Identification, cloning and characterization of interleukin-17 and its family from zebrafish
Keywords: Zebrafish; Cytokines; IL-17; Cloning of cDNA; Expression; RT-PCR
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Variants of the 5â²-untranslated region of human NCF2: Expression and translational efficiency
Keywords: CGD; chronic granulomatous disease; DMEM; Dulbecco's Modified Eagle's Medium; FBS; fetal bovine serum; EST; expressed sequence tag; IFNγ; interferon γ; HUVEC; human vascular endothelial cells; NADPH; reduced nicotinamide-adenine dinucleotide phosphate;
Intraoperative localization of lymph node metastases with a replication-competent herpes simplex virus
Keywords: 10; 14; CT; computed tomography; GFP; green fluorescent protein; HSV; herpes simplex virus; LN; lymph node; MOI; multiplicity of infection; MPM; malignant pleural mesothelioma; PBS; phosphate-buffered saline; PET; positron emission tomography; RT-PCR; rev
Depletion of DNA methyltransferase 1 and/or DNA methyltransferase 3b mediates growth arrest and apoptosis in lung and esophageal cancer and malignant pleural mesothelioma cells
Keywords: 29; ASO; antisense oligo; DAC; 5 aza 2â² deoxycytidine; DNMT; DNA methyltransferase; HDAC; histone deacetylase; MM; mismatch; MPM; malignant pleural mesothelioma; NHBE cell; Normal human bronchial epithelial cell; RT-PCR; reverse transcription-polymera
Kinetic and molecular evidences that human cardiac muscle express non-M2 muscarinic receptor subtypes that are able to interact themselves
Keywords: Muscarinic receptor subtypes; Human heart; [3H]-QNB binding kinetics; Cooperative interactions; RT-PCR
Foot-and-mouth disease virus: A first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods
Keywords: Foot-and-mouth disease virus; Diagnosis; RT-PCR; Cell culture; Ring test; Virus detection
Increased expression of cysteinyl leukotriene receptor-1 in the brain mediates neuronal damage and astrogliosis after focal cerebral ischemia in rats
Keywords: cysteinyl leukotriene receptor; cerebral ischemia; neuronal damage; astrogliosis; pranlukast; cysteinyl leukotriene receptor antagonist; ANOVA; analysis of variance; CysLTs; cysteinyl leukotrienes; CysLT1 receptor; cysteinyl leukotriene receptor-1; ECA; e
Developmental pattern of M1 and M2 muscarinic gene expression and receptor levels in cat carotid body, petrosal and superior cervical ganglion
Keywords: peripheral chemoreceptors; muscarinic receptor; acetylcholine; development; cats; M1; muscarinic M1 receptor; M2; muscarinic M2 receptor; PBS; phosphate-buffered saline; PBS-T; phosphate-buffered saline with 0.1% Tween-20; RT-PCR; reverse transcriptase po
Transcriptomic profiling of the canine tachycardia-induced heart failure model: global comparison to human and murine heart failure
Keywords: Gene expression; Microarray; Cardiomyopathy; Transcriptome; Tachycardia-induced heart failure; RT-PCR; Gene ontology; Remodeling; Pacing
Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR
Keywords: Flow cytometry; Male germ cells; Microinsemination; RT-PCR; Spermatogenesis
Antibody generation through B cell panning on antigen followed by in situ culture and direct RT-PCR on cells harvested en masse from antigen-positive wells
Keywords: Monoclonal antibody; B cell; Plasma cell; B cell panning; B cell selection; B cell enrichment; Phage display; Hybridoma; Non-clonal; RT-PCR; SLAM
Effectiveness of 2009 pandemic influenza A(H1N1) vaccines: A systematic review and meta-analysis
