کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9894085 | 1542260 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of prostaglandin E1 on ion currents of NG108-15 cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to elucidate the mechanism by which prostaglandin E1 (PGE1) acts on ion currents of whole-cell voltage-clamped NG108-15 neuroblastoma Ã glioma hybrid cells. Ruptured and perforated patch were used. The holding current at â70 mV, the current-voltage curve produced by ramp pulses from â70 to 0 mV and the T-type and hva (high-voltage-activated) Ca2+ currents associated with rectangular pulses were recorded. Bath application of PGE1 (0.2 or 3 μM) reversibly increased the holding current, an effect mimicked by the prostanoid agonist iloprost (5-50 nM). The PGE1 effect was totally blocked by the cAMP-antagonist Rp-cAMPS whereas H-89, an inhibitor of protein kinase A (PKA), failed to inhibit it, even when applied in the fairly high bath concentration of 30 μM. PGE1 and iloprost also inhibited the T-type and hva Ca2+ currents and this effect of PGE1 was likewise not prevented by H-89. In some of the cells, the PGE1 effect on holding current could be mimicked by 8-pCPT-2Me-cAMP (100-300 μM), a selective agonist of Epac (exchange protein activated by cAMP), but unlike the PGE1 effect its action was not abolished by Rp-cAMPS. The effect of PGE1 on the the holding current and on the T-type Ca2+ current was diminished when EGTA in the pipette solution was replaced by BAPTA, suggesting that Ca2+ ions are involved in the PGE1 effect. It is concluded that the PGE1 effect is mediated by cAMP and Ca2+ ions but not by PKA or Epac.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 76, Issues 1â4, May 2005, Pages 117-132
Journal: Prostaglandins & Other Lipid Mediators - Volume 76, Issues 1â4, May 2005, Pages 117-132
نویسندگان
Hans Meves,