Keywords: کل فلورسنس بازتاب داخلی; TIRF; total internal reflection fluorescence; pTIRF; polarized total internal reflection fluorescence; SLB; supported lipid bilayer; STuBs; supported tubulated bilayers; COPII; coat protein complex II; SMrT; supported membrane tubules; SUPER; supported me
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Keywords: کل فلورسنس بازتاب داخلی; Biosensor; Surface plasmon resonance; Fluorescence; Bioassay; Grating; Total internal reflection fluorescence; Epifluorescence;
Keywords: کل فلورسنس بازتاب داخلی; CER; ceramide; C9-4HMT-DANA; 9-[4-hydroxymethyl-[1,2,3]triazol-1-yl]-2,3-didehydro-N-acetyl-neuraminic acid; DANA; 2,3-didehydro-N-acetyl-neuraminic acid; ECM; extracellular matrix; FN; fibronectin; hNeu; human neuraminidase enzyme; HPTLC; high performanc
Keywords: کل فلورسنس بازتاب داخلی; PFM; protrusion force microscopy; AFM; atomic force microscopy; TIRF; total internal reflection fluorescence; Podosomes; Macrophages; Multiparticle tracking; Force; Mechanosensing; Collective dynamics;
Keywords: کل فلورسنس بازتاب داخلی; AU; absorbance unit (Chapter 3.1) or arbitrary unit (Figs. 2 and 5); BLM; Bloom's syndrome DNA helicase; bp; base pair; ds; double-stranded; DxSO4; dextran sulfate; FRET; Förster Resonance Energy Transfer; LDH; lactate dehydrogenase; mant; methylanthra
Keywords: کل فلورسنس بازتاب داخلی; AMP; LC-MS; ELISA; ELIMSA; PSA; Antibody; AP-SA; UV-VIS; ultraviolet-visible; AP; alkaline phosphatase; ELISA; enzyme-linked immunosorbent assay; TIRF; total internal reflection fluorescence; LC; liquid chromatography; ESI; electrospray ionization
Keywords: کل فلورسنس بازتاب داخلی; Human pancreatic lipase; Optical sensors; Reflectometric interference spectroscopy; Total internal reflection fluorescence; Fluorescence labeling;
Keywords: کل فلورسنس بازتاب داخلی; ADCC; antibody-dependent cellular cytotoxicity; AQP4; aquaporin-4; BSA; bovine serum albumin; CDC; complement-dependent cytotoxicity; CNS; central nervous system; dSTORM; direct stochastic reconstruction microscopy; EDSS; expanded disability status scale;
Keywords: کل فلورسنس بازتاب داخلی; ÎÏ; transmembrane electric potential difference; BLM; bilayer lipid membrane; BSA; bovine serum albumin; C2R1; Rhodamine 19 ethyl ester or Rhodamine 6G; C4R1; Rhodamine 19 butyl ester; C4R4; Rhodamine B butyl ester; C8R1; Rhodamine 19 octyl ester; C10R1
The spatial molecular pattern of integrin recognition sites and their immobilization to colloidal nanobeads determine α2β1 integrin-dependent platelet activation
Keywords: کل فلورسنس بازتاب داخلی; Platelet signaling; Platelet activation; Integrin α2β1 cluster; Nanopatterning; Biofunctionalized nanobeads and nano-sized membrane protein clusters; BCECF-AM; 2â²,7â²-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-acetoxymethyl ester; BSA; bovine
Atomic force microscopy of adsorbed proteoglycan mimetic nanoparticles: Toward new glycocalyx-mimetic model surfaces
Keywords: کل فلورسنس بازتاب داخلی; AFM; atomic force microscopy; CHI; chitosan; CS; chondroitin sulfate; DLS; dynamic light scattering; FT-SPR; Fourier transform surface plasmon resonance; GAG; glycosaminoglycan; HA; hyaluronan; HEP; heparin; LbL; layer-by-layer; MUA; 11-mercaptoundecanoic
Sandwich-type electrochemical immunosensor for CEA detection based on Ag/MoS2@Fe3O4 and an analogous ELISA method with total internal reflection microscopy
