 | 1541305 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation
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کلمات کلیدی
PBSMSCsHO-1Heme oxygenase-1Heme Oxygenase-1 (HO-1)bone marrow mesenchymal cellsMyocardial infarction - آنفارکتوس میوکاردCSA - ایالات مؤتلفهٔ آمریکاFraction shortening - تقسیم مکررCytoprotection - حفاظت از سیتوMyosin heavy chain - زنجیره سنگین میوزینcyclosporin A - سیکلوسپورین AMHC - مجموعه سازگاری بافتی اصلیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریTransplantation - پیوندejection fraction - کسری خروجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Heme oxygenase-1 (HO-1) plays an important role in oxidative stress and recent studies indicate that it is a graft survival protein in cardiac and liver transplant models. In this study, we investigated the relation between the expressions of HO-1 and the effects of human bone marrow mesenchymal cells (MSCs) transplantation to xenogenic rat hearts with experimental myocardial infarction (MI). A total of 5 à 106 cells in 100 μl PBS or equal volume PBS alone were injected into the ischemic zones immediately post-MI. At 1, 3, and 7 days post-MI, cardiac function was evaluated by echocardiography, the expression of HO-1 was assessed by real-time PCR and Western blot, the localization of HO-1 protein was determined under immunofluorescence microscopy. The infarct size was examined by histology. The numbers of Hoechst-33342 positive MSCs were evaluated under immunofluorescence microscopy and also by flow cytometry after isolation from the host hearts. The results indicated that the HO-1 expressions were markedly increased at both mRNA and protein levels in comparison with injection of PBS at each time point post MSCs transplantation (P < 0.01). HO-1 was revealed both in transplanted MSCs and recipient cardiomyocytes by immunofluorescence. Up-regulated HO-1 expression was accompanied by increase of the numbers of Hoechst-33342 positive MSCs, the reduction of infarct size, and the improvement of cardiac function. Transplantation of human MSCs could up-regulate HO-1 expression in infarct rat hearts, which might play an important role in protecting transplanted MSCs, cardiomyocytes survival, and cardiac function improvement during the early stage after MI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 69, Issues 1â2, January 2005, Pages 64-70
Journal: Microvascular Research - Volume 69, Issues 1â2, January 2005, Pages 64-70
نویسندگان
Shaoheng Zhang, Shun Lu, Junbo Ge, Jingxuan Guo, Ping Chen, Tao Li, Ping Zhang, Zhuqing Jia, Kangtao Ma, Yonggang Liu, Chunyan Zhou, Linsong Li,