Keywords: ANSES; French Agency for Food, Environmental and Occupational Health and Safety; AUC; area under the curve; CFSE; carboxyfluorescein succinimidyl ester; EMEM; Eagle's minimum essential medium; FBS; fetal bovine serum; HA; hemagglutinin; HAU; hemagglutin
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Keywords: MMPs; Matrix metalloproteinases; ECM; extracellular matrix; TSP-2; Thrombospondin-2; FAK; focal adhesion kinase; Akt; protein kinase B; NF-κB; nuclear factor kappa B; Ig; immunoglobulin; DN; dominant negative; shRNA; short hairpin RNA; TCGA; The Cancer G
Keywords: MS; mass spectrometry; LC; liquid chromatography; MRM; multiple reaction monitoring; DP; declustering potential; CE; collision energy; apoA1; apolioprotein A-1; RBP4; retinol-binding protein 4; TTR; transthyretin/prealbumin; CO9; complement component 9; C
Keywords: α-A-NP; anti-influenza A nucleoprotein; BSA; bovine serum albumin; C+; normalized concentration; CCD; charge-coupled device; D+; normalized location across the micromixer; DI; deionized; DMEM; Dulbecco's modified Eagle's medium; ELISA; enzyme-linked immu
Keywords: EP; endopeptidase; PAGE; polyacrylamide gel electrophoresis; SDS; sodium dodecylsulfate; DMSO; dimethyl sulfoxide; DTT; dithiothreitol; AEBSF; 4-(2-aminoethyl) benzene sulfonyl fluoride hydrochloride; PMSF; phenylmethanesulfonyl fluoride; EGTA; ethylene g
Keywords: ACF; aberrant crypt foci; AMC; 7-amino-4-methyl coumarin; Akt1; v-akt murine thymoma viral oncogene homolog 1; ATCC; American Type Culture Collection; CCL; culture clone; CHO; Chinese hamster ovary cells; CPA; cyclopiazonic acid; D9R; nona-d-arginine; DME
Chestnut (Castanea sativa Mill.) industrial wastes as a valued bioresource for the production of active ingredients
Keywords: AA; ascorbic acid; AQP3; aquaporin-3; BHT; butylhydroxytoluene; C; catechin; CAE; caffeic acid equivalents; CE; catechin equivalents; DE; dry extract; DMEM; dulbecco's modified eagle's medium; DPPH; 2,2-diphenyl-1-picrylhydrazyl; FBS; fetal bovine ser
Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses
Keywords: ABTS; 2,2â²-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid; ALA; α-linolenic acid; BHT; butylated hydroxytoluene; CE; catechin; CFPP; cold-filter plugging point; DHA; docosahexaenoic acid; DPPH; 2,2-diphenyl-1-picrylhydrazyl; EPA; eicosapentaenoic a
Digestive enzymes of the Californian two-spot octopus, Octopus bimaculoides (Pickford and McConnaughey, 1949)
Keywords: DG; digestive gland; EDTA; ethylenediaminetetraacetic acid; GJ; crop gastric juices; PEPST A; Pepstatin A; Phe; phenantroline; PMSF; phenylmethylsulfonyl fluoride; SG; salivary glands; SIT; soybean trypsin inhibitor; TLCK; Tosyl-L-lysyl-chloromethane hydr
W1038 near D-loop of NBD2 is a focal point for inter-domain communication in multidrug transporter Cdr1 of Candida albicans
Keywords: ABC; ATP-binding cassette; TMD; transmembrane domain; NBD; nucleotide binding domain; TMH; transmembrane helix; ICL; intracellular loop; Cdr1p; Candida drug resistance 1 protein; Pdr5p; pleiotropic drug resistance 5 protein; TAP; transporter associated wi
The gene product of a Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase regulatory subunit is a monomeric protein that is not capable of binding cyclic nucleotides
