Detection of hepatitis A virus (HAV) in oysters (Crassostrea gigas)
Keywords: Oysters; HAV; RT-PCR; Hybridization
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Plasmodium chabaudi: Reverse transcription PCR for the detection and quantification of transmission stage malaria parasites
Keywords: Plasmodium chabaudi; Gametocyte; Malaria transmission; pcs230; Chronic infection; DNA; deoxyribonucleic acid; RNA; ribonucleic acid; dNTPs; deoxyribonucleotide triphosphate mix; RT-PCR; reverse transcription of the RNA followed by PCR amplification of the
Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain
Keywords: Amygdala; Hypothalamus; RT-PCR; Northern blot; Western blot; Immunohistochemistry;
Relative expression of D3 dopamine receptor and alternative splice variant D3nf mRNA in high and low responders to novelty
Keywords: Dopamine receptor; Drug dependence; RT-PCR; mRNA; Locomotor activity; Alternative splicing;
Investigation into the effect of soxR and soxS genes deletion on the central metabolism of Escherichia coli based on gene expressions and enzyme activities
Keywords: soxR; soxS; Batch culture; RT-PCR
Impact of interleukin-1 and interleukin-6 in murine primary schistosomiasis
Keywords: IL-1β; IL-6; Lung-stage schistosomula; Plasma lipids; RT-PCR; Schistosoma mansoni
Comparative non-radioactive RT-PCR assay: An approach to study the neurosteroids biosynthetic pathway in humans
Keywords: RT-PCR; PBR; 5α-Reductase 1; 3α-HSOR 1–2; Parkinson's disease; Neurosteroids; GC–MS
Differential expression of vascular endothelial growth factor, placenta growth factor and their receptors in placentae from pregnancies complicated by placenta accreta
Keywords: ELISA; Immunohistochemistry; Placenta growth factor; RT-PCR; Vascular endothelial growth factor; Vascular endothelial growth factor receptor; Western blot
RT-PCR analysis of the expression of POMC and its processing enzyme PC1 in amphibian melanotropes
Keywords: Frog; GAPDH; Melanotropes; NPY; POMC; PC1; RT-PCR; TRH
Cloning of a rat lung fibrogenic factor
Keywords: cDNA; complementary DNA; AMCase; acidic mammalian chitinase; bp; base pair; RT-PCR; transcriptase-polymerase chain reaction; RACE; rapid amplification of cDNA ends; iSBLP58; an inducible silicotic (rat) bronchoalveolar lavage protein-p58; iSPMF-p58; an in
Uneven evolutionary rates of bradykinin B1 and B2 receptors in vertebrate lineages
Keywords: aa, amino acid(s); bp, base pair(s); BK, bradykinin; B1, Bradykinin type 1 receptor; B2, Bradykinin type 2 receptor; cDNA, DNA complementary to RNA; kb, kilobase(s); Myr, million years; RT-PCR, Reverse transcriptase PCR; TM, transmembraneKininogen; RT-PCR
Galanin inhibits neural activity in the subfornical organ in rat slice preparation
Keywords: subfornical organ; galanin; immunoelectron microscopy; RT-PCR; extracellular recordingANGII, angiotensin II; DBB, diagonal band of Broca; DRG, dorsal root ganglion; GAL, galanin; LC, locus coeruleus; mEPSC, miniature excitatory postsynaptic current; mIPSC
An efficient and high-throughput approach for experimental validation of novel human gene predictions
Keywords: RT-PCR; Novel human genes
Induction of immunomodulator transcriptional responses by cholera toxin
Keywords: Cholera toxin; Gene expression; Transcription; Adjuvant; Inflammation; cDNA arrays; cAMP; IL-8; VEGF; RT-PCR; Real time PCR
Developmental Characteristics of Adapting Mouse Small Intestine Crypt Cells
Keywords: E; embryonic day; LCM; laser capture microdissection; P; postnatal day; RT-PCR; reverse-transcription polymerase chain reaction; SBR; small bowel resection; EST; expressed sequence tag; cDNA; complementary DNA;
Development and validation of an improved RT-PCR assay with nested universal primers for detection of hepatitis E virus strains with significant sequence divergence
Keywords: Hepatitis E virus; RT-PCR; HEV RNA; Genotype; Phylogenetic tree
A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance
Keywords: HIV-1; RT-PCR; Genotyping; Qualitative detection
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS)
Keywords: RT-LAMP; VHS; Diagnosis; SYBR Green I; RT-PCR
P2 receptor-stimulation influences axonal outgrowth in the developing hippocampus in vitro
Keywords: organotypic co-cultures; hippocampus; P2 receptor agonist; P2 receptor antagonist; ATP; fiber outgrowth; ACSF; artificial cerebrospinal fluid; DAB; diaminobenzidine hydrochloride; DIV; days in vitro; EC; entorhinal cortex; MAP2; microtubule-associated pro