Keywords: کل فلورسنس بازتاب داخلی; Electrochemical immunosensor; Carcinoembryonic antigen; ELISA; Total internal reflection fluorescence; Ag/MoS2@Fe3O4;
VAMP8, a vesicle-SNARE required for RAB37-mediated exocytosis, possesses a tumor metastasis suppressor function
Keywords: کل فلورسنس بازتاب داخلی; RAB37; VAMP8; TIMP1; Exocytosis; Metastasis; Prognosis; CM; conditional medium; IF; immunofluorescence; IHC; immunohistochemistry; IP; immunoprecipitation; SNAREs; soluble N-ethylmaleimide-sensitive factor activating protein receptors; TIMP1; tissue inhib
Degradation of fibrin-β amyloid co-aggregate: A novel function attributed to ubiquitin
Keywords: کل فلورسنس بازتاب داخلی; AD; Alzheimer disease; AFM; atomic force microscopy; CAA; cerebral amyloid angiopathy; CHCA; α-cyano-4-hydroxycinnamic acid; DAPI; 4â²,6-diamidino-2-phenylindole; DMEM; Dulbecco's modified eagle medium; DMSO; dimethyl sulfoxide; ELISA; enzyme-linked imm
A DEAD-Box Helicase Mediates an RNA Structural Transition in the HIV-1 Rev Response Element
Keywords: کل فلورسنس بازتاب داخلی; Rev; regulator of expression of virion; RRE; Rev response element; DDX1; DEAD-box protein 1; NIS; nuclear diffusion inhibitory signal; EMSA; electrophoretic mobility shift assay; MBP; maltose binding protein; TIRF; total internal reflection fluorescence;
Rab7a regulates cell migration through Rac1 and vimentin
Keywords: کل فلورسنس بازتاب داخلی; HA; Hemagglutinin; EGFP; Enhanced green fluorescent protein; GST; Glutathione S-transferase; TIRF; Total internal reflection fluorescence; Rab7a; Cell migration; Vimentin; Rac1; Myosin X; β1-integrin;
Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques
Keywords: کل فلورسنس بازتاب داخلی; ABCG2; Oligomerisation; Fluorescence correlation spectroscopy; Photon counting histogram; Total internal reflection fluorescence; Stepwise photobleaching;
Regions outside of conserved PxxPxR motifs drive the high affinity interaction of GRB2 with SH3 domain ligands
Keywords: کل فلورسنس بازتاب داخلی; DLS; dynamic light scattering; ITC; isothermal titration calorimetry; PRR; proline rich region; TIRF; total internal reflection fluorescence; SH3 domains; GRB2; SOS1; CBL; Signal transduction;
PALM and STORM: Into large fields and high-throughput microscopy with sCMOS detectors
Keywords: کل فلورسنس بازتاب داخلی; SR; super-resolution; SMLM; Single-Molecule Localization Microscopy; sCMOS; scientific Complementary Metal-Oxide Semiconductor; EMCCD; Electron-Multiplying Charge Coupled Device; SNR; signal-to-noise ratio; FOV; field of view; TIRF; Total Internal Reflect
N-Linked glycan site occupancy impacts the distribution of a potassium channel in the cell body and outgrowths of neuronal-derived cells
Keywords: کل فلورسنس بازتاب داخلی; Kv channel; voltage-gating potassium channel; CB; cell body; OG; outgrowth; CDG; congenital disorder of glycosylation; TIRF; total internal reflection fluorescence; DIC; differential interference contrast; Neu; neuraminidase; N-glycosylation; Potassium ch
Real-time imaging of exocytotic mucin release and swelling in Calu-3 cells using acridine orange
Keywords: کل فلورسنس بازتاب داخلی; Acridine orange; Spectral shift; Fluorescence lifetime; Total internal reflection fluorescence; Mucin granules; Cystic fibrosis;
Chemomechanical coupling mechanism of F1-ATPase: Catalysis and torque generation
Keywords: کل فلورسنس بازتاب داخلی; ÎG; change in free energy; KdATP; dissociation constant of ATP; ÎG2(θ); energy difference between transition state and post-reaction state; ÎG1(θ); energy difference between transition state and pre-reaction state; KEhyd; equilibrium constant of ATP
