Keywords: ساختار چهارم; Disulfide engineering; Oligomeric proteins; Protein nanomaterials; Quaternary structure; GST; glutathione-S-transferase; H6-Lsmα; recombinant His-tagged variant of Lsmα protein from Methanobacterium thermoautotrophicum; IPTG; isopropyl-β-d-thiogalactop
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Keywords: ساختار چهارم; higher order structure; protein structure; secondary structure; tertiary structure; quaternary structure; biophysical methods; proteins;
Keywords: ساختار چهارم; higher order structure; protein structure; secondary structure; tertiary structure; quaternary structure; biophysical methods; proteins; structure stability; thermodynamics;
Keywords: ساختار چهارم; AMP; adenosine monophosphate; FBPase; fructose-1,6-bisphosphatase; FBP1; liver FBPase; FBP2; muscle FBPase; F1,6P2; fructose-1,6-bisphosphate; F6P; fructose-6-phosphate; FITC; fluorescein isothiocyanate; GSK3; glycogen synthase kinase 3; WT; wildtype; Fru
Keywords: ساختار چهارم; Human eukaryotic elongation factor 1 gamma; GST-like N-terminus domain; GSH-CDNB conjugation assay; Quaternary structure; ANS binding
Keywords: ساختار چهارم; Monooxygenase; Phenylacetate degradation pathway; Aromatic compounds degradation; Quaternary structure;
Keywords: ساختار چهارم; 3D-EM; three-dimensional electron microscopy; FU; functional unit; PDB; Protein Data Bank; EMDB; Electron Microscopy Data Bank; KLH; keyhole limpet hemocyanin; HdH; Haliotis diversicolor hemocyanin; MtH; Melanoides tuberculata hemocyanin; NpH; Nautilus po
Extraction of information on macromolecular interactions from fluorescence micro-spectroscopy measurements in the presence and absence of FRET
Keywords: ساختار چهارم; Fluorescence; Förster resonance energy transfer (FRET); Protein-protein interactions; Stoichiometry; Quaternary structure; Dissociation constant;
Modification of quaternary structure of Candida albicans GlcN-6-P synthase and its desensitization to inhibition by UDP-GlcNAc by site-directed mutagenesis
Keywords: ساختار چهارم; Candida albicans; Glucosamine-6-phosphate synthase; Quaternary structure; UDP-GlcNAc; Site-directed mutagenesis; DTT; dithiothreitol; Fru-6-P; d-fructose-6-phosphate; GAH; glutamine amidohydrolase domain; GlcN-6-P; d-glucosamine-6-phosphate; ISOM; ketose/
Muscarinic receptor oligomerization
Keywords: ساختار چهارم; Muscarinic acetylcholine receptor; Quaternary structure; Dimerization; Oligomerization; Ligand regulation; BRET; bioluminescence resonance energy transfer; CNO; clozapine-N-oxide; EL; extracellular loop; FCS; fluorescence correlation spectroscopy; FRET; f
Quaternary structure of human small heat shock protein HSPB6 (Hsp20) in crowded media modeled by trimethylamine N-oxide (TMAO): Effect of protein phosphorylation
Keywords: ساختار چهارم; Small heat shock proteins; Phosphorylation; Quaternary structure; Heterooligomeric complexes; Crowding agents; Self-association; AUC; analytical ultracentrifugation; DTT; dithiothreitol; GA; glutaraldehyde; ME; β-mercaptoethanol; PMSF; phenylmethane sulf
Involvement of the heterodimeric interface region of the nucleotide binding domain-2 (NBD2) in the CFTR quaternary structure and membrane stability
Keywords: ساختار چهارم; CFTR; NBD2; Dimerization; Membrane stability; Quaternary structure; Cystic fibrosis;
Insights on the structure of native CNF, an endogenous phospholipase A2 inhibitor from Crotalus durissus terrificus, the South American rattlesnake
Keywords: ساختار چهارم; Phospholipase A2 inhibitor; PLI; Phospholipase A2; PLA2; Crotalus; Quaternary structure;
Salt bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation
Keywords: ساختار چهارم; GR; Gloeobacter rhodopsin; BR; bacteriorhodopsin; HR; halorhodopsin; SRII; sensory rhodopsin II; PR; proteorhodopsin; XR; xanthorhodopsin; ESR; Exiguobacterium sibiricum rhodopsin; DDM; dodecyl-β-d-maltoside; SEC; size-exclusion chromatography; PSB; prot
Chimeric hepatitis E virus-like particle as a carrier for oral-delivery
Keywords: ساختار چهارم; Hepatitis E virus; Virus like particle; Recombinant capsid protein; HIV-1 epitope; Trypsin proteolysis; Quaternary structure;
The switch helix: A putative combinatorial relay for interprotomer communication in cGMP-dependent protein kinase
Keywords: ساختار چهارم; PKG; cGMP; Allostery; Crystal structure; cAMP; Quaternary structure
Effects of hydrostatic pressure on the quaternary structure and enzymatic activity of a large peptidase complex from Pyrococcus horikoshii
Keywords: ساختار چهارم; Aminopeptidase; Hydrostatic pressure; Large molecular complexes; Quaternary structure; Enzymatic activity; Hyperthermophiles