Keywords: CI; confidence interval; LCI; laboratory-confirmed influenza illness; OR; odds ratio; RT-PCR; reverse-transcriptase polymerase chain reaction; Influenza; A(H1N1)pdm09; Vaccination; Effectiveness; Systematic review; Meta-analysis;
Nitric oxide modulates glutathione synthesis during endotoxemia
Keywords: LPS; lipopolysaccharide; OTZ; L-2-oxothiazolidine-4-carboxylic acid; GSH; glutathione; NOS; nitric oxide synthase; L-NAME; NÏ-nitro-L-arginine methyl ester; AMG; aminoguanidine; GSSX; acid-soluble oxidized glutathione; GCLh; γ-glutamylcysteine synthetas
Synaptic organization in cochlear inner hair cells deficient for the CaV1.3 (α1D) subunit of L-type Ca2+ channels
Keywords: calcium channel; hair cell; ribbon synapse; synaptogenesis; efferent synapse; BK channel; BK; large conductance calcium-activated potassium channel; CaV1.3; α subunit 1.3 forming L-type voltage-gated Ca2+ channels; cDNA; complementary DNA; CSP; cysteine
Through the central V2, not V1 receptors influencing the endogenous opiate peptide system, arginine vasopressin, not oxytocin in the hypothalamic paraventricular nucleus involves in the antinociception in the rat
Keywords: PVN; hypothalamic paraventricular nucleus; AVP; arginine vasopressin; OXT; oxytocin; L-Ek; leucine-enkephalin; M-Ek; methionine-enkephalin; β-Ep; β-endorphin; DynA1-13; dynorphinA1-13; ICC; immunocytochemistry; RT-PCR; reverse transcription-polyme
Epidermal Growth Factor Receptor Signaling Regulates Bax and Bcl-w Expression and Apoptotic Responses During Intestinal Adaptation in Mice
Keywords: bp; base pair; BrdU; 5-bromodeoxyuridine; EGF; epidermal growth factor; EGFR; epidermal growth factor receptor; EGFtg; transgenic epidermal growth factor-overexpressing mice; iFABP; intestinal fatty acid-binding protein; LCM; laser capture microdissec
CD8+ Cytotoxic T Cells Induce Relapsing Colitis in Normal Mice
Keywords: CD; Crohn's disease; CFSE; carboxy-fluorescein succinimidyl ester; CTL; cytotoxic T lymphocyte; DNBS; 2,4 dinitrobenzene sulfonic acid; DTH; delayed type hypersensitivity; ELISPOT; enzyme-linked immunospot; FACS; fluorescence-activated cell sorter; IBD;
Changes in response to spinal cord injury with development: Vascularization, hemorrhage and apoptosis
Keywords: blood vessel; cavitation; desmopressin; spinal cord regeneration; chick; secondary injury; DMSO; dimethyl-sulfoxide; E; embryonic day; H&E; hematoxylin and eosin; PBS; phosphate-buffered saline; RT-PCR; reverse transcription polymerase chain reaction; TUN
Developmental time course of potassium channel expression in the rat cochlear nucleus
Keywords: Auditory; Ion channel; RT-PCR; mRNA; QPCR;
Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: The balance between accuracy and precision
Keywords: Real-time PCR; RT-PCR; Error propagation; Amplification efficiency
Analysis of expression of secreted phospholipases A2 in mouse tissues at protein and mRNA levels
Keywords: AS; analytical sensitivity; BSA; bovine serum albumin; DTPA; diethylenetriaminepentaacetic acid; cps; counts per second; cv; coefficient of variation; G; group; IgG; Immunoglobulin G; LPS; lipopolysaccaride; PBS; phosphate buffered saline; PLA2; phospholi
Construction and characteristics of 3-end enriched cDNA library from individual embryos of cattle
Keywords: Cattle-embryo; cDNA library; RT-PCR; Gene expression
In vitro selection of halo-thermophilic RNA reveals two families of resistant RNA
Keywords: SELEX; Systematic Evolution of Ligands by Exponential enrichment; PCR; Polymerase Chain Reaction; dNTP; 2â²-deoxyribonucleotide 5â²-triphosphate; rNTP; ribonucleotide 5â²-triphosphate; Tris; tris(hydroxymethyl)aminomethane; EDTA; ethylene diamine tetra
