Keywords: scRNA; Therapeutic genome editing; Non-viral delivery; Nuclease-mediated genome editing; Oligonucleotide-mediated genome editing; CRISPR/Cas9; Peptide nucleic acid; BBB; blood brain barrier; CMV; cell-derived membrane vesicle; CRISPR; Clustered Regularly Intersp
مقالات ISI scRNA (ترجمه نشده)
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Keywords: scRNA; CRISPR/Cas9; GFP; Blasticidin S; Knockout and actin; EGFP; enhanced green fluorescent protein; Bsr; blasticidin S resistance; sgRNA; single-guide RNA; CRISPR/Cas9; Clustered Regularly Interspaced Short Palindromic Repeat-CRISPR-Associated protein 9;
Keywords: scRNA; Breeding methods; Chrysanthemum morifolium; CRISPR/Cas9; Genome editing; Ornamental plants; Transgenic technologies; 4CL; 4-coumarate:coenzyme A ligase; C3H; coumarate 3-hydroxylase; C4H; cinnamate 4-hydroxylase; CCD4; carotenoid cleavage dioxygenase 4; C
Keywords: scRNA; γTuRC; γ-tubulin ring complex; γTuSC; γ-tubulin small complex; CDA; citral dimethyl acetal; DMF; dimethyl fumarate; DAPI; 4â²,6â²-diamidino-2-phenyindole, dihydrochloride; sgRNA; single guide RNA; rest; sgRNA-resistant; shRNA; short hairpin RNA; shr
Keywords: scRNA; CRISPR-Cas9; Kluyveromyces lactis; Kluyveromyces marxianus; Scheffersomyces stipitis; Yarrowia lipolytica; Hansenula polymorpha; Pichia pastoris; HR; homologous recombination; NHEJ; nonhomologous end-joining; DSB; double strand break; CRISPR; Clustered re
Keywords: scRNA; Bombyx mori; Pigmentation; Guanylyl cyclase; Melanin; Integument; BC1; backcross 1; CREB; cAMP-response element binding protein; cGMP; cyclic guanosine monophosphate; GTP-CHI; GTP cyclohydrolase I; KO; knockout; MAP; mitogen-activated protein; PCR; polyme
Keywords: scRNA; CRISPR; Cas9; sgRNA; Guide RNA; Genome editing; Genome engineering
Keywords: scRNA; CRISPR/Cas9; U6 promoter; sgRNA; Transgene; Bombyx mori
The Implications of CRISPR-Cas9 Genome Editing for IR
Keywords: scRNA; CRISPR; Clustered Regularly Interspaced Short Palindromic Repeats; DSB; double-strand break; HDR; homology-directed repair; HR; homologous recombination; NHEJ; nonhomologous end-joining; PAM; protospacer-adjacent motif; sgRNA; single guide RNA;
Bombyx ortholog of the Drosophila eye color gene brown controls riboflavin transport in Malpighian tubules
Keywords: scRNA; ABC transporter; Riboflavin; CRISPR/Cas9; Gene knockout; Malpighian tubules; Silkworm; ABC; ATP-binding cassette; ABCG; ABC transporter G family; AP-3; Adaptor Protein-3; BLOC; biogenesis of lysosome-related organelles complex; BLOS2; biogenesis of lysoso
Application and development of genome editing technologies to the Solanaceae plants
Keywords: scRNA; Genome editing; Nuclease; Targeted mutagenesis; AGL; agamous-like; AID; activation-induced cytidine deaminase; ALOG; Arabidopsis LSH1 and Oryza G1; ANT; anthocyanin mutant; ARF; auxin-response factor; Aux; auxin-induced; BAK; brassinosteroid kinase; BOP;
In silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species
Keywords: scRNA; AbaI; Acinetobacter baumannii; CHOPCHOP; Crispr-cas9; sgRNA;
A splice junction-targeted CRISPR approach (spJCRISPR) reveals human FOXO3B to be a protein-coding gene
Keywords: scRNA; aa; amino acid(s); bp; base pairs; Cas9; CRISPR associated protein 9; cDNA; DNA complementary to RNA; CRISPR; Clustered Regularly Interspaced Short Palindromic Repeats; DMSO; dimethylsulfoxide; Dox; doxycycline; dsDNA; double stranded DNA; FOXO; forkhead
New applications of CRISPR/Cas9 system on mutant DNA detection
Keywords: scRNA; WT; wild type; MU; mutant type; CRISPR; Clustered Regularly Interspaced Short Palindromic Repeats; sgRNA; single-guide RNA; RGEN; Cas9-derived RNA-guided engineered nucleases; AFLPA; amplified fragment length polymorphism analysis; CRISPR/Cas9; Blocker; M
Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion
Keywords: scRNA; CRISPR/Cas9; clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9; FACS; fluorescence-activated cell sorting; sgRNA; single-guide RNA; T2A; Thoseaasigna virus 2A; EGFP; enhanced green fluorescent protein; ECFP; enhanced c
A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs
Keywords: scRNA; Cas9; Cas12a; PAM; sgRNA; crRNA; TXTL;
Diagnosis and therapy with CRISPR advanced CRISPR based tools for point of care diagnostics and early therapies
Keywords: scRNA; CRISPR; clustered regularly interspaced short palindromic repeats; Cas; CRISPR-associated protein; crRNA; CRISPR RNA; SgRNA; single guide RNA; tracrRNA; trans-activating RNA; PAM; protospacer-adjacent motif; DSB; DNA double strand break; SHERLOCK; Specifi
The interaction of IGF-1/IGF-1R and hydrogen sulfide on the proliferation of mouse primary vascular smooth muscle cells
Keywords: scRNA; BrdU; bromodeoxyuridine; CBS; cystathionine-beta-synthase; CRISPR; clustered regularly interspaced short palindromic repeats; CSE; cystathionine-gamma-lyase; DMEM; dulbecco's modified eagle's medium; ERK; extracellular regulated protein kinase; FBS; f
Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice
Keywords: scRNA; Acetolactate synthase (ALS); Activation-induced cytidine deaminase (AID); Herbicide tolerance; Rice; Selectable marker-free (SMF); Target-AID; Targeted nucleotide editing; CRISPR/Cas9; clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR as
The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells
Keywords: scRNA; CHO cells; Chinese Hamster Ovary cells; CRISPR; clustered regularly interspaced short palindromic repeat; sgRNA; single guide RNA; ST3GAL; alpha-2,3-sialyltransferase; ST6GAL; alpha-2,6-sialyltransferase; EPO; erythropoietin; Fc; Fragment crystallizable;
H2S protects lipopolysaccharide-induced inflammation by blocking NFκB transactivation in endothelial cells
Keywords: scRNA; H2S; Cystathionine gamma-lyase; LPS; Endothelial cells; Inflammation; NFκB; ChIP; chromatin immunoprecipitation; CRISPR; clustered regulatory interspersed short palindromic repeats; CSE; Cystathionine gamma-lyase; H2S; Hydrogen sulfide; HUVEC; Human umbi
The potential for targeted rewriting of epigenetic marks in COPD as a new therapeutic approach
Keywords: scRNA; COPD; Chronic obstructive pulmonary disease; CRISPR; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR/Cas9; Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated Protein 9 (Cas9); CSC; Cigarette smoke co
PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation
Keywords: scRNA; BLI; bioluminescence imaging; BSA; bovine serum albumin; CTL; cytotoxic T lymphocytes; CRISPR/Cas; Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated nucleases; DEGs; differently expressed genes; DMEM; Dulbecco's modified Eagle's
Intestinal Failure and Aberrant Lipid Metabolism in Patients With DGAT1 Deficiency
Keywords: scRNA; CDD; Genomic; PLE; 3-D Culture Model; B-LCL; B lymphoblastoid cell line; BSA; bovine serum albumin; CDD; congenital diarrheal disorder; cDNA; complementary DNA; DG; diacylglycerol; DGAT1; diacylglycerol-acyltransferase 1; hSI-EM; human small intestine exp
Genome-scale CRISPR pooled screens
Keywords: scRNA; Genome engineering; Genetic screens; CRISPR; Cas9; Noncoding; Cancer; RNAi; RNA interference; CRISPR; clustered regularly interspaced short palindromic repeats; sgRNA; single-guide RNA; DSB; double-strand break; NHEJ; non-homologous end-joining; PCR; poly