Diurnal gene expression patterns of T-type calcium channels and their modulation by ethanol
Keywords: thalamus; epilepsy; spindle waves; sleep; dysrhythmia; BEC; blood ethanol concentration; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; NRSE; neuron-restrictive silencer element; RT-PCR; real time polymerase chain reaction; SCN; suprachiasmatic nucleus;
Molecular cloning and characterization of cecropin from the housefly (Musca domestica), and its expression in Escherichia coli
Keywords: Cecropin; Housefly (Musca domestica); Antimicrobial peptides; RT-PCR; in situ hybridization; Insect innate immunityaa, amino acid; DEPC, diethyl pyrocarbonate; GST, glutathion S-transferase; IPTG, isopropyl β-d-thiogalactoside; LB, Luria-Bertini; PBS, pho
A comprehensive survey of DNA-binding transcription factor gene expression in human fetal and adult organs
Keywords: Transcription factor; Expression survey; RT-PCR
Suppression of IL-6 production and proliferation by blocking STAT3 activation in malignant soft tissue tumor cells
Keywords: Tumor cells; Malignant fibrous histiocytoma; RT-PCR
Profiling 16S bacterial DNA and RNA: Difference between community structure and transcriptional activity in phenanthrene polluted sand in the vicinity of plant roots
Keywords: Bacterial community; DNA/RNA; Polycyclic aromatic hydrocarbon; Rhizodegradation; RT-PCR; TTGE;
Molecular analysis of a durum wheat ‘stay green’ mutant: Expression pattern of photosynthesis-related genes
Keywords: Durum wheat; RT-PCR; Senescence; ‘Stay green’cab, chlorophyll a/b binding protein gene; DAF, days after flowering; GDC, glycine decarboxylase; rbcS, rubisco small subunit gene; Rht, reduced height; RT-PCR, reverse transcription-polymerase chain reaction;
Detection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer with nested RT-PCR
Keywords: RT-PCR; Breast cancer; Circulating tumor cells; Peripheral blood; Mammaglobin A
Comparative analyses of genomic imprinting and CpG island-methylation in mouse Murr1 and human MURR1 loci revealed a putative imprinting control region in mice
Keywords: CGI; CpG island; DMR; differentially methylated region; COBRA; combined bisulfite restriction analysis; ICR; imprinting control region; RFLP; restriction-fragment length polymorphism; RT-PCR; reverse transcription-PCR; SNP; single nucleotide polymorphism;
Immune cell recruitment following acoustic trauma
Keywords: NIH; National Institutes of Health; ICAM-1; intercellular adhesion molecule - 1; RT-PCR; reverse transcription polymerase chain reaction; MCP-5 and MCP-1; macrophage chemotactic factor - 1 and - 5; MIP-1β; Macrophage inflammatory protein-1β; cd45;
Plasmodium yoelii: Axenic development of the parasite mosquito stages
Keywords: Plasmodium yoelii; Parasite mosquito stages; Malaria; Axenic; cs; circumsporozoite protein; cDNA; complementary deoxyribonucleic acid; DAPI,4â²,6-diamidino-2-phenylindole; DNA; deoxyribonucleic acid; ELISA; enzyme-linked immunosorbent assay; EMEM; Earleâ
The Magnitude of Early Castration-Induced Primary Tumour Regression in Prostate Cancer Does Not Predict Clinical Outcome
Keywords: Prostate cancer; PSA; Apoptosis; Proliferation; Micro-dissection; RT-PCR
Expression and function of pvcrt-o, a Plasmodium vivax ortholog of pfcrt, in Plasmodium falciparum and Dictyostelium discoideum
Keywords: CQ; chloroquine; CQS; chloroquine sensitivity; CQR; chloroquine resistance; DV; digestive vacuole; h; hour; hdhfr; human dihydrofolate reductase gene; IC50; 50% drug inhibitory concentration value; luc; luciferase gene; min; minutes; PbEF1α; intergenic r
Gene expression array analyses predict increased proto-oncogene expression in MMTV induced mammary tumors
Keywords: MMTV; Expression array; Proto-oncogene; RT-PCR
Molecular characterisation of cAMP-dependent protein kinase (PK-A) catalytic subunit isoforms in the male tick, Amblyomma hebraeum
Keywords: PK-A; protein kinase A (cAMP-dependent protein kinase); RT-PCR; reverse transcriptase-polymerase chain reaction; RACE; rapid amplification of cDNA ends; CAGE; crude accessory gland extract; Tick; Isoform; Transcript; Sperm; Protein kinase; Amblyomma hebra
Flock-prevalence of border disease virus infection in Basque dairy-sheep estimated by bulk-tank milk analysis
Keywords: Border disease virus; Antibody prevalence; RT-PCR; Dairy sheep; Bulk-tank milk; Spain