A rodent model for allergic dermatitis induced by flea antigens
Keywords: RT-PCR; reverse transcriptase PCR; HPRT; hypoxanthine phosphoribosyl transferase; MTS; 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; PMS; phenazine methosulfate; PBST; phosphate buffer saline plus Tween 20; BSA;
Relevance of astrocytic activation to reductions of astrocytic GABAA receptors
Keywords: CNS; central nervous system; DIV; days in vitro; DMEM; Dulbecco's modified Eagle's medium; EGABA; reversal potential for GABA; ECl; reverse potential for Clâ; FCS; fetal calf serum; FITC; fluorescein isothiocyanate; GABA; γ-aminobutyric acid; GABAA; γ
Expression of discoidin domain receptor 1 during mouse brain development follows the progress of myelination
Keywords: carnosine; discoidin domain; myelin; oligodendrocytes; aa; amino acids; APC; anti-adenomatous polyposis coli; BCIP; 5-bromo-4-chloro-3-indolyl phosphate; CA II; carbonic anhydrase II; DDR1; discoidin domain receptor 1; DDR2; discoidin domain receptor 2; E
Analysis of thyroid hormone receptor βA mRNA expression in Xenopus laevis tadpoles as a means to detect agonism and antagonism of thyroid hormone action
Keywords: Xenopus laevis; Amphibian metamorphosis; Endocrine disruption; Thyroid hormone receptors; RT-PCR
Upregulation of the initiating step of the kynurenine pathway in postmortem anterior cingulate cortex from individuals with schizophrenia and bipolar disorder
Keywords: Tryptophan dioxygenase; Tryptophan pyrrolase; Gene expression; Metabolic pathway; Psychosis; HPLC; RT-PCR; Immunohistochemistry; Mental disorder
Practical experience of high throughput real time PCR in the routine diagnostic virology setting
Keywords: RT-PCR; Virus diagnosis; Sensitivity
Gene transcription profiling in pollutant exposed mussels (Mytilus spp.) using a new low-density oligonucleotide microarray
Keywords: Bp; base pair; Cat; catalase; Cyp-A; cyclophilin-A; Gst; glutathione transferase; Hsp; heat shock protein; Krs; stress responsive kinase; Mgd-1; Mytilus galloprovincialis defensin-1; M-MuLV Hâ RT; point mutated RNAse H (minus) Moloney Murine Leukemia Vi
Characterization of a highly virulent feline calicivirus and attenuation of this virus
Keywords: Positive-strand RNA virus; FCV; Calicivirus; Full-length capsid sequence; RT-PCR; Hypervariable region; Antigenic diversity; Serum-virus neutralization; Immunofluoresence; ELISA
Antiangiogenic effect of Lygodium flexuosum against N-nitrosodiethylamine-induced hepatotoxicity in rats
Keywords: Lygodium flexuosum; Antiangiogenic effect; N-Nitrosodiethylamine; Vascular endothelial growth factor; Angiopoietins 1 and 2; Tie-2; RT-PCR; Immunohistochemistry
Territrems B and C metabolism in human liver microsomes: Major role of CYP3A4 and CYP3A5
Keywords: Anti-CYP3A4 antibody; Chemical inhibitors; CYP3A4 cDNA-transfected V79 Chinese hamster cells; CYP3A5; O-Demethylation; 4β-C hydroxylation; Human liver microsomes; Immunoblotting; RT-PCR; Supersomes; Testosterone 6β-hydroxylation; Territrem B; Territrem
Molecular cloning and characterization of Schistosoma mansoni Ftz-F1 interacting protein-1 (SmFIP-1), a novel corepressor of the nuclear receptor SmFtz-F1
Keywords: bp; base pair; CBP; CREB binding protein; HAT; histone acetyltransferase; HDAC; histone deacetylase; GST; glutathione S-transferase; RACE; rapid amplification of cDNA ends; RT-PCR; reverse transcriptase-polymerase chain reaction; Schistosoma mansoni; SmFt
Pituitary Adenylyl Cyclase-Activating Polypeptide (PACAP) and its receptor (PAC1-R) in the cochlea: Evidence for specific transcript expression of PAC1-R splice variants in rat microdissected cochlear subfractions