TM5275 prolongs secreted tissue plasminogen activator retention and enhances fibrinolysis on vascular endothelial cells
Keywords: کل فلورسنس بازتاب داخلی; tPA; tissue plasminogen activator; PAI-1; plasminogen activator inhibitor-1; VEC; vascular endothelial cell; TIRF; total internal reflection fluorescence; HBS; HEPES-buffered solution; CLSM; confocal laser scanning microscope; fibrinolysis; plasminogen ac
CaV1.2 sparklets in heart and vascular smooth muscle
Keywords: کل فلورسنس بازتاب داخلی; Coupled gating; Hypertension; Voltage-gated calcium channels; Total internal reflection fluorescence;
Selective accumulation and growth inhibition of hybrid liposomes to human hepatocellular carcinoma cells in relation to fluidity of plasma membranes
Keywords: کل فلورسنس بازتاب داخلی; BCNU; 3-bis(2-chloroethyl)-1-nitrosourea; C12(EO)23; polyoxyethylene(23) dodecyl ether; DDS; drug delivery system; DMPC; l-α-dimyristoylphosphatidylcholine; DPH; 1,6-diphenyl-1,3,5-hexatriene; HCC; hepatocellular carcinoma; HLs; hybrid liposomes composed
Different subcellular populations of L-type Ca2+ channels exhibit unique regulation and functional roles in cardiomyocytes
Keywords: کل فلورسنس بازتاب داخلی; LTCC; L-type Ca2+ channel; EC; excitation-contraction; ICa,L; L-type Ca2+ current; SR; sarcoplasmic reticulum; T-tubules; transverse tubules; CICR; Ca2+-induced Ca2+ release; [Ca2+]i; free intracellular Ca2+ concentration; PKA; protein kinase A; Cav; ca
DPP-4 inhibitor des-F-sitagliptin treatment increased insulin exocytosis from db/db mice β cells
Keywords: کل فلورسنس بازتاب داخلی; DPP-4; dipeptidyl peptidase-IV; CCD; charge-coupled device; ELISA; enzyme-linked immunosorbent assay; GFP; green fluorescent protein; KRB; Krebs ringer buffer; TIRF; total internal reflection fluorescence; TIRFM; TIRF microscopy; t-SNARE; target membrane
Plant actin controls membrane permeability
Keywords: کل فلورسنس بازتاب داخلی; nsPEFs; nanosecond pulsed electric fields; TIRF; total internal reflection fluorescence; Actin; Plant cell; Pulsed electric field; Tobacco BY-2; Total internal reflection microscopy;
Influence of PEGylation with linear and branched PEG chains on the adsorption of glucagon to hydrophobic surfaces
Keywords: کل فلورسنس بازتاب داخلی; PEGylation; Protein adsorption; Glucagon; Isothermal titration calorimetry; Fixed-angle optical reflectometry; Total internal reflection fluorescence
Optimization of antibody-conjugated magnetic nanoparticles for target preconcentration and immunoassays
Keywords: کل فلورسنس بازتاب داخلی; Immunoassay; Magnetic nanoparticles; Total internal reflection fluorescence; Array Biosensor; Protein microarrays
New insight into the Nox4 subcellular localization in HEK293 cells: First monoclonal antibodies against Nox4
Keywords: کل فلورسنس بازتاب داخلی; NADPH oxidase 4; Monoclonal antibodies; Subcellular localization; O2â; IPTG; Isopropyl β-d-1-thiogalactopyranoside; ER; endoplasmic reticulum; ROS; reactive oxygen species; DFP; Diisopropylfluoro-phosphate; eGFP; enhanced Green Fluorescent Protein; SOD
Ratiometric FRET-based detection of DNA and micro-RNA on the surface using TIRF detection
Keywords: کل فلورسنس بازتاب داخلی; Ratiometric fluorescence assay; Förster resonance energy transfer; FRET; Total internal reflection fluorescence; DNA hybridization; DNA and RNA detection;
