Keywords: MTT; 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide; DMSO; dimethylsulphoxide; TLCK; Nα-Tosyl-l-lysine chloromethyl ketone hydrochloride; HepG2; Apoptosis; Lawsone; Metallic complexes; Cytotoxicity;
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Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses
Keywords: ABTS; 2,2â²-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid; ALA; α-linolenic acid; BHT; butylated hydroxytoluene; CE; catechin; CFPP; cold-filter plugging point; DHA; docosahexaenoic acid; DPPH; 2,2-diphenyl-1-picrylhydrazyl; EPA; eicosapentaenoic a
Digestive enzymes of the Californian two-spot octopus, Octopus bimaculoides (Pickford and McConnaughey, 1949)
Keywords: DG; digestive gland; EDTA; ethylenediaminetetraacetic acid; GJ; crop gastric juices; PEPST A; Pepstatin A; Phe; phenantroline; PMSF; phenylmethylsulfonyl fluoride; SG; salivary glands; SIT; soybean trypsin inhibitor; TLCK; Tosyl-L-lysyl-chloromethane hydr
W1038 near D-loop of NBD2 is a focal point for inter-domain communication in multidrug transporter Cdr1 of Candida albicans
Keywords: ABC; ATP-binding cassette; TMD; transmembrane domain; NBD; nucleotide binding domain; TMH; transmembrane helix; ICL; intracellular loop; Cdr1p; Candida drug resistance 1 protein; Pdr5p; pleiotropic drug resistance 5 protein; TAP; transporter associated wi
DmCatD, a cathepsin D-like peptidase of the hematophagous insect Dipetalogaster maxima (Hemiptera: Reduviidae): Purification, bioinformatic analyses and the significance of its interaction with lipophorin in the internalization by developing oocytes
Keywords: β-ATPase; β-chain of ATP synthase; OCT; Optimal Cutting Temperature; BSA; Bovine serum albumin; DMP; dimethylpimelimidate; FBS; fetal bovine serum; PMSF; phenylmethyl-sulfonyl fluoride; TLCK; N-α-p-tosyl-L-lysine chloromethyl ketone; PBS; phosphate buf
The gene product of a Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase regulatory subunit is a monomeric protein that is not capable of binding cyclic nucleotides
Keywords: Trypanosoma equiperdum; Regulatory subunits of the cAMP-dependent protein kinase; Gene cloning; Protein expression in bacteria; Protein purification; Biochemical characterization; Dourine; Trypanosomatid parasites; PKA; cAMP-dependent protein kinase; R; P
Full length articleTranscript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum
Keywords: Plasmodium falciparum; Proteases; Schizont; Gametocyte; Transmission; Drug target; EGF; epidermal growth factor-like domain; GPI; Glycosylphosphatidylinositol; TLCK; Tosyl-L-lysyl-chloromethane hydrochloride; TPCK; Tosyl phenylalanyl chloromethyl ketone;
Recombinant expression, refolding, purification and characterization of Pseudomonas aeruginosa protease IV in Escherichia coli
Keywords: Pseudomonas aeruginosa; Protease IV; Recombinant expression; Refolding; Purification; Activity; TRX; thioredoxin; LB medium; Luria-Bertani medium; ORF; open reading frame; DO; dissolved oxygen; FCM; fermentation complex medium; IPTG; isopropyl thio-β-d-g
Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression
Keywords: CanPIs; Capsicum annuum proteinase inhibitors; BApNA; N-α-benzoyl-DL-arginine p-nitroanilide; TPCK; Na-tosyl-l-phenyl alanine chloromethyl ketone; TLCK; Na-p-tosyl-l-lysine chloromethyl ketone; IRD; Inhibitor Repeat Domain; PMSF; Phenyl methyl sulfonyl f
Bowman-Birk proteinase inhibitor from Clitoria fairchildiana seeds: Isolation, biochemical properties and insecticidal potential