Effects of sarcolectin (SCL) on human peripheral blood mononuclear cells
Keywords: T cells; Cell proliferation; Costimulation; Cytokines; SCL; IFN; RT-PCR; NANA; N-acetyl neuraminic acid; ng; nanogram; P40; immunomodulatory compound extracted from Gram-positive Corynebacterium parvum; SCL; sarcolectin; VSV; vesicular stomatitis virus;
Response of the anaerobe Desulfovibrio vulgaris Hildenborough to oxidative conditions: proteome and transcript analysis
Keywords: Anaerobe; Sulfate-reducing bacteria; Oxidative stress; 2-DE gels; RT-PCR
Characterization of a partial α-amylase clone from red porgy (Pagrus pagrus): Expression during larval development
Keywords: Red porgy; Pagrus pagrus; α-amylase expression; In situ hybridization; RT-PCR; Development; Digestive physiology; Fish larvae; Pancreas
cDNA cloning and characterization of Type I procollagen α1 chain in the skate Raja kenojei
Keywords: cDNA cloning; Collagen; Elasmobranch; Embryo; Fish; Nucleotide sequence; RT-PCR; Skate
Molecular cloning and identification of mouse Cklfsf2a and Cklfsf2b, two homologues of human CKLFSF2
Keywords: CKLFSF; chemokine-like factor super family; CKLF; chemokine-like factor; CKLFSF1-8; chemokine-like factor super family member 1-8; GAPDH; glyceraldehydes-3-phosphate dehydrogenase; ORF; open-reading frame; MGD; mouse genome database; PCR; polymerase c
Molecular cloning and characterisation of peroxinectin, a cell adhesion molecule, from the giant freshwater prawn Macrobrachium rosenbergii
Keywords: Peroxinectin; Macrobrachium rosenbergii; Integrin binding motif; Lactococcus garvieae infection; RT-PCR
Comparative evaluation of RT-PCR, nucleic acid sequence-based amplification (NASBA) and real-time RT-PCR for detection of noroviruses in faecal material
Keywords: Norovirus; Detection; Faeces; Viral gastroenteritis; RT-PCR; NASBA; Real-time RT-PCR
Regulation of m1 muscarinic receptors and nNOS mRNA levels by autoantibodies from schizophrenic patients
Keywords: Autoantibodies; Cholinoceptors; Schizophrenia; Cerebral frontal cortex; ELISA procedure; RT-PCR; m1 mAChR-mRNA; nNOS-mRNA; Calcium/calmodulin; Phospholipase C
Regional specificity in deltamethrin induced cytochrome P450 expression in rat brain
Keywords: Deltamethrin; CYP; Brain regions; RT-PCR; Western blotting;
Cloning and expression of koala (Phascolarctos cinereus) liver cytochrome P450 CYP4A15
Keywords: CYP4A, gene encoding CYP4A; CYP, cytochrome P450; H19, male donor number 19; H21, male donor number 21; RACE, rapid amplification of cDNA ends; UPM, universal primer mixLauric acid hydroxylase; Eucalyptus diet; RT-PCR; Cos-7 cells; Sequence identity; Prot
Expression analysis of the sunflower SF21 gene family reveals multiple alternative and organ-specific splicing of transcripts
Keywords: NDRG, N-myc downstream regulated gene; DRG protein, developmentally regulated GTP-binding protein; HMW DNA, high molecular weight DNA; UTR, untranslated region.Helianthus annuus; RT-PCR; cDNA; Splice variants; Truncated proteins
Identification and characterization of RB-orthologous genes from the late blight resistant wild potato species Solanum verrucosum
Keywords: Late blight; Host resistance; Potato; Solanum verrucosum; Resistance gene cloning; PCR; polymerase chain reaction; RT-PCR; reverse transcription PCR;
A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses
Keywords: Polymerase chain reaction (PCR); Real-time PCR; RT-PCR; Internal control; Pestiviruses; Bovine viral diarrhea virus (BVDV)
Increased expression of cysteinyl leukotriene receptor-1 in the brain mediates neuronal damage and astrogliosis after focal cerebral ischemia in rats
Keywords: cysteinyl leukotriene receptor; cerebral ischemia; neuronal damage; astrogliosis; pranlukast; cysteinyl leukotriene receptor antagonist; ANOVA; analysis of variance; CysLTs; cysteinyl leukotrienes; CysLT1 receptor; cysteinyl leukotriene receptor-1; ECA; e
Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR
Keywords: Flow cytometry; Male germ cells; Microinsemination; RT-PCR; Spermatogenesis
Antibody generation through B cell panning on antigen followed by in situ culture and direct RT-PCR on cells harvested en masse from antigen-positive wells
Keywords: Monoclonal antibody; B cell; Plasma cell; B cell panning; B cell selection; B cell enrichment; Phage display; Hybridoma; Non-clonal; RT-PCR; SLAM