Using oocytes for Wnt signaling assays: Paracrine assays and Wnt-conditioned medium
Keywords: شرایط محیطی; Xenopus; Oocyte; Wnt; Paracrine assay; Conditioned medium;
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Subtractive hybridization identifies genes differentially expressed by olfactory ensheathing cells and neural stem cells
Keywords: شرایط محیطی; Ensheathing cells; Neural precursor cell; Subtractive hybridization; Differential gene expression; Cell differentiation; Conditioned media; Conditioned medium;
Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury
Keywords: شرایط محیطی; AMI; acute myocardial Infarction; CM; conditioned medium; MI/R; myocardial ischemia/reperfusion; MSCs; mesenchymal stem cells; MWCO; molecular weight cut off; NCM; nonconditioned medium;
Research ArticleThe hepatitis C virus core protein indirectly induces alpha-smooth muscle actin expression in hepatic stellate cells via interleukin-8
Keywords: شرایط محیطی; HCV; hepatitis C virus; HSC; hepatic stellate cells; IL-8; interleukin-8; PCR; polymerase chain reaction; EF1; elongation factor 1; EGFP; empty and green fluorescent protein; SMA; smooth muscle actin; FCS; foetal calf serum; CM; conditioned medium; SD; st
Effects of an endothelial cell-conditioned medium on the hematopoietic and endothelial differentiation of embryonic stem cells
Keywords: شرایط محیطی; Conditioned medium; DiI-Ac-LDL; Embryonic stem cells; Endothelial cells; Hematopoietic cells;
Sparse plating increases the heterogeneity of proliferative potential of fibroblasts
Keywords: شرایط محیطی; Feeder layers; Low oxygen; Conditioned medium; Telomerase; Proliferative potential; Fibroblasts
Ovarian cancer cells stimulate uPA gene expression in fibroblastic stromal cells via multiple paracrine and autocrine mechanisms
Keywords: شرایط محیطی; CM; conditioned medium; UCM; unconditioned medium; rt-PCR; quantitative real time PCR; EGF; epidermal growth factor; HB-EGF; heparin-binding EGF-like growth factor; HGF; hepatocyte growth factor; IGF-I; insulin-like growth factor I; PDGF-BB; platelet deri
Proliferation of NS0 cells in protein-free medium: The role of cell-derived proteins, known growth factors and cellular receptors
Keywords: شرایط محیطی; NS0 myeloma cells; Autocrine growth factors; Regulation of proliferation; Conditioned medium; gp130
Neuroprotection from diazinon-induced toxicity in differentiating murine N2a neuroblastoma cells
Keywords: شرایط محیطی; Diazinon; Conditioned medium; GDNF; NFH; GAP-43; HSP-70
Effects of human marrow stromal cells on activation of microglial cells and production of inflammatory factors induced by lipopolysaccharide
Keywords: شرایط محیطی; BBB; blood-brain barrier; BDNF; brain-derived neurotrophic factor; CM; conditioned medium; CNS; central nervous system; ELISA; enzyme linked immunosorbent assay; HGF; hepatocyte growth factor; hMSCs; human MSCs; IGF-1; insulin-like growth factor-1; IFNγ;
A collagen-coated surface enables quantitative evaluation of morphological behaviors of rabbit chondrocytes relating to cell differentiation in an early culture phase
Keywords: شرایط محیطی; Collagen-coated surface; Chondrocyte behaviors; Conditioned medium; Aggregate formation; Cell differentiation; Image analysis
Novel Soluble Flt-1 Isoforms in Plasma and Cultured Placental Explants from Normotensive Pregnant and Preeclamptic Women
Keywords: شرایط محیطی; Villous explant culture; Conditioned medium; Preeclampsia; Plasma; Heparin-agarose; New soluble Flt-1 variants; sFlt-1; Deglycosylation; VEGF binding
Differentiation potential of human pancreatic stem cells for epithelial- and endothelial-like cell types
Keywords: شرایط محیطی; Pancreatic stem cells; Endothelial differentiation; Epithelial differentiation; In vitro angiogenesis; Direct coculture; Conditioned medium;