Keywords: BBI; Bowman-Birk inhibitor; CD; circular dichroism; DTT; dithiothreitol; RP-HPLC; reversed-phase high performance liquid chromatography; Ki; inhibition constant; MALDI-TOF; matrix assisted laser desorption ionisation time of flight mass spectrometry; PI
Lysine acetylation is a common post-translational modification of key metabolic pathway enzymes of the anaerobe Porphyromonas gingivalis
Keywords: HBA; horse blood agar; BHI; brain heart infusion; TLCK; Nα-tosyl-l-lysine chloromethyl ketone hydrochloride; TCA; Trichloroacetic acid; PBST; PBS with 0.05% Tween 20; Lysine acetylation; Anaerobe; Catabolism; Immune-affinity enrichment; Mass spectrometry
G protein-membrane interactions I: Gαi1 myristoyl and palmitoyl modifications in protein-lipid interactions and its implications in membrane microdomain localization
Keywords: AcNPV; Autographa californica nuclear polyhedrosis virus; AMF; 30 μM AlCl3, 50 mM MgCl2 and 10 mM NaF; Biotin-HPDP; N-[6-(biotinamido)hexyl]-3â²-(2â²-pyridyldithio) propionamide; C12E10; polyoxyethylene 10-lauryl ether; CHO; cholesterol; GPCR; G pro
Modification of p115RhoGEF Ser330 regulates its RhoGEF activity
Keywords: ATRA; All-trans retinoic acid; DH; Dbl homology; DMEM; Dulbecco's modified Eagle's medium; FBS; Fetal bovine serum; GAP; GTPase activating protein; HPLC-MS/MS; High pressure/tandem mass spectrometry; LARG; Leukemia-associated RhoGEF; LC/MS/MS; Liquid ch
Coagulant thrombin-like enzyme (barnettobin) from Bothrops barnetti venom: Molecular sequence analysis of its cDNA and biochemical properties
Keywords: Thrombin-like enzyme; Deglycosylation; Bothrops barnetti; Barnettobin; Animal venoms; Bb-TLE; thrombin-like enzyme from Bothrops barnetti; DL-BAPNA; α-N-benzoyl-DL-arginine-p-nitroanilide; pNA; p-nitroanilide; TAME; Nα-tosyl-L-Arg-methyl ester; SBTI; so
Insensitive trypsins are differentially transcribed during Spodoptera frugiperda adaptation against plant protease inhibitors
Keywords: BAPNA, N-Benzoyl-dl-arginine p-nitroanilide; EATI, Entada acaciifolia trypsin inhibitor; qRT-PCR, quantitative real time PCR; SPI, soybean Bowman-Birk inhibitor; SKTI, soybean Kunitz inhibitor; Suc-AAPF-PNA, N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide; TLCK
Inhibitory effects of a Kunitz-type inhibitor from Pithecellobium dumosum (Benth) seeds against insect-pests' digestive proteinases
Keywords: Pithecellobium dumosum; Trypsin inhibitor; Kunitz; Insect proteinase; Plant-defense; HPLC; high performance liquid chromatography; TCA; trichloroacetic acid; Ki; inhibition constant; TFA; trifluoroacetic acid; ACN; acetonitrile; BApNA; N-α-benzoyl-dl-arg
Leishmania (L.) amazonensis peptidase activities inside the living cells and in their lysates
Keywords: DTT; 1,4-dithiothreitol; MCA; [7-amino-4-methyl] coumarin; E64; 1-[l-N-(trans-epoxysuccinyl)leucyl]amino-4-guanidinobutane; Abz; ortho-aminobenzoic acid; EDDnp[; N-(2,4-dinitrophenyl) ethylenediamine]; TLCK; tosyl-lysine chloromethyl ketone; FRET; fluores
Chymotrypsin-like serine proteinases are involved in the maintenance of cell viability
Keywords: Chymotrypsin; Proteinases; Apoptosis; Serine; Inhibitor; Diphenylphosphonate; PARP; Poly (ADP-ribose) polymerase; PS; phosphatidylserine; TMR-PheP(OPh)2; N-α-tetramethylrhodamine phenylalanyl diphenylphosphonate; TLCK; N-α-tosyl-l-lysine chloromethylket
Differential mode of attack on membrane phospholipids by an acidic phospholipase A2 (RVVA-PLA2-I) from Daboia russelli venom
