Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells
Keywords: Granulosa cells; mitochondria; RT-PCR;
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The expression of iASPP in acute leukemias
Keywords: AL; acute leukemia; ALL; acute lymphocytic leukemia; AML; acute myeloid leukemia; BM; bone marrow; CR; complete remission; EB; ethidium bromide; HAL; hybrid acute leukemia; iASPP; inhibitory member of the ASPP family; MNCs; mononuclear cells; PB; peripher
DARPP-32 and inhibitor-1 are expressed in pancreatic β-cells
Keywords: Insulin; Secretion; Exocytosis; Phosphatase; Dephosphorylation; Subcellular fractionation; PP1 inhibitor; Immunohistochemistry; RT-PCR;
Telmisartan inhibits expression of a receptor for advanced glycation end products (RAGE) in angiotensin-II-exposed endothelial cells and decreases serum levels of soluble RAGE in patients with essential hypertension
Keywords: AGEs; advanced glycation end products; RAGE; receptor for AGEs; ECs; endothelial cells; RAS; renin-angiotensin system; ACE; angiotensin-converting enzyme; Ang II; angiotensin II; ARBs; Ang II type 1 receptor blockers; ICAM-1; intracellular adhesion mole
Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages
Keywords: Lung; Digenea; Nitric oxide; Macrophage; Paragonimus mexicanus; Schistosoma bovis; iNOS; inducible nitric synthase; RT-PCR; reverse transcription-polymerase chain reaction; PBS; phosphate-buffered saline; L-NAME; nitro-l-arginine methyl ester;
Influence of maternal alcohol administration on c-Fos expression in the hippocampus of infant rats
Keywords: Maternal alcohol; c-Fos; Hippocampus; Immunohistochemistry; RT-PCR;
Protecting RNA in fixed tissue: An alternative method for LCM users
Keywords: Fixation protocol; LCM; Real-time PCR; RNA; RT-PCR;
Relationships between reverse transcriptase-polymerase chain reaction for prostate specific antigen, survival, and various prognostic laboratory factors in patients with hormone refractory prostate cancer
Keywords: RT-PCR; PSA-mRNA; Hormone refractory prostate cancer; Survival; Prognosis;
The nucleotide sequence of foot-and-mouth disease virus O/FRA/1/2001 and comparison with its British parental strain O/UKG/35/2001
Keywords: Foot-and-mouth disease virus; RT-PCR; Complete genome; FMDV O/FRA/1/2001;
Response of HIV positive patients to the long-term salvage therapy by lopinavir/ritonavir
Keywords: 3TC; lamivudin; ABC; abacavir; AZT; azidothymidin (zidovudin); d4Tstavudin; ddC; dideoxycytidin (zalcitabin); ddI; dideoxyinosin (didanosin); EFV; efavirenz; HAART; highly active antiretroviral therapy; IDV; indinavir; LPV; lopinavir; LPV/r; combination o
Detection of enterovirus RNA in cerebrospinal fluid (CSF) using NucliSens EasyQ Enterovirus assay
Keywords: CMV; cytomegalovirus; CSF; cerebrospinal fluid; ECL; electrochemiluminescence; f; female; HSV-1; herpes simplex virus type 1; HSV-2; herpes simplex virus type 2; EV; enterovirus; IC; internal homologous control; m; male; NASBA; nucleic acid sequence based
A comparison of Thermo Electron⢠RSV OIA® to viral culture and direct fluorescent assay testing for respiratory syncytial virus
Keywords: Viral culture; Direct fluorescent assay testing; RT-PCR;
The emergence of rotavirus genotype G9 in hospitalised children in Slovenia
Keywords: Rotavirus; RT-PCR; G-serotype;
Differentially expressed sequences from a cestode parasite reveals conserved developmental genes in platyhelminthes
Keywords: RDA; representational difference analysis; RT-PCR; reverse transcription polymerase chain reaction; Mesocestoides corti; Eucestoda; Tetrathyridia; Gene expression; Cestode strobilation;
Expression of the integrin genes in the developing cochlea of rats
Keywords: NF-κB; nuclear factor kappa B; EGF; epidermal growth factor; bFGF; basic fibroblast growth factor; ECM; extracellular matrix; PDGF; platelet-derived growth factor; RT-PCR; reverse transcription-polymerase chain reaction; DLU; digitalized light unit; RLU;
Molecular characterisation of a turbot Mx cDNA
Keywords: Mx; Scophthalmus maximus L; Turbot; Poly I:C; RT-PCR; Gene expression;
Aberrant expression of tumor suppressor genes and their association with chimeric oncogenes in pediatric acute lymphoblastic leukemia
Keywords: ALL; acute lymphoblastic leukemia; BM; bone marrow; PB; peripheral blood; TSG; tumor suppressor genes; RT-PCR; polymerase chain reaction with reverse transcription; Tumor suppressor genes; Chimeric oncogenes; Acute lymphoblastic leukemia; Pediatric; RT-PC