Pattern of rise in subplasma membrane Ca2+ concentration determines type of fusing insulin granules in pancreatic β cells
Keywords: کل فلورسنس بازتاب داخلی; GFP; green fluorescent protein; HCSP; highly Ca2+ sensitive pool; IRP; immediately releasable pool; KRB; Krebs Ringer Buffer; TIRF; total internal reflection fluorescence; Exocytosis; Insulin granule; Pancreatic β cell; Total internal reflection fluoresc
Adsorption of a protein–porphyrin complex at a liquid–liquid interface studied by total internal reflection synchronous fluorescence spectroscopy
Keywords: کل فلورسنس بازتاب داخلی; Liquid–liquid interface; Total internal reflection fluorescence; Synchronous fluorescence; Porphyrin; Protein
Alzheimer's disease protein Aβ1–42 does not disrupt isolated synaptic vesicles
Keywords: کل فلورسنس بازتاب داخلی; Amyloid beta; Synaptic vesicle; Alzheimer's disease; Total internal reflection fluorescence; Integrity; Leakage
Selective Protein Immobilization via DNA Conjugation under Microfluidic Laminar Flow
Keywords: کل فلورسنس بازتاب داخلی; Microfluidic device; Protein sensor; DNA-directed immobilization; Total internal reflection fluorescence;
Munc18a: Munc-y business in mediating exocytosis
Keywords: کل فلورسنس بازتاب داخلی; APP; amyloid precursor protein; Cdk5; cyclin-dependent kinase 5; DOC2; double C2-domain; FRET; fluorescence resonance energy transfer; LDCV; large dense core vesicle; Mint; Munc18-interacting protein; PKC; protein kinase C; SM protein; Sec1/Munc18; SNARE;
The effect of substrate and adsorbed proteins on adhesion, growth and shape of CaCo-2 cells
Keywords: کل فلورسنس بازتاب داخلی; ECM proteins; Adhesion; Proliferation; Cell shape; ECM; extracellular matrix; PS; polystyrene; ATTC; American type culture collection; EHS; Engelbreth-Holm-Swarm; FBS; fetal bovine serum; HBSS; Hank's balanced salt solution; BrdU; 5-bromo-2-deoxyuridi
A label-free continuous total-internal-reflection-fluorescence-based immunosensor
Keywords: کل فلورسنس بازتاب داخلی; Biosensor; Carbohydrate; Evanescent wave; Fluorescence spectroscopy; Hapten; Immunosensor; Monoclonal antibody; Optical biosensor; Protein–carbohydrate interactions; Total internal reflection fluorescence; Transient binding; Weak affinity
From micro to nano: recent advances in high-resolution microscopy
Keywords: کل فلورسنس بازتاب داخلی; CCD; charge-coupled device; HELM; harmonic excitation light microscopy; MPM; multiphoton microscopy; NSOM; near-field scanning optical microscopy; PSF; point spread function; SHG; second harmonic generation; SPIM; selective plane illumination microscopy;
G protein threshold behavior in the human neutrophil oxidant response: measurement of G proteins available for signaling in responding and nonresponding subpopulations
Keywords: کل فلورسنس بازتاب داخلی; PT; pertussis toxin; CHO-MLF; N-formyl-methionyl-leucyl-phenylalanine; GAS; number of G proteins available for signaling per cell; DHR-123; dihydrorhodamine-123; ECL+; enhanced chemiluminescence plus; MGB; modified Gey's buffer; TIRF; total internal refle
New methodologies for measuring protein interactions in vivo and in vitro
Keywords: کل فلورسنس بازتاب داخلی; AFM; atomic force microscopy/microscope; BRET; bioluminescence resonance energy transfer; DBD; DNA-binding domain; FRET; fluorescence resonance energy transfer; GFP; green fluorescent protein; GPCR; G-protein-coupled receptor; PCA; protein fragment comple