کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318857 | 1539120 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digestive enzymes of the Californian two-spot octopus, Octopus bimaculoides (Pickford and McConnaughey, 1949)
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کلمات کلیدی
PMSFPepstatin APhenantrolineTLCKTPCKSITPHEAmylases - آمیلاEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدTosyl phenylalanyl chloromethyl ketone - تسیل فنیل آلانیل کلرومتیل کتونTemperature - دماSalivary glands - غدد بزاقیDigestive gland - غدد گوارشیphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدLipases - لیپازهاInhibitors - مهارکنندهsoybean trypsin inhibitor - مهارکننده تریپسین سویاProteases - پروتئازDivalent ions - یونهای دوتایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Octopus bimaculoides is an important commercially fished species in the California Peninsula with aquaculture potential; however, to date limited information is available regarding its digestive physiology. The objective of this study was focused on biochemically characterizing the main enzymes involved in the digestion of O. bimaculoides. Optimum pH, temperature and thermostability were determined for amylases, lipases, trypsin and chymotrypsin; optimum pH and protease inhibitor effect were assessed for acidic and alkaline proteases, and the effect of divalent ions on trypsin and chymotrypsin activity was evaluated in enzymatic extracts from the digestive (DG) and salivary glands (SG) and crop gastric juices (GJ). High amylase activity was detected in GD and GJ whereas this activity is very low in other cephalopods. Salivary glands had the greatest activity in most of the enzyme groups, showing the importance of this organ in digestion. Optimum pH was different depending on the organ and enzyme analyzed. The optimum pH in DG was 3 showing the predominance of acidic proteases in the digestion process. All enzymes were resistant and stable at high temperatures in contrast with other marine species. Trypsin and chymotrypsin activity were highly incremented with the presence of Mg2Â +, Co2Â +, Cu2Â + and Zn2Â + in some tissues. The inhibitor assay showed the importance of serine proteases, metalloproteases and aspartic proteases in the digestive process of this species. This study is the first in assessing the main digestive enzymes of O. bimaculoides and in remarking the importance of other digestive enzyme groups besides proteases in octopuses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 215, January 2018, Pages 10-18
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 215, January 2018, Pages 10-18
نویسندگان
Laura Elizabeth Ibarra-GarcÃa, Dariel Tovar-RamÃrez, Carlos Rosas, Ángel Isidro Campa-Córdova, José Manuel Mazón-Suástegui,