کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162206 | 1114323 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In Vivo Analysis of the Potency of Silicone Oil Microdroplets as Immunological Adjuvants in Protein Formulations
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کلمات کلیدی
Adjuvant effect - اثر adjuvantParticle size - اندازه ذراتImmunology - ایمونولوژیprotein aggregation - تجمع پروتئینSubvisible particles - ذرات ذاتیsilicone oil - روغن سیلیکونProtein structure - ساختار پروتئینPrefilled syringes - سرنگ پر شدهProtein formulation - فرمول پروتئینImmune response - پاسخ یا واکنش ایمنی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
Subvisible particles in a therapeutic protein product may act as adjuvants to promote unwanted immune responses against the protein. Silicone oil is used as a lubricant in prefilled syringes, and microdroplets of silicone oil are often detected in protein formulations expelled from prefilled syringes. In order to test the adjuvant potency of silicone oil microdroplets, antibody responses in mice to subcutaneous injections of formulations of ovalbumin (OVA) that contained silicone oil microdroplets were measured. These responses were compared against responses to oil-free OVA formulations and to OVA formulations that contained microparticulate aluminum hydroxide (“alum”), the common vaccine adjuvant. When administered with high concentrations of silicone oil microdroplets, OVA formulations elicited strong anti-OVA IgG1 and IgG2a antibody responses. These responses were equivalent to those observed when alum microparticles were added to OVA formulations, suggesting that silicone oil can act as a potent adjuvant. However, when OVA formulations were prepared with lower levels of silicone oil that had been obtained directly from commercial siliconized syringes, the anti-OVA antibody response was not enhanced significantly compared with responses against OVA alone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 11, November 2015, Pages 3681-3690
Journal: Journal of Pharmaceutical Sciences - Volume 104, Issue 11, November 2015, Pages 3681-3690
نویسندگان
Carly Fleagle Chisholm, Bao Han Nguyen, Kaitlin R. Soucie, Raul M. Torres, John F. Carpenter, Theodore W. Randolph,