Keywords: Trypanosoma equiperdum; Regulatory subunits of the cAMP-dependent protein kinase; Gene cloning; Protein expression in bacteria; Protein purification; Biochemical characterization; Dourine; Trypanosomatid parasites; PKA; cAMP-dependent protein kinase; R; P
Biochemical characterization of a novel fibrinolytic enzyme from Cordyceps militaris
Keywords: BSA; bovine serum albumin; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; EDTA; ethylenediamine tetracetic acid; TPCK; N-tosyl-l-phenylalanine chloromethyl ketone; SBTI; soybean trypsin inhibitor; PMSF; phenylmethane sulfonyl fluorid
Some properties of three αB-crystallin mutants carrying point substitutions in the C-terminal domain and associated with congenital diseases
Keywords: αB-Crystallin; Small heat shock proteins; Heterooligomers; Chaperone-like activity; Congenital human diseases; DTT; dithiothreitol; DLS; dynamic light scattering; PMSF; phenylmethane sulfonylfluoride; SDS; sodium dodecyl sulfate; SEC; size exclusion chro
Full length articleTranscript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum
Keywords: Plasmodium falciparum; Proteases; Schizont; Gametocyte; Transmission; Drug target; EGF; epidermal growth factor-like domain; GPI; Glycosylphosphatidylinositol; TLCK; Tosyl-L-lysyl-chloromethane hydrochloride; TPCK; Tosyl phenylalanyl chloromethyl ketone;
M2SR, a novel live single replication influenza virus vaccine, provides effective heterosubtypic protection in mice
Keywords: ADCC; antibody-dependent cellular cytotoxicity; Aichi; influenza A/Aichi/2/1968; APC; allophycocyanin; BAL; bronchoalveolar lavage; BSA; bovine serum albumin; CPE; cytopathic effect; FCS; fetal calf serum; FITC; fluorescein isothiocyanate; HAI; hemaggluti
Clinical peptide and protein quantification by mass spectrometry (MS)
Keywords: Peptide; Protein; Quantitation; Clinical; Laboratory-testing; Mass spectrometry; Immunoassay limitations; Isoforms; Fragments; Assay-harmonization; 25-OH-D; 25-hydroxy Vitamin D; AA; African American; AIC; analyte immunocapture; aa; Amino acid(s); AngI; a
Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression
Keywords: CanPIs; Capsicum annuum proteinase inhibitors; BApNA; N-α-benzoyl-DL-arginine p-nitroanilide; TPCK; Na-tosyl-l-phenyl alanine chloromethyl ketone; TLCK; Na-p-tosyl-l-lysine chloromethyl ketone; IRD; Inhibitor Repeat Domain; PMSF; Phenyl methyl sulfonyl f
Purification and characterization of a fibrinolytic enzyme from the food-grade fungus, Neurospora sitophila
Keywords: BSA; bovine serum albumin; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; EDTA; ethylenediamine tetracetic acid; TPCK; N-tosyl-l-phenylalanine chloromethyl ketone; SBTI; soybean trypsin inhibitor; PMSF; phenylmethane sulfonyl fluorid
Epoxide based inhibitors of the hepatitis C virus non-structural 2 autoprotease
Keywords: HCV; hepatitis C virus; NS; non-structural; SGR; subgenomic replicon; TPCK; tosyl phenylalanine chloromethyl ketone; GdnHCl; guanidine hydrochloride; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate; DTT; dithiothreitol; JFH1; Japanese Fu
Biochemical characterisation of chymotrypsin from the midgut gland of yellowleg shrimp, Penaeus californiensis
Keywords: BSA; bovine serum albumin; DTT; dithiothreitol; pI; isoelectric point; PMSF; phenylmethanesulphonyl fluoride; SAAPFNA; N-succinyl-l-alanyl-l-alanyl-l-prolyl-l-phenylalanine 4-nitroanilide; SBTI; soybean trypsin inhibitor; SDS-PAGE; sodium dodecyl sulpha
