کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10140893 | 1646046 | 2019 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Applicability of biological dye tracer in strip biosensor for ultrasensitive detection of pathogenic bacteria
ترجمه فارسی عنوان
استفاده از ردیاب رنگی بیولوژیکی در نوار بیوسنسور برای تشخیص حساسیت بالای باکتری های بیماری زا
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Here, a facile, label-free and sensitive lateral flow strip (LFS) biosensor for foodborne pathogens was established relying on the innovative introduction of Gram staining and the direct immunoreaction. Target bacteria can be directly marked with crystal violet (CV) by one-step staining which is superior to traditional signal marking techniques in LFS assay, and the method's selectivity can be guaranteed by high-specificity monoclonal antibody. With Salmonella Enteritidis (S. Enteritidis) as a model target, this protocol can selectively detect 80â¯CFUâ¯mLâ1S. Enteritidis within 11â¯min in the optimized conditions. Moreover, with Listeria monocytogenes as another model target, the biosensor shows a high universality for detections of both gram-negative and gram-positive bacteria. The unexpected applicability of biological dye tracer in strip biosensor reveals that the biological dye can be a potential tool serving as a universal signal tracer for pathogenic microorganisms in food safety monitoring and early clinical diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 816-821
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 816-821
نویسندگان
Tong Bu, Qiong Huang, Lingzhi Yan, Wentao Zhang, Leina Dou, Lunjie Huang, Qingfeng Yang, Bingxin Zhao, Baowei Yang, Tao Li, Jianlong Wang, Daohong Zhang,