کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442617 | 1302567 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development
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کلمات کلیدی
PMSFPlasmodium bergheiTPCKTLCKN-α-p-tosyl-l-lysine chloromethyl ketone1,10-Phenanthroline - 1،10-PhenanthrolineParasite - انگلZygotes - زیتوتاphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدleupeptin - لئوپتینMalaria - مالاریاProtease inhibitor - مهارکننده پروتئازProtease - پروتئازGametes - گامتGametogenesis - گامتوژنز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development](/preview/png/9442617.png)
چکیده انگلیسی
Plasmodium berghei: The effect of five protease inhibitors, TPCK, TLCK, PMSF, leupeptin, and 1,10-phenanthroline on in vitro gametogenesis and early zygote development of P. berghei was investigated. PMSF and leupeptin showed no effect. Cysteine/serine protease inhibitors TPCK/TLCK at concentrations of 75 and 100 μM were effective on inhibiting exflagellation center formation, and this effect was reversible with the addition of l-cysteine. Exflagellation center formation was most effectively blocked by 1,10-phenanthroline (1 mM), and exflagellation center numbers were restored by the addition of Zn2+. A reduction of ookinete production was observed when TPCK/TLCK (100 μM) was added at 2 h after gametogenesis, but no effect was observed with 1,10-phenanthroline (1 mM). Our results suggest that proteolysis is important in both gametocyte activation and sexual development of P. berghei.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 111, Issue 4, December 2005, Pages 255-259
Journal: Experimental Parasitology - Volume 111, Issue 4, December 2005, Pages 255-259
نویسندگان
Jorge A. Torres, Mario H. Rodriguez, Maria C. Rodriguez, Fidel de la Cruz Hernandez-Hernandez,