Allostery and the dynamic oligomerization of porphobilinogen synthase
Keywords: ساختار چهارم; Porphobilinogen synthase; Quaternary structure; Protein structure dynamics; Allostery; Morpheein
A tetrameric structure is not essential for activity in dihydrodipicolinate synthase (DHDPS) from Mycobacterium tuberculosis
Keywords: ساختار چهارم; DHDPS; Dihydrodipicolinate synthase; Quaternary structure; Tuberculosis;
Applications of analytical ultracentrifugation to protein size-and-shape distribution and structure-and-function analyses
Keywords: ساختار چهارم; Analytical ultracentrifugation; Band-forming ultracentrifugation; Active enzyme centrifugation; Sedimentation velocity; Size-and-shape distribution; Quaternary structure;
T-quaternary structure of oxy human adult hemoglobin in the presence of two allosteric effectors, L35 and IHP
Keywords: ساختار چهارم; Hemoglobin; Cooperativity; Allostery; NMR; Allosteric effectors; Quaternary structure
Characterization of monomeric dihydrodipicolinate synthase variant reveals the importance of substrate binding in optimizing oligomerization
Keywords: ساختار چهارم; (S)-ASA; (S)-aspartate-semialdehyde; DHDPR; dihydrodipicolinate reductase; DHDPS; dihydrodipicolinate synthase; HTPA; (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid; NAL; neuraminidate lyase; rmsd; root-mean-square deviation; Dihydrodipicolinate
Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3–RINGBox1 protein complex and elucidation of its quaternary structure with Keap1
Keywords: ساختار چهارم; Cullin3; Keap1; Nrf2; Rbx1; Codon-optimized; Quaternary structure; Stoichiometry
Exploring the dihydrodipicolinate synthase tetramer: How resilient is the dimer–dimer interface?
Keywords: ساختار چهارم; Dihydrodipicolinate synthase; Oligomer; Quaternary structure; Analytical ultracentrifugation; Protein interface; Protein–protein interaction
Modulation of quaternary structure of S100 proteins by calcium ions
Keywords: ساختار چهارم; AUC; equilibrium analytical ultracentrifugation; S100 proteins; Calcium binding; Quaternary structure; Sedimentation equilibrium; Structural changes;
Solution structures of 2 × 6-meric and 4 × 6-meric hemocyanins of crustaceans Carcinus aestuarii, Squilla mantis and Upogebia pusilla
Keywords: ساختار چهارم; Quaternary structure; Hemocyanin; Solution scattering; Oligomeric proteins
The leucine rich amelogenin protein (LRAP) adsorbs as monomers or dimers onto surfaces
Keywords: ساختار چهارم; Amelogenin; LRAP; Adsorption; Quaternary structure
Computational and mutagenesis studies of the streptavidin native dimer interface
Keywords: ساختار چهارم; Streptavidin; Quaternary structure; Molecular dynamics simulation; Solvent accessible surface area; Knob-into-hole interaction
Alpha crystallin: The quest for a homogeneous quaternary structure
Keywords: ساختار چهارم; alpha crystallin; small heat-shock proteins; quaternary structure; chaperone; cataract
The quaternary structure of Escherichia coli N-acetylneuraminate lyase is essential for functional expression
Keywords: ساختار چهارم; NAL, N-acetylneuraminate lyase; DHDPS, dihydrodipicolinate synthaseN-acetylneuraminate lyase; Quaternary structure; Enzyme kinetics; Protein stability; Functional expression
Kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe Fusobacterium nucleatum
Keywords: ساختار چهارم; Fusobacterium nucleatum; Glutamate racemase; Kinetics; Substrate specificity; Quaternary structure; Pseudosymmetry; pH activity; Mutagenesis; Bacillus subtilis
The dimeric assembly of Photobacterium leiognathi and Salmonella typhimurium SodC1 Cu,Zn superoxide dismutases is affected differently by active site demetallation and pH: An analytical ultracentrifuge study
Keywords: ساختار چهارم; Bacterial Cu,Zn superoxide dismutase; Quaternary structure; Metal occupancy; Long-range communication
Correlation between dissociation and catalysis of SARS-CoV main protease
Keywords: ساختار چهارم; SARS coronavirus; Protease; Mutation; Dimer interface; Quaternary structure; Analytical ultracentrifuge;
On the quaternary structure of a C-type lectin from Bothrops jararacussu venom – BJ-32 (BjcuL)
Keywords: ساختار چهارم; Oligomerization; Quaternary structure; Solution structure; Snake venom lectinBjcuL, Bothrops jararacussu lectin; CD, circular dichroism; EDTA, ethylenediaminetetraacetic acid; HPLC, high performance liquid chromatography; ESMS, electron spray mass spectro
Conserved Central Domains Control the Quaternary Structure of Type I and Type II Hsp40 Molecular Chaperones
