کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
22473 43291 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia
چکیده انگلیسی

Mouse embryonic stem (ES) cells have the pluripotent ability to differentiate in vitro into various cell lineages, including neurons. Adding chick dorsal root ganglion (DRG) conditioned medium (CM) to the culture medium promotes the differentiation of ES cells into neurons. We determined the types of neurons that differentiate from ES cells. The addition of DRG-CM caused nearly half of all ES cells on the periphery of the colony sphere to differentiate into neurons. Immunofluorescence analysis showed that the neurons that differentiated from ES cells were mainly motor, GABAergic, serotonergic, and cholinergic neurons. Of particular note, flow cytometry showed that approximately 50% of βIII-tubulin-positive neurons were motor neurons. This indicates that DRG-CM induces ES cells to differentiate into motor neurons as target of DRG neurons (sensory neurons).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 4, October 2007, Pages 257–262
نویسندگان
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