G protein-membrane interactions I: Gαi1 myristoyl and palmitoyl modifications in protein-lipid interactions and its implications in membrane microdomain localization
Keywords: AcNPV; Autographa californica nuclear polyhedrosis virus; AMF; 30 μM AlCl3, 50 mM MgCl2 and 10 mM NaF; Biotin-HPDP; N-[6-(biotinamido)hexyl]-3â²-(2â²-pyridyldithio) propionamide; C12E10; polyoxyethylene 10-lauryl ether; CHO; cholesterol; GPCR; G pro
Assessment of naturally occurring covalent and total dimer levels in human IgG1 and IgG2
Keywords: DTT; dithiothreitol; EDTA; ethylene diamine tetraacetic acid; GFB; glufibrinopeptide B; HC; heavy chain; HL; half molecule; LC; light chain; LC/MS; liquid chromatography/mass spectrometry; NEM; N-ethylmaleimide; SDS-PAGE; sodium dodecyl sulfate-polyacryla
Hormonogenic donor Tyr2522 of bovine thyroglobulin. Insight into preferential T3 formation at thyroglobulin carboxyl terminus at low iodination level
Keywords: AChE; acetylcholinesterase; bTg; bovine thyroglobulin; CAD/MS; collisionally activated dissociation mass spectrometry; DABITC; dimethylaminoazobenzene-isothiocyanate; DIT; 3,5-diiodotyrosine; ES/MS; electrospray mass spectrometry; HPLC-ESI-Q-TOF/MS; HPLC
Anti-influenza A virus activity of uridine derivatives of 2-deoxy sugars
Keywords: CC50; concentration of the compound required to reduce cell viability by 50%; CPE; cytopathic effect; CSFV; classical swine fever virus; Ct; cycle threshold; ER; endoplasmic reticulum; HA; hemagglutinin; IC50; concentration of the compound required to red
Modification of p115RhoGEF Ser330 regulates its RhoGEF activity
Keywords: ATRA; All-trans retinoic acid; DH; Dbl homology; DMEM; Dulbecco's modified Eagle's medium; FBS; Fetal bovine serum; GAP; GTPase activating protein; HPLC-MS/MS; High pressure/tandem mass spectrometry; LARG; Leukemia-associated RhoGEF; LC/MS/MS; Liquid ch
The JNK/NFκB pathway is required to activate murine lymphocytes induced by a sulfated polysaccharide from Ecklonia cava
Keywords: CFSE; carboxy fluorescein diacetate succinimidyl ester; Con A; concanavalin A; E. cava; Ecklonia cava; ERK; extracellular signal-regulated kinases; DMSO; dimethylsulfoxide; ELISA; enzyme linked immunosorbent assay; EMSA; electrophoretic mobility shift ass
Chemopreventive and antioxidant activity of 6-substituted imidazo[2,1-b]thiazoles
Keywords: Antioxidants; Chemopreventive agents; Imidazo[2,1-b]thiazoles; Polyphenols; ROS; reactive oxygen species; RNS; reactive nitrogen species; TNF; tumor necrosis factor; NFκB; nuclear factor kappa beta; QR1, NAD(P)H; quinone reductase 1; RXR; retinoid X rece
Physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathies
Keywords: Small heat shock proteins; Oligomeric structure; Chaperone-like activity; Protein deposit diseases; Neuropathies; ACD; α-crystallin domain; AUC; analytical ultracentrifugation; DLS; dynamic light scattering; DTT; dithiothreitol; ME; β-mercaptoethanol; P
Inhibitory effects of a Kunitz-type inhibitor from Pithecellobium dumosum (Benth) seeds against insect-pests' digestive proteinases
Keywords: Pithecellobium dumosum; Trypsin inhibitor; Kunitz; Insect proteinase; Plant-defense; HPLC; high performance liquid chromatography; TCA; trichloroacetic acid; Ki; inhibition constant; TFA; trifluoroacetic acid; ACN; acetonitrile; BApNA; N-α-benzoyl-dl-arg
TPCK inhibits AGC kinases by direct activation loop adduction at phenylalanine-directed cysteine residues