Keywords: cochlea; RT-PCR; Western blot; immunohistochemistry; stria vascularis; tight junctionsAC, adenylyl cyclase; EP, endolymphatic potential; LW, lateral wall; OC, organ of Corti; PACAP, pituitary adenylyl cyclase-activating polypeptide; PACAP-27, the form of
Evaluation of various housekeeping genes for their applicability for normalization of mRNA expression in dioxin-treated rats
Keywords: 2,3,7,8-Tetrachlorodibenzo-p-dioxin; TCDD; Gene expression; Feed restriction; Housekeeping genes; Normalization methods; Strain differences; Microarray; RT-PCR; Real-time quantitative PCR
Chronic Myeloid Leukemia: Diagnosis and Treatment
Keywords: ALL; acute lymphoblastic leukemia; AML; acute myeloid leukemia; AP; accelerated phase; ATP; adenosine triphosphate; BP; blast phase; CCGR; complete cytogenetic response; CHR; complete hematologic response; CML; chronic myeloid leukemia; CP; chronic phase;
Long-term culture of muscle explants from Sparus aurata
Keywords: Fish; Muscle; Organ culture; Immunohistochemistry; RT-PCR; Growth factors
Differential and Epigenetic Gene Expression Profiling Identifies Frequent Disruption of the RELN Pathway in Pancreatic Cancers
Keywords: ApoER2; apolipoprotein E receptor 2; COBRA; combined bisulfite restriction analysis; 5Aza-dC; 5-aza-2â²-deoxycytidine; HDAC; histone deacetylase; IPMN; intraductal papillary mucinous neoplasm of the pancreas; MSP; methylation-specific polymerase chain re
Cellular, Molecular Consequences of Peroxisome Proliferator- Activated Receptor-δ Activation in Ovarian Cancer Cells
Keywords: Peroxisome proliferator-activated receptor δ; ovarian cancer; nuclear receptor; gene expression profiling; thiazolidinediones; EOC; epithelial ovarian cancer; FBS; fetal bovine serum; MTT; 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide; PP
Detection of classical swine fever virus in archival formalin-fixed tissues by reverse transcription-polymerase chain reaction
Keywords: Classical swine fever virus (CSFV); Diagnosis; RT-PCR; Formalin-fixed tissues;
Development of a reverse transcription-PCR assay to detect porcine circovirus type 2 transcription as a measure of replication
Keywords: Porcine circovirus; RT-PCR; Replication;
Estradiol regulates APP mRNA alternative splicing in the mice brain cortex
Keywords: APP; Alternative splicing; RT-PCR; Sex steroids; Aging; Mouse brain;
Detection of Chronic bee paralysis virus and Acute bee paralysis virus in Uruguayan honeybees
Keywords: Honeybees; Apis mellifera; Chronic bee paralysis virus; Acute bee paralysis virus; Honeybee mortality; RT-PCR;
Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
Keywords: chondrocyte; chondroitin sulfate; hyaluronic acid; RT-PCR;
Expression of IFITM1 in chronic myeloid leukemia patients
Keywords: Chronic myeloid leukemia; IFITM1; RT-PCR; Gene expression;
Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells
Keywords: Granulosa cells; mitochondria; RT-PCR;
Relationships between reverse transcriptase-polymerase chain reaction for prostate specific antigen, survival, and various prognostic laboratory factors in patients with hormone refractory prostate cancer
Keywords: RT-PCR; PSA-mRNA; Hormone refractory prostate cancer; Survival; Prognosis;
Protecting RNA in fixed tissue: An alternative method for LCM users
Keywords: Fixation protocol; LCM; Real-time PCR; RNA; RT-PCR;
Influence of maternal alcohol administration on c-Fos expression in the hippocampus of infant rats
Keywords: Maternal alcohol; c-Fos; Hippocampus; Immunohistochemistry; RT-PCR;