Keywords: سایت موتاگنسیس را هدایت می کند; Triosephosphate isomerase deficiency; Site directed mutagenesis; Recombinant enzyme; Activity; Stability; Crystal structure;
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Keywords: سایت موتاگنسیس را هدایت می کند; hFGF1; human fibroblast growth factor-1; SDM; site directed mutagenesis; CD; circular dichroism; HSQC; heteronuclear single quantum coherence; ATCC; American Type Culture Collection; VMD; Visual Molecular Dynamics; ITC; Isothermal titration calorimetry; F
Keywords: سایت موتاگنسیس را هدایت می کند; Aequorin; Site directed mutagenesis; Luminescence; Double mutant;
Keywords: سایت موتاگنسیس را هدایت می کند; Biocatalysts; Directed evolution; Immobilisation; Nanoparticles; Site directed mutagenesis;
Keywords: سایت موتاگنسیس را هدایت می کند; Filarial parasite; Macrophage Migration Inhibitory Factor; CXXC motif; Oxidoreductase activity; Homology modeling; Site directed mutagenesis
Keywords: سایت موتاگنسیس را هدایت می کند; Peroxidase activity; Resonance Raman; Circular dichroism; Ligand binding kinetics; Site directed mutagenesis; ApoptosisApaf 1, apoptotic protease-activating factor 1; Cyt c, cytochrome c; CL, cardiolipin; CD, circular dichroism; CT, charge transfer; CT1,
Keywords: سایت موتاگنسیس را هدایت می کند; AOX1; aldehyde oxidase 1; mAOX3; mouse aldehyde oxidase 3; Methotrexate (PubChem CID: 5484402); 7-Hydoxymethotrexate (PubChem CID: 126941); Aldehyde oxidase; Methotrexate; Drug metabolism; Enzyme kinetics; Homology modelling; Site directed mutagenesis;
Keywords: سایت موتاگنسیس را هدایت می کند; 2â²-O-meNTP; 2â²-O-methylribonucleoside triphosphates; aa; amino acid; CBM; cellulose binding module; CBMâ¢; combinatorial beneficial mutagenesis; CGTase; cyclodextrin glycosyltransferase; CODH; carbon monoxide dehydrogenase; CSAT; combinatorial active
Keywords: سایت موتاگنسیس را هدایت می کند; CES, carboxylesterases; hCES, human carboxylesterasesCarboxylesterase; Deglycosylation; Glycosylation; hCES; Site directed mutagenesis
Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis: Binding studies, site-directed mutagenesis and stereoselectivity
Keywords: سایت موتاگنسیس را هدایت می کند; Cylindrospermopsin; 2-Oxoglutarate iron-dependent hydroxylase; Fluorescence; Iron binding; Site directed mutagenesis; 2OG; 2-oxoglutarate; 7-deoxy-CYN; 7-deoxy-cylindrospermopsin; 7-epi-CYN; 7-epi-cylindrospermopsin; CYN; cylindrospermopsin; DTT; dithioth
Improving the thermostability of Serratia marcescens B4A chitinase via G191V site-directed mutagenesis
Keywords: سایت موتاگنسیس را هدایت می کند; DNS; 3, 5-dinitrosalicylic acid; PDB; Protein Data Bank; WT; wild type; QCSDM; quick-change site-directed mutagenesis; IPTG; isopropyl-c-D-thiogalactoside; Km; Michaelis constant; Vmax; maximum velocity; RMSD; root-mean-square deviation; β chain; Chitina
Probing the role of proline â135 on the structure, stability, and cell proliferation activity of human acidic fibroblast growth factor
Keywords: سایت موتاگنسیس را هدایت می کند; Fibroblast growth factor; Heparin binding; Charge-reversal; Bioactivity; Stability; human acidic fibroblast growth factor-1; hFGF1; wildtype hFGF1; wt-hFGF1; site directed mutagenesis; SDM; circular dichroism; CD; heteronuclear single quantum coherence; H
Mutagenesis of threonine to serine in the active site of Mycobacterium tuberculosis fructose-1,6-bisphosphatase (Class II) retains partial enzyme activity
Keywords: سایت موتاگنسیس را هدایت می کند; glpX; Mycobacterium tuberculosis; Fructose-1,6-bisphosphatase; Lithium sensitive enzyme; Gluconeogenesis; Site directed mutagenesis;
Rigidifying flexible sites: An approach to improve stability of chondroitinase ABC I
Keywords: سایت موتاگنسیس را هدایت می کند; Chondroitinase ABC I; Site directed mutagenesis; Stability; Flexibility and B-factor;