Keywords: TPCK; N-alpha-tosyl-l-phenylalanyl chloromethyl ketone; PDK1; 3-phosphoinositide dependent protein kinase-1; AGC; related to protein kinase A, protein kinase G and protein kinase C; RSK; ribosomal S6 kinase; S6K; S6 kinase; PKA; protein kinase A; ERK; ext
Chymotrypsin-like serine proteinases are involved in the maintenance of cell viability
Keywords: Chymotrypsin; Proteinases; Apoptosis; Serine; Inhibitor; Diphenylphosphonate; PARP; Poly (ADP-ribose) polymerase; PS; phosphatidylserine; TMR-PheP(OPh)2; N-α-tetramethylrhodamine phenylalanyl diphenylphosphonate; TLCK; N-α-tosyl-l-lysine chloromethylket
Differential mode of attack on membrane phospholipids by an acidic phospholipase A2 (RVVA-PLA2-I) from Daboia russelli venom
Keywords: pBPB; p-bromophenacyl bromide; DTT; Dithiothreitol; FA; Fatty acid; IAA; Iodoacetamide; PC; Phosphatidylcholine; PE; Phosphatidylethanolamine; PS; Phosphatidylserine; RVV; Russell's viper venom; TLCK; N-α-p-tosyl-l-lysine chloromethyl ketone; TPCK; Tosyl
Streptomyces erythraeus trypsin inactivates α1-antitrypsin
Keywords: Bz-Arg-pNA; Nα-benzoyl-l-arginine-p-nitroanilide; BT; bovine trypsin; IPTG; isopropyl β-d-thiogalactoside; MALDI-TOF MS; matrix assisted laser desorption ionization time of flight mass spectrometry; PAGE; polyacrylamide gel electrophoresis; PMSF; phenyl
Experimental autoimmune encephalomyelitis: Association with mutual regulation of RelA (p65)/NF-κB and phospho-IκB in the CNS
Keywords: Experimental autoimmune encephalomyelitis; RelA (p65); Phospho-IκB; TPCK;
Construction of a fully active Cys-less elongation factor Tu: Functional role of conserved cysteine 81
Keywords: aa-tRNA; aminoacyl-tRNA; EF-Tu; elongation factor Tu; FRET; fluorescence resonance energy transfer; sm; single molecule; LB; Luria-Bertani; TPCK; N-tosyl-l-phenylalanylchloromethane; Pi; inorganic phosphate; IPTG; isopropyl-β-d-thiogalactopyranoside; S
Free heme is a danger signal inducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts
Keywords: CCRC; cultured cardiac resident cells; CRC; cardiac resident cells; EAM; experimental autoimmune myocarditis; HO-1; heme oxygenase-1; IL-1; interleukin-1; Lcn2; lipocalin-2; ROS; reactive oxygen species; RASMC; rat aortic smooth muscle cells; RCF; rat car
Rapid LC-MS screening for IgG Fc modifications and allelic variants in blood
Keywords: ADCC; antibody dependent cellular cytotoxicity; CV; coefficient of variation; DTT; dithiothreitol; EDTA; ethylenediaminetetraacetic acid; Fuc; fucose; GlcNac; N-acetylglucosamine; HC; heavy chain; LC; light chain; mAb; monoclonal antibody; PQA; product qu
TPCK targets elements of mitotic spindle and induces cell cycle arrest in prometaphase
Keywords: TPCK; Tubulin; Cell division; Apoptosis
Na-Tosyl-Phe chloromethyl ketone prevents granule movement and mast cell synergistic degranulation elicited by costimulation of antigen and adenosine
Keywords: IgE; Mast cells; FcεRI; Adenosine; Degranulation; TPCK
Inhibition of human erythrocyte invasion by Babesia divergens using serine protease inhibitors
Keywords: RBCs; red blood cells; E64; trans-epoxysuccinyl-l-leucylamido-(4-guanidinio)butane]; AEBSF; 4-(2-aminoethyl)-benzenosulfonyl fluoride; EGTA; ethylene glycol bis (β-aminoethylether)-N,N,N,N-tetraacetic acid; 3,4-DCI; 3,4-dichloroisocoumarin; PMSF; phenylm
Inhibition of the CaaX proteases Rce1p and Ste24p by peptidyl (acyloxy)methyl ketones
