کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160531 | 139 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement in ligninolytic activity of Phanerochaete chrysosporium cultures by glucose oxidase
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effects of glucose oxidase (GOx) addition on enzymatic activity of manganese peroxidase (MnP) and lignin peroxidase (LiP) and protein production of Phanerochaete chrysosporium cultures were studied for the first time. In cultures supplemented daily with 300Â U/L GOx after beginning of ligninolysis, MnP activity was increased 4-fold (6144Â U/L) on day 15, LiP activity was induced (maximum activity was 220Â U/L.) and protein content of culture filtrates was increased significantly as compared to the control culture (i.e., no GOx-glucose was added.). In addition, remarkable increase in stability of MnP productivity by fungi, in cultures supplemented daily with GOx-glucose (more than 32 days), as compared with control cultures (22 days) was observed. Neither H2O2 nor gluconic acid produced by GOx was responsible for improvement of peroxidase activity in nitrogen-limited cultures. The new findings could be useful for improving the yield of peroxidase enzymes for degradation of a broad range of man-made aromatic compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 105, Part B, 15 January 2016, Pages 332-338
Journal: Biochemical Engineering Journal - Volume 105, Part B, 15 January 2016, Pages 332-338
نویسندگان
Zahra Ansari, Afzal Karimi, Sirous Ebrahimi, Elham Emami,