کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162200 | 1114320 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative Measurement of Toll-like Receptor 4 Agonists Adsorbed to Alhydrogel® by Fourier Transform Infrared-Attenuated Total Reflectance Spectroscopy
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Aluminum salts have a long history as safe and effective vaccine adjuvants. In addition, aluminum salts have high adsorptive capacities for vaccine antigens and adjuvant molecules, for example, Toll-like receptor 4 (TLR4) agonists. However, the physicochemical properties of aluminum salts make direct quantitation of adsorbed molecules challenging. Typical methods for quantifying adsorbed molecules require advanced instrumentation, extreme sample processing, often destroy the sample, or rely on an indirect measurement. A simple, direct, and quantitative method for analysis of adsorbed adjuvant molecules is needed. This report presents a method utilizing Fourier transform infrared spectroscopy with a ZnSe-attenuated total reflectance attachment to directly measure low levels (< 30 μg/mL) of TLR4 agonists adsorbed on aluminum salts with minimal sample preparation. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 104:768-774, 2015
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 2, February 2015, Pages 768-774
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 2, February 2015, Pages 768-774
نویسندگان
Quinton M. Dowling, Alicia M. Schwartz, Thomas S. Vedvick, Christopher B. Fox, Ryan M. Kramer,