Cross talk Initiated by Endothelial Cells Enhances Migration and Inhibits Anoikis of Squamous Cell Carcinoma Cells through STAT3/Akt/ERK Signaling
Keywords: شرایط محیطی; CM; conditioned medium; CXCL8; interleukin-8; EBM; endothelial cell basal medium; EGF; epidermal growth factor; ERK; extracellular signal-regulated kinase; HDMEC; human dermal microvascular endothelial cell; HNSCC; head and neck squamous cell carcinoma; I
Direct contact between boar spermatozoa and porcine oviductal epithelial cell (OEC) cultures is needed for optimal sperm survival in vitro
Keywords: شرایط محیطی; Porcine; Oviductal epithelial cells (OECs); Co-culture; Conditioned medium; Sperm quality; Sperm capacitation
CD44v6 Dependence of Premetastatic Niche Preparation by Exosomes
Keywords: شرایط محیطی; ASMLwt; BSp73ASML; ASMLkd; CD44v4-v7 knockdown in ASML; BMC; bone marrow cell; C3; complement component 3; CD44v; CD44 variant isoform; CIC; cancer-initiating cell; Coll; collagen; CM; conditioned medium; FN; fibronectin; HA; hyaluronic acid; HAS3; HA syn
The mixed coculture effect of primary rat hepatocytes and bone marrow cells is caused by soluble factors derived from bone marrow cells
Keywords: شرایط محیطی; hepatocytes; bone marrow cells; coculture; conditioned medium; albumin; ammonia; spheroid
The anti-apoptotic activity associated with phosphatidylinositol transfer protein α activates the MAPK and Akt/PKB pathway
Keywords: شرایط محیطی; PI-TP; phosphatidylinositol transfer protein; PI; phosphatidylinositol; PC; phosphatidylcholine; PLA; phospholipase A; MAPK; mitogen-activated protein kinase; ERK; extracellular signalling related kinase; IAP; inhibitor of apoptosis protein; PKB; protein
Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling
Keywords: شرایط محیطی; conditioned medium; spinal cord injury; DAPT; BDNF; brain-derived neurotrophic factor; BrdU; 5-bromo2â²-deoxy-uridine; DMSO; dimethyl sulfoxide; FGF2; fibroblast growth factor-2; GDNF; glial cell-derived neurotrophic factor; GFAP; glial fibrillary acidic
Enhancement of adipogenesis induction by conditioned media obtained from cancer cells
Keywords: شرایط محیطی; Adipogenesis; Cancer; Cell-to-cell interactions; Conditioned medium; Intracellular lipid droplet
Methods of in vitro macrophage maturation confer variable inflammatory responses in association with altered expression of cell surface dectin-1
Keywords: شرایط محیطی; ARE; adenine-uridine-rich elements; BMDM; bone marrow-derived macrophages; CARD; caspase recruitment domain; CM; conditioned medium; DC; dendritic cells; ELISA; enzyme-linked immunosorbent assay; FGF-β; fibroblast growth factor-basic; FITC; fluorescein
Molecular pharmacology of adipocyte-secreted autotaxin
Keywords: شرایط محیطی; LPA; lyso-phosphatidic acid; LPC; lyso-phosphatidylcholine; ATX; autotaxin; Lyso-PLD; lyso-phospholipase D; CM; conditioned medium; CCM; concentrated conditioned media; tlc; thin layer chromatography; Autotaxin; Lyso-phosphatidylcholine; Inhibition; Purif
Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress
Keywords: شرایط محیطی; Adipose-derived stem cell; Conditioned medium; Antioxidant; Superoxide dismutase; Glutathione peroxidase; Human dermal fibroblast
Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo
Keywords: شرایط محیطی; Dendritic cells; Differentiation; Conditioned medium; Maturation;; T cell stimulation;; CD3/CD28;; Vaccine adjuvant
Murine Embryonic Stem Cell-Derived Pancreatic Acinar Cells Recapitulate Features of Early Pancreatic Differentiation
Keywords: شرایط محیطی; Ad; adenovirus; Amyl; α-amylase; CPA; carboxypeptidase A; CCK; cholecystokinin; ChymoB; chymotrypsinogen B; CM; conditioned medium; EB; embryoid bodies; Ela1; elastase 1; ES; embryonic stem; qRT-PCR; quantitative reverse-transcription polymerase chain re
