کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765842 | 1050607 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pattern of rise in subplasma membrane Ca2+ concentration determines type of fusing insulin granules in pancreatic β cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
We simultaneously analyzed insulin granule fusion with insulin fused to green fluorescent protein and the subplasma membrane Ca2+ concentration ([Ca2+]PM) with the Ca2+ indicator Fura Red in rat β cells by dual-color total internal reflection fluorescence microscopy. We found that rapid and marked elevation in [Ca2+]PM caused insulin granule fusion mostly from previously docked granules during the high KCl-evoked release and high glucose-evoked first phase release. In contrast, the slow and sustained elevation in [Ca2+]PM induced fusion from newcomers translocated from the internal pool during the low KCl-evoked release and glucose-evoked second phase release. These data suggest that the pattern of the [Ca2+]PM rise directly determines the types of fusing granules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 385, Issue 3, 31 July 2009, Pages 291-295
Journal: Biochemical and Biophysical Research Communications - Volume 385, Issue 3, 31 July 2009, Pages 291-295
نویسندگان
Mica Ohara-Imaizumi, Kyota Aoyagi, Yoko Nakamichi, Chiyono Nishiwaki, Takashi Sakurai, Shinya Nagamatsu,