کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162333 | 1114326 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polylactide-Based Paclitaxel-Loaded Nanoparticles Fabricated by Dispersion Polymerization: Characterization, Evaluation in Cancer Cell Lines, and Preliminary Biodistribution Studies
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کلمات کلیدی
PLADrug release - انتشار مواد مخدرCytotoxicity - سمیت سلولیPolymer synthesis - سنتز پلیمرMacromonomer - ماکروومونومرIntracellular localization - محلی سازی داخل سلولیBiodegradable polymers - پلیمر زیست تخریب پذیرDispersion polymerization - پلیمریزاسیون پراکندگیCell culture - کشت سلولControlled release/delivery - کنترل انتشار / تحویل
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
The macromonomer method was used to prepare cross-linked, paclitaxel-loaded polylactide (PLA)-polyethylene glycol (stealth) nanoparticles using free-radical dispersion polymerization. The method can facilitate the attachment of other molecules to the nanoparticle surface to make it multifunctional. Proton nuclear magnetic resonance and Fourier transform infrared spectra confirm the synthesis of PLA macromonomer and cross-linking agent. The formation of stealth nanoparticles was confirmed by scanning and transmission electron microscopy. The drug release isotherm of paclitaxel-loaded nanoparticles shows that the encapsulated drug is released over 7 days. In vitro cytotoxicity assay in selected breast and ovarian cancer cell lines reveal that the blank nanoparticle is biocompatible compared with medium-only treated controls. In addition, the paclitaxel-loaded nanoparticles exhibit similar cytotoxicity compared with paclitaxel in solution. Confocal microscopy reveals that the nanoparticles are internalized by MCF-7 breast cancer cells within 1Â h. Preliminary biodistribution studies also show nanoparticle accumulation in tumor xenograft model. The nanoparticles are suitable for the controlled delivery of bioactive agents. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2546-2555, 2014
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 8, August 2014, Pages 2546-2555
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 8, August 2014, Pages 2546-2555
نویسندگان
Simeon K. Adesina, Alesia Holly, Gabriela Kramer-Marek, Jacek Capala, Emmanuel O. Akala,