Acceleration of mycelia growth by adding Grifola umbellata broth concentrate in solid-state and submerged cultures
Keywords: شرایط محیطی; Grifola umbellata; Conditioned medium; Stimulated effect; Solid-state culture; Submerged culture
Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia
Keywords: شرایط محیطی; conditioned medium of dorsal root ganglia; differentiation; embryonic stem cell; motor neuronBrdU; 5-bromo-2′-deoxyuridine; ChAT; choline acetyltransferase; CM; conditioned medium; DBH; dopamine β-hydroxylase; DRG; dorsal root ganglion; ES; embryonic stem
Stromal cells of endometriosis fail to produce paracrine factors that induce epithelial 17β-hydroxysteroid dehydrogenase type 2 gene and its transcriptional regulator Sp1: a mechanism for defective estradiol metabolism
Keywords: شرایط محیطی; 17β-hydroxysteroid dehydrogenase type 2; conditioned medium; endometriosis; endometrium; epithelium; estrone; paracrine; stroma; progesterone receptor; R5020; estradiol; specific protein-1;
TGF-β modulates β-Catenin stability and signaling in mesenchymal proliferations
Keywords: شرایط محیطی; TGF; transforming growth factor; APC; adenomatous polyposis coli; FCS; fetal calf serum; MEF; mouse embryonic fibroblast; BrdU; 5-bromo-2â²-deoxyuridine; IP; immunoprecipitation; TCF; T cell factor; α-SMA; alpha smooth muscle actin; CM; conditioned medi
Effect of conditioned medium of mouse embryonic fibroblasts produced from EC-SOD transgenic mice in nuclear maturation of canine oocytes in vitro
Keywords: شرایط محیطی; EC-SOD; Transgenic mouse; Mouse embryonic fibroblast; In vitro maturation; Canine oocyte; Conditioned medium;
The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells
Keywords: شرایط محیطی; Neuregulin; Epidermal growth factor; Transforming growth factor α; Conditioned medium; Lung development
Effects of the pig renal epithelial cell line LLC-PK1 and its conditioned medium on the phenotype of porcine microglia in vitro
Keywords: شرایط محیطی; Microglia; Cell culture; Conditioned medium; Proliferation; Phagocytosis; Superoxide production; Morphology
Frizzled-7 turnover at the plasma membrane is regulated by cell density and the Ca2+-dependent protease calpain-1
Keywords: شرایط محیطی; EC; endothelial cell(s); BAEC; bovine aortic endothelial cell(s); HUVEC; human umbilical vein endothelial cell; HEK293 cells; human embryonic kidney 293 cells; TCF; T-cell factor; Fz; Frizzled; LRP; low density lipoprotein-related receptor protein; ER; en
Osteocytes subjected to pulsating fluid flow regulate osteoblast proliferation and differentiation
Keywords: شرایط محیطی; Osteocyte; Osteoblast; Periosteal fibroblast; Proliferation; Differentiation; Mechanosensitivity; Conditioned medium; Bone cells; Fluid shear stress; Fluid flow
TNF induces the growth of thymocytes in rainbow trout
Keywords: شرایط محیطی; TNFα; Thymocyte; Macrophage; Rainbow trout; Cytokine; Recombinant TNFα; Conditioned medium; Proliferation;
Autocrine activation of DNA synthesis in prothoracic gland cells of the silkworm, Bombyx mori
Keywords: شرایط محیطی; Bombyx mori; Autocrine; Conditioned medium; Growth-promoting factor; DNA synthesis; Prothoracic gland cells; Biological function in vivo
Cav3.1 splice variant expression during neuronal differentiation of Y-79 retinoblastoma cells
Keywords: شرایط محیطی; alpha1G; splicing; T-type calcium current; Y-79 cells; differentiation; retinoblastoma; CM; conditioned medium; DMEM; Dulbecco's Modified Eagle Medium; GFAP; glial fibrillary acidic protein; HVA; high voltage-activated calcium channel; LVA; low voltage-
Neuregulin-1 isoform induces mitogenesis, KCa and Ca2+ currents in PC12 cells. A comparison with sciatic nerve conditioned medium