The Met80Ala and Tyr67His/Met80Ala mutants of human cytochrome c shed light on the reciprocal role of Met80 and Tyr67 in regulating ligand access into the heme pocket
Keywords: سایت موتاگنسیس را هدایت می کند; Cyt c; cytochrome c; CL; cardiolipin; CD; circular dichroism; CT; charge transfer; CT1; charge transfer transition [a2u(Ï)âeg(dÏ)]; WT; wild type; MA; Met80Ala human cytochrome c mutant; YH; Tyr67His human cytochrome c mutant; YR; Tyr67Arg human cytoc
Enhanced catalysis of l-asparaginase from Bacillus licheniformis by a rational redesign
Keywords: سایت موتاگنسیس را هدایت می کند; l-asparaginase; B. licheniformis; Site directed mutagenesis; Enhanced catalysis
Arginine kinase in Toxocara canis: Exon-intron organization, functional analysis of site-directed mutants and evaluation of putative enzyme inhibitors
Keywords: سایت موتاگنسیس را هدایت می کند; Toxocara canis; Arginine kinase; Gene structure; Site directed mutagenesis; Inhibition kinetics;
A chimeric human APOBEC3A protein with a three amino acid insertion confers differential HIV-1 and adeno-associated virus restriction
Keywords: سایت موتاگنسیس را هدایت می کند; APOBEC3A; HIV-1; Parvovirus; AAV-2; Virus restriction; Site directed mutagenesis; AC-Loop 1; Protein structure;
Comparative analysis of point mutations on protein COOH terminal near surface and its hydrophobic core provide insights on thermostability of Bacillus Lipase LipJ
Keywords: سایت موتاگنسیس را هدایت می کند; WT; wild type; IPTG; isoprpyl-β-thiogalactopyranoside; LB; Luria Bertani; pNP; para-nitrophenol; PIC; protein interaction calculator; BLAST; basic local alignment search tool; Bacillus lipase; Thermostability; Site directed mutagenesis; Homolog modeling;
Arg-265: A critical residue of L.donovani cytosolic SHMT in maintaining the binding of THF and catalysis
Keywords: سایت موتاگنسیس را هدایت می کند; Cytosolic serinehydroxymethyltransferase; Pyridoxal-5-phosphate; Site directed mutagenesis; Tetra hydrofolate; Leishmania donovani;
An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants
Keywords: سایت موتاگنسیس را هدایت می کند; Dye decolourising peroxidase; Pseudomonas putida; Site directed mutagenesis; Kinetics; Spectroscopy; Redox properties;
The a subunit of the A1AO ATP synthase of Methanosarcina mazei Gö1 contains two conserved arginine residues that are crucial for ATP synthesis
Keywords: سایت موتاگنسیس را هدایت می کند; DES; diethylstilbestrol; DCCD; N,Nâ²-dicyclohexylcarbodiimide; DDM; n-dodecyl-β-d-maltoside; DTE; dithioerythritol; IPTG; Isopropyl-β-d-1-thiogalactopyranoside; IMVs; inverted membrane vesicles; LILBID-MS; laser induced liquid beam ion desorption mass
A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors
Keywords: سایت موتاگنسیس را هدایت می کند; WCI; winged bean chymotrypsin inhibitor; ETI; Erythrina caffra trypsin inhibitor; STI; soybean trypsin inhibitor; BPTI; bovine pancreatic trypsin inhibitor; ETIL-WCIS; chimera having loop of ETI on the scaffold of WCI; PDB; Protein Data Bank; SDM; site di
Antifreeze activity enhancement by site directed mutagenesis on an antifreeze protein from the beetle Rhagium mordax
Keywords: سایت موتاگنسیس را هدایت می کند; AFP; antifreeze protein; RmAFP1; Rhagium mordax antifreeze protein isoform 1; IBS; ice binding site; IBD; ice binding domain; WT; wild type; CV; column volume; CD; circular dichroism; Antifreeze protein; Site directed mutagenesis; Thermal hysteresis;
Homology modelling, docking, pharmacophore and site directed mutagenesis analysis to identify the critical amino acid residue of PknI from Mycobacterium tuberculosis
Keywords: سایت موتاگنسیس را هدایت می کند; Protein kinases; Homology modelling; Site directed mutagenesis; Substrate; Docking; Pharmacophore analysis
Synthetic peptides derived from the C-terminal 6 kDa region of Plasmodium falciparum SERA5 inhibit the enzyme activity and malaria parasite development