Keywords: ساختار چهارم; Hsp, heat shock protein; SAXS, small-angle X-ray scattering; AUC, analytical ultracentrifugation; 3D, three-dimensionalHsp40; molecular chaperone; protein folding; quaternary structure; small-angle scattering
Protein dynamics explain the allosteric behaviors of hemoglobin
Keywords: ساختار چهارم; Hb, hemoglobin; MD, molecular dynamics; BPG, 2,3-biphosphoglycerate; MWC, Monod, Wyman and Changeux; KNF, Koshland, Nemethy and FilmerHemoglobin (Hb); Oxygen-binding; Allostery; Cooperativity; Quaternary structure; Molecular dynamics
Evolution of Quaternary Structure in a Homotetrameric Enzyme
Keywords: ساختار چهارم; DHDPS, dihydrodipicolinate synthase; DHDPR, dihydrodipicolinate reductase; SAXS, small-angle X-ray scatteringdihydrodipicolinate synthase; DHDPS; quaternary structure; enzyme dynamics
The molecular heterogeneity of hemocyanin: Structural and functional properties of the 4 × 6-meric protein of Upogebia pusilla (Crustacea)
Keywords: ساختار چهارم; EDTA; ethylene diamine tetra-acetic acid; EM; electron microscopy; FPLC; fast protein liquid chromatography; Hc; hemocyanin; SEC; size-exclusion chromatography; TEM; transmission electron microscopyHemocyanin; Quaternary structure; Dissociation; Oxygen bi
Despite its high similarity with monomeric arginine kinase, muscle creatine kinase is only enzymatically active as a dimer
Keywords: ساختار چهارم; Creatine kinase; Arginine kinase; Enzymatic activity; Quaternary structure; Folding; Unfolding;
A challenging insight on the structural unit 1 of molluscan Rapana venosa hemocyanin
Keywords: ساختار چهارم; Glycoprotein; Hemocyanin; Rapana venosa; Quaternary structure
The molecular heterogeneity of hemocyanin: Its role in the adaptive plasticity of Crustacea
Keywords: ساختار چهارم; Hemocyanin; Quaternary structure; Subunit; Crustacea; Oxygen binding; Molecular Heterogeneity; Phenotypic polymorphism
Stabilization of quaternary structure and activity of bovine liver catalase through encapsulation in liposomes
Keywords: ساختار چهارم; Bovine liver catalase; Liposomes; Thermal stability; Quaternary structure; Hydrogen peroxide decomposition
Comparative 11 Å structure of two molluscan hemocyanins from 3D cryo-electron microscopy
Keywords: ساختار چهارم; NpH, Nautilus pompilius hemocyanin; HtH1, Haliotis tuberculata hemocyanin isoform 1; OdH, Octopus dofleini hemocyanin; FU, functional unitHemocyanin; 3D-reconstruction; Quaternary structure; Electron microscopy; Mollusca; Cephalopoda; Gastropoda
Extracellular superoxide dismutase exists as an octamer
Keywords: ساختار چهارم; ECM, extracellular matrix; EC-SOD, extracellular superoxide dismutase; TUG-PAGE, Transverse urea-gradient PAGESuperoxide dismutase; Quaternary structure; Tissue distribution; Oxidative stress
Unique multimeric immunoglobulin crosslinking in four species from the family Gadidiae
Keywords: ساختار چهارم; Immunoglobulin; Quaternary structure; Gadid; Haddock; Cod
Nitro-thiocyanobenzoic acid (NTCB) reactivity of cysteines β100 and β110 in porcine luteinizing hormone: Metastability and hypothetical isomerization of the two disulfide bridges of its β-subunit seatbelt
Keywords: ساختار چهارم; pLH, porcine luteinizing hormone; NTCB, 2-nitro 5-thiocyanobenzoic acid; DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid; CID, collision-induced dissociationGlycoprotein hormone; Gonadotropin; Quaternary structure; Disulfide bridge; Isomerization
Expression, purification, and characterization of a new heterotetramer structure of leucyl-tRNA synthetase from Aquifex aeolicus in Escherichia coli
Keywords: ساختار چهارم; Leucyl-tRNA synthetase; Quaternary structure
High-level expression and purification of human thymidine kinase 1: Quaternary structure, stability, and kinetics
Keywords: ساختار چهارم; Human thymidine kinase 1; Expression; Purification; Kinetics; Quaternary structure; CD spectroscopy
Structural basis of the destabilization produced by an amino-terminal tag in the β-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus
Keywords: ساختار چهارم; Archaea; Glycoside hydrolase; Quaternary structure; Thermal stability; Infrared spectroscopy; Amide Iâ²; amide I band in a 2H2O medium; FT-IR; Fourier transform infrared; Ssβ-gly; recombinant Sulfolobus solfataricus β-glycosidase; Ssβ-gly-tag; recombi
Temperature dependence of chaperone-like activity and oligomeric state of αB-crystallin
Keywords: ساختار چهارم; αB-crystallin; SAXS; Chaperone activity; Heat shock protein; Protein aggregation; Quaternary structure