Keywords: شرایط محیطی; NRG-1, neuregulin-1; CM, conditioned medium; KCa, calcium-activated K+ currents; Kdr, K+ delayed rectifier current; CTX, charybdotoxin; IBX, iberiotoxin; KIA, A-currentsNeuregulin; Ionic channel; Conditioned medium; Large Ca-activated K channel; Survival;
Differentiation of mouse embryonic stem cells into neurons using conditioned medium of dorsal root ganglia
Keywords: شرایط محیطی; embryonic stem cell; differentiation; neuron; conditioned medium of dorsal root ganglia; nerve growth factor; ACM; astrocyte conditioned medium; BrdU; 5-bromo-2â²-deoxyuridine; CM; conditioned medium; DRG; dorsal root ganglion; ES; embryonic stem; LAU; l
Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons
Keywords: شرایط محیطی; Choroid plexus ependymal cells; Cerebrospinal fluid; In vitro; Growth/trophic factors; Conditioned medium;
Conditioned culture for protoplasts isolated from chrysanthemum: An efficient approach
Keywords: شرایط محیطی; Chrysanthemum; Protoplast preparation; Conditioned medium; Plating efficiency;
Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon)
Keywords: شرایط محیطی; ABA; abscisic acid; 6BAP; 6-benzylaminopurine; CM; conditioned medium; 2,4-D; 2,4-dichlorophenoxyacetic acid; EC; embryogenic cells; mCD medium; modified Campbell and Durzan's medium; PCV; packed cell volume after centrifugation at 100Â ÃÂ g; PSK; phytos
Human monocytes differentiate into macrophages under the influence of human KPB-M15 conditioned medium
Keywords: شرایط محیطی; BDCA; blood dendritic cell antibody; CFU; colony forming unit; CM; conditioned medium; DC; dendritic cells; EDTA; ethylenediaminetetraacetic acid; ELISA; enzyme-linked immunosorbent assay; FITC; fluorescein isothiocyanate; G-CSF; granulocyte colony-stimul
Sulf-2, a Proangiogenic Heparan Sulfate Endosulfatase, Is Upregulated in Breast Cancer
Keywords: شرایط محیطی; extracellular endosulfatase; Sulf-2; breast cancer; angiogenesis; heparan sulfate; CAM; chick chorioallantoic membrane; CM; conditioned medium; DCIS; ductal carcinoma in situ; ECM; extracellular matrix; FGF; fibroblast growth factor; FGFR; fibroblast grow
Immunomodulatory cytokines suppress epithelial nitric oxide production in inflammatory bowel disease by acting on mononuclear cells
Keywords: شرایط محیطی; NO; nitric oxide; IBD; inflammatory bowel disease; iNOS; inducible nitric oxide synthase; MMC; mixed mononuclear cells; CM; conditioned medium; IL-1ra; interleukin 1 receptor antagonist; Inflammatory bowel disease; Colonic epithelial cells; Nitric oxide;
Selective and efficient culturing of retinal pigment epithelial cells using a feeder layer
Keywords: شرایط محیطی; conditioned medium; feeder layer; retinal pigment epithelial cells; 3T3-J2;
Metalloproteinase activity is the sole factor responsible for the growth-promoting effect of conditioned medium in Trichoplusia ni insect cell cultures
Keywords: شرایط محیطی; Trichoplusia ni; Serum-free medium; Conditioned medium; Gel filtration fractions; Metalloproteinase activity; Autocrine regulation of proliferation;
Involvement of proinflammatory factors, apoptosis, caspase-3 activation and Ca2+ disturbance in microglia activation-mediated dopaminergic cell degeneration
Keywords: شرایط محیطی; PD; Parkinson's disease; DA; dopamine; CM; conditioned medium; LPS; lipopolysaccharide; VM; ventral mesencephalic; ara-C; β-d-arabinofuranoside; l-NIL; l-N6-1-iminoethyl-lysine hydrochloride; Microglia; Dopaminergic cell; Proinflammatory factors; Apoptos
In vitro formation and activity-dependent plasticity of synapses between Helix neurons involved in the neural control of feeding and withdrawal behaviors
Keywords: شرایط محیطی; mollusk; soma-soma cell culture; synaptogenesis; gap junctions; protein tyrosine kinases; short-term homosynaptic plasticity; ANOVA; analysis of variance; BSA; bovine serum albumin; CM; conditioned medium; DM; chemically defined medium; DMSO; dimethyl s