کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902192 | 1545793 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of one VH antibody sequence in both healthy donors and urticaria patients
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کلمات کلیدی
CDRthreshold cycle numberMGBPBGDFcεRIαCIUNatural autoantibodyReal-time PCR - PCR به موقعBioinformatics - بیوانفورماتیک variable light chain - زنجیره سبک متغیرvariable heavy chain - زنجیره سنگین متغیرcomplementary determining region - منطقه تعریف مکملporphobilinogen deaminase - پورفبیلینوژن دامینازchronic idiopathic urticaria - کشاله ران ایدیوپاتیک مزمنMinor groove binder - گیربکس کوچک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
We have previously isolated anti-FcεRIα autoantibodies from phage libraries of healthy donors and urticaria patients. Strikingly, the same antibody, LTMα15, was isolated from both libraries. Sequence analysis revealed a germline configuration of the LTMα15 variable heavy (VH) chain with a slightly mutated variable light (VL) chain supporting its classification as a natural autoantibody. Distribution analysis of anti-FcεRIα autoantibodies by functional or serological tests delivered conflicting data. For this reason we have developed a new real-time PCR to analyse the distribution of LTMα15VH in healthy donors and urticaria patients. Our new bioinformatic program permitted the design of a minor groove binder (MGB) TaqMan probe that specifically detected the LTMα15VH. We were able to demonstrate a broad range of rearranged VH gene copy number without any correlation to the state of health. Monitoring LTMα15VH gene copy number in a single donor over a period of 70 days revealed a time-related fluctuation of circulating B cells carrying LTMα15VH. We propose that our real-time PCR may serve as a model for the quantification of natural antibody sequences at a monoclonal level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 307, Issues 1â2, 20 December 2005, Pages 107-117
Journal: Journal of Immunological Methods - Volume 307, Issues 1â2, 20 December 2005, Pages 107-117
نویسندگان
Michaela Fux, Monique Vogel, Michael B. Stadler, Beda M. Stadler, Sylvia M. Miescher,