
Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
Keywords: CCS; countercurrent separation; TLC; thin-layer chromatography; K; partition coefficient; Rf; retention factor for TLC; SS; solvent system; HEMWat; hexanes-ethyl acetate-methanol-water biphasic solvent system; ChMWat; chloroform-methanol-water biphasic so