کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7608150 1493373 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
چکیده انگلیسی
Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1553, 8 June 2018, Pages 108-115
نویسندگان
, , , , , , ,