Association of the Protease Inhibitor Gene with Production Traits in Holstein Dairy Cattle
Keywords: candidate gene; quantitative trait loci; intragenic haplotype; dairy cow; DPR; daughter pregnancy rate; DYD; daughter yield deviation; PI; protease inhibitor; PL; productive life; QTG; quantitative trait gene; RT-PCR; reverse transcription-PCR; SNP; singl
Quantitative analysis of topoisomerase IIα to rapidly evaluate cell proliferation in brain tumors
Keywords: Quantitative analysis; Topoisomerase IIα; Brain tumor; RT-PCR;
Using genome-wide transcriptional profiling to elucidate small-molecule mechanism
Keywords: AML; acute myelogenous leukemia; RT-PCR; reverse transcription polymerase chain reaction; SFK; suppressor of FK506; TOR; target of rapamycin;
Diagnostic assays applied in BVDV control in The Netherlands
Keywords: BVDV; Control programme; ELISA; RT-PCR; Diagnostic assays;
Functional characterization of EhADH112: An Entamoeba histolytica Bro1 domain-containing protein
Keywords: Neor; neomycin resistance gene; PBS; phosphate-buffered saline solution; bp; base pairs; kb; kilobase pairs; PCR; polymerase chain reaction; RT-PCR; reverse transcriptase-polymerase chain reaction; EhADH112; EhCPADH; Alix/AIP1; Bro1 domain; Adhesin; Phago
Echinococcus multilocularis: Cloning and characterization of a member of the SNW/SKIP family of transcriptional coregulators
Keywords: Echinococcus multilocularis; Homo sapiens; Drosophila melanogaster; Caenorhabditis elegans; Schizosaccaromyces pombe; cestode; parasite; Ski interacting protein; Smad; small-mothers against decapentaplegic; TGF-β; transforming growth factor-β; ORF; open
Malate dehydrogenase activities are lower in some types of peripheral leucocytes of dogs and cats with type 1 diabetes mellitus
Keywords: Cat; Dog; Diabetes; Leucocytes; Malate dehydrogenase; RT-PCR;
Schistosoma japonicum: A method for transformation by electroporation
Keywords: Schistosome; Schistosoma japonicum; Transformation; Electroporation; Transgenic parasite; EGFP; enhanced green fluorescent protein; RT-PCR; reverse transcription-polymerase chain reaction; PBS; phosphate-buffered saline; MF-PBS; magnesium-free phosphate-b
Cloning and characterization of the hsp 18.55 gene, a new member of the small heat shock gene family isolated from wine Lactobacillus plantarum
Keywords: Wine; RT-PCR; Lactobacillus plantarum; Heat shock genes;
The potential of RNA interference as a tool in the management of viral hepatitis
Keywords: RNAi; RNA interference; siRNA; short-interfering ribonucleic acid; dsRNA; double-stranded ribonucleic acid; RISC; RNA-induced silencing complex; shRNA; short hairpin ribonucleic acid; ORF; open reading frame; RT-PCR; reverse transcription-polymerase chain
Changes in NMDA receptor expression in auditory cortex after learning
Keywords: Memory; Auditory perception; RT-PCR; RNA; Western blotting; Hearing;
Functional expression and adaptive regulation of Na+-dependent neutral amino acid transporter SNAT2/ATA2 in normal human astrocytes under amino acid starved condition
Keywords: Human astrocytes; System A; Neutral amino acid transporter SNAT2/ATA2; α-(Methylamino)isobutyric acid; Adaptive regulation; RT-PCR;
Biomarkers of adult and developmental neurotoxicity
Keywords: AMPH; amphetamine; METH; methamphetamine; DA; dopamine; 5-HT; serotonin; RT-PCR; reverse transcription-polymerase chain reaction; PLCo; posteriolateral cortical amygdaloid nucleus; NPY; neuropeptide Y precursor; IGF-1; insulin-like growth factor; IGFBP-1;
Molecular detection and serotypic characterization of dengue viruses by single-tube multiplex reverse transcriptase-polymerase chain reaction
Keywords: RT-PCR; Cell culture; Dengue virus;
Endotoxemia enhances expression of the signaling receptor (GP130) on protein and molecular level
Keywords: Endotoxin; IL-6; gp130; CD126; RT-PCR;
Gene expression and regulation of nerve growth factor in atopic dermatitis mast cells and the human mast cell line-1
Keywords: Neurotrophins; Atopic dermatitis; Cytokines; Immunohistochemistry; RT-PCR; Mast cells;
Quantitative Real-time Polymerase Chain Reaction Detection of Lymph Node Lung Cancer Micrometastasis Using Carcinoembryonic Antigen Marker
Keywords: lung cancer; micrometastasis; molecular staging; real-time reverse-transcriptase polymerase chain reaction; CEA; carcinoembryonic antigen; Ct; threshold cycle; mRNA; messenger RNA; NSCLC; non-small cell lung cancer; RT-PCR; reverse-transcription polymeras