PAF53 is essential in mammalian cells: CRISPR/Cas9 fails to eliminate PAF53 expression
Keywords: scRNA; Ribosomal RNA; Nucleolus; RNA polymerase I; rDNA; PAF53; A49; CRISPR/Cas9; Transcription; rDNA; ribosomal DNA; sgRNA; small guide RNA; CRISPR; clustered regularly interspaced short palindromic repeats; Cas9; CRISPR-associated protein-9 nuclease; Pol I; RN
Advancing the design and delivery of CRISPR antimicrobials
Keywords: scRNA; Bacteriophage; Cas9; CRISPR-Cas systems; sgRNA; Tail-fiber proteins;
Systematic Analysis of Known and Candidate Lysine Demethylases in the Regulation of Myoblast Differentiation
Keywords: scRNA; H3K4me1; H3 lysine 4 monomethylation; H3K27ac; H3K27 acetylation; KMT; lysine methyltransferase; KDM; lysine demethylase; CRISPR; cluster regulatory interspaced short palindromic repeats; sgRNA; small guide RNA; myHC; myosin heavy chain; ALP; alkaline pho
ResourceEvaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona
Keywords: scRNA; sgRNA; Cardiopharyngeal mesoderm; Tunicate;
An in vitro Model to Mimic Selection of Replication-Competent HIV-1 Intersubtype Recombination in Dual or Superinfected Patients
Keywords: scRNA; RT; reverse transcriptase; CRF; circulating recombinant form; URF; unique recombinant form; C1; conserved domain 1; NGS; next generation sequencing; FRP; functional recombinant production; sgRNA; subgenomic RNA; nfl; near full-length; VSV-G; vesicular sto
SNORA21 - An Oncogenic Small Nucleolar RNA, with a Prognostic Biomarker Potential in Human Colorectal Cancer
Keywords: scRNA; CRC; colorectal cancer; ncRNAs; non-coding RNAs; miRNAs; microRNAs; lncRNAs; long non-coding RNAs; rRNAs; ribosomal RNAs; snoRNAs; small nucleolar RNAs; snoRNPs; small nucleolar ribosonucleoproteins; FFPE; formalin-fixed paraffin-embedded; NM; normal muco
Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice
Keywords: scRNA; Liver Cancer; Mouse Model; CRISPR Screen; Tumor Suppressor Genes; GAP; GTPase-activating protein; HCC; hepatocellular carcinoma; MAPK; mitogen-activated protein kinase; mRNA; messenger RNA; PCR; polymerase chain reaction; sgRNA; single-guide RNA; shRNA; s
Functional characterization of Pol III U6 promoters for gene knockdown and knockout in Plutella xylostella
Keywords: scRNA; CRISPR/Cas9; Diamondback moth; Lepidoptera; Pest control; shRNA; sgRNA;
Exploring the potential of genome editing CRISPR-Cas9 technology
Keywords: scRNA; Cas; CRISPR-associated proteins; cccDNA; covalently closed circular DNA; CCR5; C-C chemokine receptor type 5; CHO; Chinese hamster ovary; CRISPRs; clustered regularly interspaced short palindromic repeats; CRISPRa; CRISPR activation; CRISPRi; CRISPR inter
CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B
Keywords: scRNA; CRIPSR/Cas9; Pladienolide B; Spliceosome; Pre-mRNA splicing; Drug target validation; Drug discovery; SSNs; Site Specific Nucleases; ZFN; Zing Finger Nucleases; TALENs; Transcription Like Effector Nucleases; CRISPR; Clustered Regulatory Interspaced Short P
Controlling transcription in human pluripotent stem cells using CRISPR-effectors
Keywords: scRNA; CAG; hybrid of cytomegalovirus immediate early enhancer, chicken β-actin promoter and intron, rabbit β-globin splice acceptor; Cas; CRISPR-associated; CRISPR; clustered regularly interspaced short palindromic repeats; crRNA; CRISPR-derived RNA; dCas9; n
Hepatitis E Virus Mutations: Functional and Clinical Relevance
Keywords: scRNA; HEV; hepatitis E virus; ORF; open reading frame; MeT; methyltransferase; Y; Y-domain; PCP; papain-like cysteine protease; HVR; hypervariable region; X-domain; macro-domain; Hel; RNA helicase; RdRp; RNA-dependent RNA polymerase; PPR; polyproline region; CP
Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9
Keywords: scRNA; Genome editing; CRISPR; Cas9; Mutation; Indel; sgRNA; Double stranded breaks
CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production
Keywords: scRNA; CRISPR; Clustered regularly interspaced short palindromic repeats; CRISPRi; Clustered regularly interspaced short palindromic repeats interference; MVA; mevalonate; sgRNA; single guide RNA; dCas9; inactive Cas9; IPP; isopentenyl diphosphate; DMAPP; dimeth
lncRNA in the liver: Prospects for fundamental research and therapy by RNA interference
Keywords: scRNA; Long noncoding RNA (lncRNA); Delivery; siRNA; Liver; GalNAc conjugate; Lipid nanoparticle (LNP); Endosome escape; RNAi; siRNA; Hepatocellular carcinoma; ASGPR; asialoglycoprotein receptor; CRISPR-Cas; clustered regularly interspaced short palindromic re
Advances in therapeutic CRISPR/Cas9 genome editing
Keywords: scRNA; Cas9; CRISPR-associated protein 9; cccDNA; covalently closed circular DNA; CCR5; chemokine receptor 5; CFTR; cystic fibrosis transmembrane conductance regulator; CRISPRs; clustered regularly interspaced short palindromic repeats; crRNA; CRISPR RNA; Crygc;
CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention
Keywords: scRNA; OA; osteoarthritis; ECM; extracellular matrix; HA; hyaluronan; HAS2; hyaluronan synthase-2; RCS; rat chondrosarcoma; sgRNA; single guide RNA; sGAG; sulfated glycosaminoglycan; DMMB; dimethylmethylene blue; Hyaluronan; Hyaluronan synthase-2; Aggrecan; Chon
Current status of potential applications of repurposed Cas9 for structural and functional genomics of plants
Keywords: scRNA; CRISPR; Crop improvement; dCas9; Epigenetics; Gene editing; Gene regulation; CRISPRa; CRISPR activation; CRISPR-Cas; Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR associated proteins; CRISPRi; CRISPR interference; crRNA; CRISPR RNA; D
Advancements in zebrafish applications for 21st century toxicology
Keywords: scRNA; CRISPR; Clustered, regularly interspaced, short palindromic repeats; Dpf; Days post fertilization; DSB; Double strand break; Hpf; Hours post fertilization; HSC; Hematopoietic stem cell; HTS; High-throughput screen; KO; Knockout; MO; Morpholino oligonucleo
A Genome-Wide CRISPR Library for High-Throughput Genetic Screening in Drosophila Cells
Keywords: scRNA; CRISPR/Cas9; Genome-wide library; Drosophila; Cas9; CRISPR associated protein 9; CRISPR; clustered regularly interspaced short palindromic repeat; HTS; high throughput sequencing; sgRNA; single guide RNA;
A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila
Keywords: scRNA; CRISPR; Genome editing; Drosophila; Cas9; sgRNA; Off-target
Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9
Keywords: scRNA; 3-BrPA; 3 bromopyruvate; AOP; adverse outcome pathway; ARF4; ADP-ribosylation factor 4; BFA; brefeldin A; BT; 1,2,4-benzenetriol; CML; chronic myeloid leukemia; COSMID; CRISPR off-target sites with mismatches, insertions, and deletions; CRISPR-Cas9; clust
Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity
Keywords: scRNA; CRISPR; clustered regularly interspaced palindromic repeats; Cas9; CRISPR associated protein 9; crRNA; CRISPR RNA; tracrRNA; trans-activating CRISPR RNA; PAM; protospacer adjacent motif; sgRNA; single guide RNA; NGS; next generation sequencing; PSC; pluri
Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies
Keywords: scRNA; Leukemia; Multiple myeloma; RNA therapy; TRUE gene silencing; tRNase ZL; sgRNA;
Expanding the utility of heptamer-type sgRNA for TRUE gene silencing
Keywords: scRNA; Heptamer; Locked nucleic acid; sgRNA; tRNase ZL; TRUE gene silencing;