Keywords: Z; benzyloxycarbonyl; AOMK; (acyloxy)methyl ketone; DMSO; dimethyl sulfoxide; EDTA; ethylenediaminetetraacetic acid; E64; N-[N-(l-3-trans-carboxyoxiran-2-carbonyl)-l-leucyl]agmatine; ICMT; isoprenylcysteine carboxyl methyltransferase; TLCK; tosyl-l-lysyl-
Use of the myosin motor domain as large-affinity tag for the expression and purification of proteins in Dictyostelium discoideum
Keywords: ARP1; actin-related protein 1; ARP11; actin-related protein 11; N-CBZPA; N-carbobenzyloxy-phenyl-alanine; Dd; Dictyostelium discoideum; DynHC; dynein heavy chain; DynIC; dynein intermediate chain; DynLIC; dynein light-intermediate chain; GST; Glutathion
Novel isoforms of NADPH-oxidase in cerebral vascular control
Keywords: Aβ; amyloid-β peptide; ACE; angiotensin-converting enzyme; ACh; acetylcholine; AD; Alzheimer's disease; AEBSF; aminoethylbenzenesulfono fluoride; APP; amyloid precursor protein; CBF; cerebral blood flow; cGMP; guanosine monophosphate; DETCA; diethyldith
Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development
Keywords: Plasmodium berghei; Malaria; Parasite; Gametogenesis; Gametes; Zygotes; Exflagellation centers; Leupeptin; PMSF; phenylmethylsulfonyl fluoride; TLCK; N-α-p-tosyl-l-lysine chloromethyl ketone; TPCK; l-1-tosylamide-2-phenylethyl-chloromethyl ketone; 1,10-p
A novel protease from Entamoeba histolytica homologous to members of the family S28 of serine proteases
Keywords: DPP; dipeptidyl peptidase; PRCP; prolylcarboxypeptidase; Suc-AAF-AMC; N-succinyl-l-Ala-l-Ala-l-Phe-7-amido-4-methylcoumarin; DFP; di-isopropyl fluorophosphate; E-64; 1-trans-epoxysuccinyl-l-leucilamide-(4-guanidine)-butane; EDTA; ethylenediaminetetraaceti
Leucine aminopeptidase from etiolated barley seedlings: characterization and partial purification of isoforms
Keywords: antipain; [(S)-1-carboxy-2-phenyl]-carbamoyl-arg-val-arginal); DEAE; diethylaminoethyl; E-64; trans-epoxysuccinyl-l-leucylamido-(4-guanidino) butane; LAP; leucine aminopeptidase; Leu-MCA; L-leucine 4-methyl-coumaryl-7-amide; Leu-pNA; L-Leucine p-nitroanil
Production of protease from a new alkalophilic Bacillus sp. I-312 grown on soybean meal: optimization and some properties
Keywords: EDTA; ethylenediaminetetraacetic acid; LBTI; limabean trypsin inhibitor; PMSF; phenylmethylsulfonyl fluoride; SBM; soybean meal; SBTI; soybean trypsin inhibitor; SDS; sodium dodecyl; sulphate; TCA; trichloroacetic acid; TLCK; N-α-tosyl-l-lysine chloromet
Characterization of arazyme, an exocellular metalloprotease isolated from Serratia proteamaculans culture medium
Keywords: Abz; ortho-aminobenzoic acid; Cbz; carbobenzyl; DMSO; dimethyl sulphoxide; EDDnp; N-(2,4-dinitrophenyl)ethylenediamine; EDTA; ethylenediaminetetraacetic acid; E-64; trans-epoxy-succinyl-l-leucylamido-(4-guanido)-butene; FPLC; fast protein liquid chromatog
A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae
Keywords: Chymotrypsin; Digestive proteinase; Midgut; Tenebrio molitor; Yellow mealworm; Ac; acetyl; AM; anterior midgut; Bz; benzoyl; DMF; dimethyl formamide; E-64; l-trans-epoxysuccinyl-l-leucylamido(4-guanidino) butane; Glp; pyroglutamyl; PM; posterior midgut; P
Role of nitric oxide in larval and juvenile fish
Keywords: Ach; acetylcholine; AII; angiotensin II; CD14; pattern recognition receptor; FAD; flavin adenine dinucleotide; FMN; flavin mononucleotide; GMP; guanosine monophosphate; GST; glutathione S-transferase; GTN; glyceryl trinitrate; IFN-γ; interferon-γ; ISDN;