کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9902192 1545793 2005 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of one VH antibody sequence in both healthy donors and urticaria patients
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Detection of one VH antibody sequence in both healthy donors and urticaria patients
چکیده انگلیسی
We have previously isolated anti-FcεRIα autoantibodies from phage libraries of healthy donors and urticaria patients. Strikingly, the same antibody, LTMα15, was isolated from both libraries. Sequence analysis revealed a germline configuration of the LTMα15 variable heavy (VH) chain with a slightly mutated variable light (VL) chain supporting its classification as a natural autoantibody. Distribution analysis of anti-FcεRIα autoantibodies by functional or serological tests delivered conflicting data. For this reason we have developed a new real-time PCR to analyse the distribution of LTMα15VH in healthy donors and urticaria patients. Our new bioinformatic program permitted the design of a minor groove binder (MGB) TaqMan probe that specifically detected the LTMα15VH. We were able to demonstrate a broad range of rearranged VH gene copy number without any correlation to the state of health. Monitoring LTMα15VH gene copy number in a single donor over a period of 70 days revealed a time-related fluctuation of circulating B cells carrying LTMα15VH. We propose that our real-time PCR may serve as a model for the quantification of natural antibody sequences at a monoclonal level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 307, Issues 1–2, 20 December 2005, Pages 107-117
نویسندگان
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