Keywords: pBPB; p-bromophenacyl bromide; DTT; Dithiothreitol; FA; Fatty acid; IAA; Iodoacetamide; PC; Phosphatidylcholine; PE; Phosphatidylethanolamine; PS; Phosphatidylserine; RVV; Russell's viper venom; TLCK; N-α-p-tosyl-l-lysine chloromethyl ketone; TPCK; Tosyl
The peptidases of Trypanosoma cruzi: Digestive enzymes, virulence factors, and mediators of autophagy and programmed cell death
Keywords: CPs; cysteine proteinases; SPs; serine proteinases; MPs; metalloproteinases; APs; aspartyl proteinases; 20S and 26S proteasome: proteasome oligomers with a sedimentation coefficient of 20S and 26S; respectively; Z; N-benzyloxycarbonyl; NHMec; amidomethyl
Effect of divalent cations on the porcine kidney cortex membrane-bound form of dipeptidyl peptidase IV
Keywords: DPP-IV; dipeptidyl peptidase IV; Xaa; any common aminoacids; GIP; gastric inhibitory peptide; GLP-1; glucagon-like peptide 1; aas; aminoacids; BPTI; bovine pancreatic trypsin inhibitor; SbTI; soybean trypsin inhibitor; TLCK; tosyl-l-lysine chloromethyl ke
Free heme is a danger signal inducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts
Keywords: CCRC; cultured cardiac resident cells; CRC; cardiac resident cells; EAM; experimental autoimmune myocarditis; HO-1; heme oxygenase-1; IL-1; interleukin-1; Lcn2; lipocalin-2; ROS; reactive oxygen species; RASMC; rat aortic smooth muscle cells; RCF; rat car
Inhibition of human erythrocyte invasion by Babesia divergens using serine protease inhibitors
Keywords: RBCs; red blood cells; E64; trans-epoxysuccinyl-l-leucylamido-(4-guanidinio)butane]; AEBSF; 4-(2-aminoethyl)-benzenosulfonyl fluoride; EGTA; ethylene glycol bis (β-aminoethylether)-N,N,N,N-tetraacetic acid; 3,4-DCI; 3,4-dichloroisocoumarin; PMSF; phenylm
Inhibition of the CaaX proteases Rce1p and Ste24p by peptidyl (acyloxy)methyl ketones
Keywords: Z; benzyloxycarbonyl; AOMK; (acyloxy)methyl ketone; DMSO; dimethyl sulfoxide; EDTA; ethylenediaminetetraacetic acid; E64; N-[N-(l-3-trans-carboxyoxiran-2-carbonyl)-l-leucyl]agmatine; ICMT; isoprenylcysteine carboxyl methyltransferase; TLCK; tosyl-l-lysyl-
Use of the myosin motor domain as large-affinity tag for the expression and purification of proteins in Dictyostelium discoideum
Keywords: ARP1; actin-related protein 1; ARP11; actin-related protein 11; N-CBZPA; N-carbobenzyloxy-phenyl-alanine; Dd; Dictyostelium discoideum; DynHC; dynein heavy chain; DynIC; dynein intermediate chain; DynLIC; dynein light-intermediate chain; GST; Glutathion
Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development
Keywords: Plasmodium berghei; Malaria; Parasite; Gametogenesis; Gametes; Zygotes; Exflagellation centers; Leupeptin; PMSF; phenylmethylsulfonyl fluoride; TLCK; N-α-p-tosyl-l-lysine chloromethyl ketone; TPCK; l-1-tosylamide-2-phenylethyl-chloromethyl ketone; 1,10-p
A novel protease from Entamoeba histolytica homologous to members of the family S28 of serine proteases
Keywords: DPP; dipeptidyl peptidase; PRCP; prolylcarboxypeptidase; Suc-AAF-AMC; N-succinyl-l-Ala-l-Ala-l-Phe-7-amido-4-methylcoumarin; DFP; di-isopropyl fluorophosphate; E-64; 1-trans-epoxysuccinyl-l-leucilamide-(4-guanidine)-butane; EDTA; ethylenediaminetetraaceti
Leucine aminopeptidase from etiolated barley seedlings: characterization and partial purification of isoforms
Keywords: antipain; [(S)-1-carboxy-2-phenyl]-carbamoyl-arg-val-arginal); DEAE; diethylaminoethyl; E-64; trans-epoxysuccinyl-l-leucylamido-(4-guanidino) butane; LAP; leucine aminopeptidase; Leu-MCA; L-leucine 4-methyl-coumaryl-7-amide; Leu-pNA; L-Leucine p-nitroanil
