کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902411 | 1545803 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA
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کلمات کلیدی
AAEMBLC1-INHAcute poststreptococcal glomerulonephritisAGNC1 inhibitor - C1 بازدارندهC3 Nephritic factor - C3 فاکتور خونیHereditary angioedema - آنژیوادم ارثیAcquired angioedema - آنژیوادما به دست آوردHAE - جستجوMannose-binding lectin - لکتین اتصال دهنده مانوزalternative pathway - مسیر جایگزینclassical pathway - مسیر کلاسیکMAC - مکmembrane attack complex - پیچیده حمله غشاء
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Primary defence against invading microorganisms depends on a functional innate immune system and the complement system plays a major role in such immunity. Deficiencies in one of the components of the complement system can cause severe and recurrent infections, systemic diseases, such as systemic lupus erythematosus (SLE) and renal disease. Screening for complement deficiencies in the classical or alternative complement pathways has mainly been performed by haemolytic assays. Here, we describe a simple ELISA-based format for the evaluation of three pathways of complement activation. The assays are based on specific coatings for each pathway in combination with specific buffer systems. We have standardized these assays and defined cut off values to detect complement deficiencies at the different levels of the complement system. The results demonstrate the value of these ELISA-based procedures for the functional assessment of complement deficiencies in clinical practice. The assay is now available commercially in kit form.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 296, Issues 1â2, January 2005, Pages 187-198
Journal: Journal of Immunological Methods - Volume 296, Issues 1â2, January 2005, Pages 187-198
نویسندگان
M.A. Seelen, A. Roos, J. Wieslander, T.E. Mollnes, A.G. Sjöholm, R. Wurzner, M. Loos, F. Tedesco, R.B. Sim, P. Garred, E. Alexopoulos, M.W. Turner, M.R. Daha,