کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8956069 | 1646127 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice
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کلمات کلیدی
SMFActivation-induced cytidine deaminase (AID)ImazamoxAcetolactate synthase (ALS)single-guide RNAActivation-induced cytidine deaminasehptNHEJPAMCRISPR/Cas9 - CRISPR / Cas9sgRNA - scRNAacetolactate synthase - استاتولاکتانت سنتازRice - برنجALS - بیماری اسکلروز جانبی آمیوتروفیکherbicide tolerance - تحمل علف کشnon-homologous end joining - عدم پیوستن انتهای غیر همولوگIMZ - قلمرو کوهستانیprotospacer adjacent motif - موتیف مجاور protospacerHomologous recombination - نوترکیبی همولوگhygromycin phosphotransferase - هیگزیدرومایسین فسفاتانسفرازAID - کمک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or DNA modification enzymes. Target-AID is a synthetic complex of nuclease deficient Cas9 fused to an activation-induced cytidine deaminase (AID) that enables targeted nucleotide substitution (C to T or G to A). We previously demonstrated that the introduction of desired point mutations into target genes by Target-AID confers herbicide tolerance to rice callus. Inheritance of the introduced mutations, as well as the removal of transgenes, are key issues that must be addressed in order to fully develop Target-AID as a plant breeding technique. Here we report the transmission of such mutations from the callus to regenerants and their progenies, leading to a generation of selectable marker-free (SMF) herbicide tolerant rice plants with simultaneous multiplex nucleotide substitutions. These findings demonstrate that Target-AID can be developed into novel plant breeding technology which enables improvement of multiplex traits at one time in combination with sophisticated targeted base editing with the simplicity and versatility of CRISPR/Cas9 system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 131, October 2018, Pages 78-83
Journal: Plant Physiology and Biochemistry - Volume 131, October 2018, Pages 78-83
نویسندگان
Zenpei Shimatani, Ushio Fujikura, Hisaki Ishii, Yusuke Matsui, Minoru Suzuki, Yuki Ueke, Ken-ichiro Taoka, Rie Terada, Keiji Nishida, Akihiko Kondo,