Magnetic nanoparticle-based immunoassay for rapid detection of influenza infections by using an integrated microfluidic system

This study firstly attempt to produce size-tunable magnetic MnFe2O4 nanoparticles, combining with an integrated microfluidic system that involves characterized micro-devices working with nanoparticles for influenza detection with a fluorescence immunoassay. With the characteristics of magnetic nanoparticles, selective control, uniform distribution, bio-compatibility and high surface-to-volume ratio, the sensitivity of FIA is anticipated to advance. By further uniting with surface modification method, the non-specific binding of nanoparticles is reduced and background noise is also diminished. The optical signal of nanoparticles is improved two times stronger than commercial beads, in 20 min. Furthermore, five clinical specimens were tested to verify this developed method.216