Production of protease from a new alkalophilic Bacillus sp. I-312 grown on soybean meal: optimization and some properties
Keywords: EDTA; ethylenediaminetetraacetic acid; LBTI; limabean trypsin inhibitor; PMSF; phenylmethylsulfonyl fluoride; SBM; soybean meal; SBTI; soybean trypsin inhibitor; SDS; sodium dodecyl; sulphate; TCA; trichloroacetic acid; TLCK; N-α-tosyl-l-lysine chloromet
Characterization of arazyme, an exocellular metalloprotease isolated from Serratia proteamaculans culture medium
Keywords: Abz; ortho-aminobenzoic acid; Cbz; carbobenzyl; DMSO; dimethyl sulphoxide; EDDnp; N-(2,4-dinitrophenyl)ethylenediamine; EDTA; ethylenediaminetetraacetic acid; E-64; trans-epoxy-succinyl-l-leucylamido-(4-guanido)-butene; FPLC; fast protein liquid chromatog
A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae
Keywords: Chymotrypsin; Digestive proteinase; Midgut; Tenebrio molitor; Yellow mealworm; Ac; acetyl; AM; anterior midgut; Bz; benzoyl; DMF; dimethyl formamide; E-64; l-trans-epoxysuccinyl-l-leucylamido(4-guanidino) butane; Glp; pyroglutamyl; PM; posterior midgut; P
Specific human antibodies do not inhibit Trypanosoma cruzi oligopeptidase B and cathepsin B, and immunoglobulin G enhances the activity of trypomastigote-secreted oligopeptidase B
Keywords: Trypanosoma cruzi; Cathepsin B; Oligopeptidase B; Enzymatic activity; AMC; 7-amido-4-methylcoumarin; DEAE; diethylaminoethyl-sephacel; E-64; trans-epoxysuccinyl-l-leucylamido-(4-guanidino)butane; HAS; human serum albumin; HPLC; high-pressure liquid chroma
Evaluation of the neuronal apoptotic pathways involved in cytoskeletal disruption-induced apoptosis
Keywords: AC-DEVD-CHO; Ac-Asp-Glu-Asp-Val-Aldehyde; AC-LEHD-CHO; Ac-Leu-Glu-His-Asp-aldehyde; AIF; apoptosis inducing factor; BAPTA-AM; 1,2-bis-(o-aminophenoxy)-ethane-N,N,-Nâ²,Nâ²-tetraacetic acid tetraacetoxy-methyl ester; CGNs; cerebellar granule neurons; DPQ;
The antigenic and catalytically active formamidase of Paracoccidioides brasiliensis: protein characterization, cDNA and gene cloning, heterologous expression and functional analysis of the recombinant protein
Keywords: Paracoccidioides brasiliensis; Serum reactive protein; formamidase; Immunological reactivity; Catalytic activity; BCIP; 5-bromo-4-chloro-3-indolyl phosphate; CTAB; cationic hexadecyl trimethyl ammonium bromide; DTT; dithiotreitol; EDTA; ethylenediaminetet
Rubrerythrin and peroxiredoxin: Two novel putative peroxidases in the hydrogenosomes of the microaerophilic protozoon Trichomonas vaginalis
Keywords: ASB-14; amidosulfobetaine-14; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; DTT; dithiothreitol; MOPS; 3-(N-morpholino)propanesulfonic acid; TLCK; tosyl lysyl chloromethyl ketone; Hydrogenosome; Oxidative stress; Peroxidase; Prokaryoti
Natterins, a new class of proteins with kininogenase activity characterized from Thalassophryne nattereri fish venom
Keywords: Kininogenase; Tissue-kallikrein; Natterin; Fish venom; Thalassophryne nattereri; EDDnp; N-[2,4-dinitrophenyl]-etilenedianine; EDTA; ethylenediaminetetraacetic acid; ESTs; expressed sequence tags; hK1; human kallikrein 1; PMSF; phenyl methyl sulfonyl fluor
Snake venom serine proteinases: sequence homology vs. substrate specificity, a paradox to be solved
Keywords: Snake venom serine proteinases; Blood clotting; Platelet-aggregation; Kinin-release; Protein-C activation; Factor V activation; Molecular recognition; Molecular modeling; 3D structure-activity relationships; ε-ACA; ε-aminocaproic acid; ADP; adenosine di