Keywords: سایت موتاگنسیس را هدایت می کند; SERA, serine repeat antigen; SUB1, subtilisin1; hpi, hours post invasion; kDa, kilodaltonPeptide synthesis; Site directed mutagenesis; Enzyme kinetics; Plasmodium
Probing the active site of cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala
Keywords: سایت موتاگنسیس را هدایت می کند; Ll-CCRH1; Leucaena leucocephala cinnamoyl CoA reductase 1; Pg; phenylglyoxal; NAI; N-acetyl imidazole; CA; citraconic acid; PMSF; phenylmethylsulfonyl fluoride; DEPC; diethylpyrocarbonate; WRK; Woodward's reagent K; NEM; N-ethylmaleimide; pHMB; p-hydroxym
Amino acid substitutions of Na,K-ATPase conferring decreased sensitivity to cardenolides in insects compared to mammals
Keywords: سایت موتاگنسیس را هدایت می کند; Na,K-ATPase; Cardenolide-adapted insects; Decreased sensitivity to ouabain; Site directed mutagenesis; Baculovirus expression; Sf9 cells;
A single amino acid determines position specificity of an Arabidopsis thaliana CCoAOMT-like O-methyltransferase
Keywords: سایت موتاگنسیس را هدایت می کند; Flavanone; Methyltransferase; Molecular modelling; Site directed mutagenesis; Arabidopsis thaliana;
Microbial acid-stable α-amylases: Characteristics, genetic engineering and applications
Keywords: سایت موتاگنسیس را هدایت می کند; Starch saccharification; α-Amylase; Acid-stable; Site directed mutagenesis; Directed evolution;
The contribution of two disulfide bonds in the trypsin binding domain of horsegram (Dolichos biflorus) Bowman-Birk inhibitor to thermal stability and functionality
Keywords: سایت موتاگنسیس را هدایت می کند; Trypsin inhibitor; Site directed mutagenesis; Proteolytic stability; Orthogonal geometry; Differential scanning calorimetry;
Asparagine 42 of the conserved endo-inulinase INU2 motif WMNDPN from Aspergillus ficuum plays a role in activity specificity
Keywords: سایت موتاگنسیس را هدایت می کند; Endo-inuinase; Site directed mutagenesis; N42G mutant; Activity modification
Mutation of active site residues Asn67 to Ile, Gln92 to Val and Leu204 to Ser in human carbonic anhydrase II: Influences on the catalytic activity and affinity for inhibitors
Keywords: سایت موتاگنسیس را هدایت می کند; Carbonic anhydrase; Site directed mutagenesis; Active site; Sulfonamide; Sulfamate; Enzyme inhibitor; Inhibition mechanism;
Mapping the Anopheles gambiae Odorant Binding Protein 1 (AgamOBP1) using modeling techniques, site directed mutagenesis, circular dichroism and ligand binding assays
Keywords: سایت موتاگنسیس را هدایت می کند; Odorant Binding Protein; Anopheles gambiae; Circular dichroism spectroscopy; Fluorescence spectroscopy; Site directed mutagenesis; 3D modeling
From in silico to in vitro: Modelling and production of Trichoderma reesei endoglucanase 1 and its mutant in Pichia pastoris
Keywords: سایت موتاگنسیس را هدایت می کند; Cellulase; Endoglucanase; Molecular modelling; Site directed mutagenesis; Trichoderma reesei; Pichia pastoris
Glu-108 is essential for subunit assembly and dimer stability of d-phosphoglycerate dehydrogenase from Entamoeba histolytica
Keywords: سایت موتاگنسیس را هدایت می کند; EhPGDH; Entamoeba histolyticad-phosphoglycerate dehydrogenase; SEC; size exclusion chromatography; Subunit assembly; Electrostatic interactions; Site directed mutagenesis; Molecular dynamic simulations; pH;
Evidence regarding the hypothesis that the histidine-histidine contact pairs may affect protein stability
Keywords: سایت موتاگنسیس را هدایت می کند; Bacillus amyloliquefaciens α-amylase; Histidine; Ca-III binding site; Site directed mutagenesis; Thermal stability;
Probing the role of asparagine mutation in thermostability of Bacillus KR-8104 α-amylase
Keywords: سایت موتاگنسیس را هدایت می کند; Bacillus α-amylase; Site directed mutagenesis; Thermostability
Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism
Keywords: سایت موتاگنسیس را هدایت می کند; White spruce; Picea glauca; Pinaceae; Gibberellins; Diterpene resin acid; Abietadiene; Site directed mutagenesis; Terpene synthase fold; Conifer defense;
The N-terminal β-barrel domain of mammalian lipoxygenases including mouse 5-lipoxygenase is not essential for catalytic activity and membrane binding but exhibits regulatory functions
Keywords: سایت موتاگنسیس را هدایت می کند; Eicosanoids; Lipid peroxidation; Molecular enzymology; Structural biology; Site directed mutagenesis; Reaction specificity
The activity of a designer tissue inhibitor of metalloproteinases (TIMP)-1 against native membrane type 1 matrix metalloproteinase (MT1-MMP) in a cell-based environment
Keywords: سایت موتاگنسیس را هدایت می کند; N-TIMP-1mt1 (V4A + P6V + T98L), N-terminal domain of tissue inhibitor of metalloproteinases-1 (TIMP-1) engineered to inhibit membrane type 1 matrix metalloproteinase (MT1-MMP)TIMP-1; MT1-MMP; Site directed mutagenesis; Cell-based assays; Cancer therapeuti
A quantitative NMR spectroscopic examination of the flexibility of the C-terminal extensions of the molecular chaperones, αA- and αB-crystallin
Keywords: سایت موتاگنسیس را هدایت می کند; small heat-shock protein; molecular chaperone; NMR Spectroscopy; C-terminal extension; site directed mutagenesis; DTT; dithiothreitol; HSQC; Heteronuclear Single Quantum Coherence; IPTG; isopropyl-1-thio-β-d-galactopyranoside; LB; Luria-Bertani; NMR; Nuc
Structure-function analysis of the highly conserved charged residues of the membrane protein FT1, the main folic acid transporter of the protozoan parasite Leishmania
Keywords: سایت موتاگنسیس را هدایت می کند; FA; folic acid; FBT; Folate Biopterin Transporter; MTX; methotrexate; NHS; N-hydroxysuccinimide; TMS; trans membrane segments; Folate transporters; Methotrexate; Site directed mutagenesis; Leishmania; Transport studies;
Modification of the heme active site to increase the peroxidase activity of thermophilic cytochrome P450: A rational approach
Keywords: سایت موتاگنسیس را هدایت می کند; Peroxidase activity; Rational protein design; Thermus thermophilus; CYP175A1; Heme active site; Site directed mutagenesis
Mechanism of direct bicarbonate transport by the CFTR anion channel
Keywords: سایت موتاگنسیس را هدایت می کند; Bicarbonate; CFTR; CF mutation; Permeation; Site directed mutagenesis; Electrophysiology
Distal end of 105–125 loop – A putative reductase binding domain of phthalate dioxygenase
Keywords: سایت موتاگنسیس را هدایت می کند; Rieske center; Iron–sulfur center; Dioxygenase system; Reductase; Phthalate; Site directed mutagenesis; Electron transfer
Improved hydrogen production by uptake hydrogenase deficient mutant strain of Rhodobacter sphaeroides O.U.001
Keywords: سایت موتاگنسیس را هدایت می کند; Rhodobacter sphaeroides; Biohydrogen; Uptake hydrogenase; Site directed mutagenesis; Genetic modification
Structural integrity of the Beta Beta Alpha-Metal finger motif is required for DNA binding and stable protein–DNA complex formation in R.KpnI
Keywords: سایت موتاگنسیس را هدایت می کند; Restriction endonuclease; R.KpnI; ββα-Me finger; HNH Motiff; Site directed mutagenesis; Protein DNA complex
Importance of individual amino acids in the Switch I region in eEF2 studied by functional complementation in S. cerevisiae
Keywords: سایت موتاگنسیس را هدایت می کند; Elongation factor 2; Functional complementation; Site directed mutagenesis; Switch I; Yeast; aEF; archaebacterial elongation factor; eEF; eukaryotic elongation factor; EF; eubacterial elongation factor; GAP; GTPase activating protein; GAC; GTPase activati
Molecular mechanism of arachidonic acid inhibition of the CFTR chloride channel
Keywords: سایت موتاگنسیس را هدایت می کند; Arachidonic acid; Blocker binding site; Chloride channel; Open channel block; Site directed mutagenesis