Human metapneumovirus-associated respiratory tract infections in the Republic of Ireland during the influenza season of 2003-2004
Keywords: hMPV; human metapneumovirus; molecular epidemiology; phylogenetics; respiratory tract infections; RT-PCR;
Identification of Dobrava, Hantaan, Seoul, and Puumala viruses by one-step real-time RT-PCR
Keywords: Dobrava; Hantaan; Hantavirus; Puumala; Seoul; Real-time; TaqMan; RT-PCR;
A mRNA PCR for the diagnosis of feline infectious peritonitis
Keywords: Feline infectious peritonitis; Coronavirus; mRNA; RT-PCR;
Differential expression of the LGI and SLIT families of genes in human cancer cells
Keywords: GBM; glioblastoma multiforme; MMP; matrix metaloproteinasses; ADPEAF; autosomal dominant partial epilepsy with auditory features; Nb; Neuroblastoma; RCC; renal cell carcinoma; LGI1; leucine-rich, glioma inactivated-1; RT-PCR; reverse transcription polymer
Regulation of alternative splicing of caspase-2 through an intracellular signaling pathway in response to pro-apoptotic stimuli
Keywords: ASF/SF2; alternative splicing factor/splicing factor-2; ÎΨm; loss of mitochondrial membrane potential; GAPDH; reduced glyceraldehyde-phosphate dehydrogenase; hnRNPA1; heterogeneous nuclear ribonucleoprotein A1; JNK; c-Jun N-terminal kinase; MAPK; mitoge
Expression of Serotonin Receptors 2B and 4 in Human Prostate Cancer Tissue and Effects of Their Antagonists on Prostate Cancer Cell Lines
Keywords: Immunohistochemistry; RT-PCR; BrdU assay; Androgen-independent tumour cells;
Neuropeptide receptor transcripts are expressed in the rat clitoris and oscillate during the estrus cycle in the rat vagina
Keywords: ADNP; activity-dependent neuroprotective protein; ANG-2; angiotensin 2; AT1a; angiotensin receptor type 1a; CGRP; calcitonin gene-related peptide; CRLR; calcitonin receptor-like receptor; GAPDH; glyceraldehyde phosphate dehydrogenase; RAMP1; receptor acti
Functional divergence of duplicated c-myc genes in a tetraploid fish, the common carp (Cyprinus carpio)
Keywords: CAM1; carp c-myc1 encoding c-Myc1; CAM2; carp c-myc2 encoding c-Myc2; cDNA; DNA complementary to RNA; c-myc; gene(s) encoding c-Myc; c-Myc; cellular Myc; GST; glutathione S-transferase; Max; Myc associate protein X; RT-PCR; reverse transcriptase-PCR; Evol
Serogrouping of United States and some African serotypes of bluetongue virus using RT-PCR
Keywords: Bluetongue virus; dsRNA; RT-PCR; Molecular diagnostics;
Inflammatory cells as source of tachykinin-induced mucus secretion in chronic bronchitis
Keywords: Tachykinins; RT-PCR; In situ hybridisation; Immunohistochemistry; Human airways; Bronchitis;
Peritransplant Ischemic Injury Is Associated With Up-Regulation of Stromal Cell-Derived Factor-1
Keywords: FISH; fluorescent in situ hybridization; mRNA; messenger ribonucleic acid; RT-PCR; reverse transcription-polymerase chain reaction; SDF-1; stromal cell-derived factor-1;
The involvement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in atherosclerosis
Keywords: ACS; acute coronary syndrome; CRP; C-reactive protein; hsCRP; high-sensitivity C-reactive protein; LPC; lysophosphatidylcholine; oxLDL; oxidized low-density lipoprotein; mRNA; messenger ribonucleic acid; NCA; normal coronary arteries; PBMCs; peripheral bl
Gender Differences in Rat Aortic Smooth Muscle Cell Matrix Metalloproteinase-9
Keywords: AAA; abdominal aortic aneurysm; DMEM; Dulbecco's modified eagle medium; IL-1β; interleukin 1-beta; MMP-9; matrix metalloproteinase-9; NO; nitric oxide; OD; optical density; RASMCs; rat aortic smooth muscle cells; RT; reverse transcriptase; RT-PCR; real
Selection of control genes for quantitative RT-PCR based on microarray data
Keywords: Normalization; RT-PCR; Microarray; Housekeeping gene; Internal reference genes; Heart transplant;
The PcG protein HPC2 inhibits RBP-J-mediated transcription by interacting with LIM protein KyoT2
Keywords: RBP-J; recombination signal-binding protein-Jk; NIC; intracellular domain of Notch; EBNA2; Epstein-Barr virus nuclear antigen 2; PcG; polycomb group; HDAC; histone deacetylase; cDNA; complementary DNA; RT-PCR; reverse transcription polymerase chain reac
Molecular cloning and characterization of a cDNA encoding endonuclease from potato (Solanum tuberosum)
Keywords: cDNA; Endonuclease; Lotus japonicus; Potato; RT-PCR cloning; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; ORF; open reading frame; PCR; polymerase chain reaction; RT-PCR; reverse transcriptase polymerase chain reaction; SNP; single nucleotide polymorp