|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|101638||161287||2016||6 صفحه PDF||سفارش دهید||دانلود کنید|
• A method capable of quantifying endogenous GHB level in hair sample was developed and validated.
• The mean measured concentration of GHB level for 0–3 cm hair was 1.93 ± 1.40 ng/mg (n = 66).
• The endogenous GHB level in the hair of males was significantly higher than the level of females.
• Due to the wide distribution of endogenous GHB, it is hard to make a reasonable “cut off” in hair.
Endogenous production complicates interpretation when gamma-hydroxybutyrate (GHB) is measured in hair for forensic purposes. A method capable of quantifying the endogenous concentration of GHB in human head hair was developed and validated using GC/MS/MS. Hair was digested under alkaline conditions (1 mol/L NaOH, 90 °C 10 min), and GHB-d6 was used as an internal standard. Before derivatization with BSTFA and ethyl acetate, a liquid–liquid extraction with ethyl acetate under acidic conditions was performed. GHB-TMS derivatives were detected using GC/MS/MS in the multiple-reaction monitoring mode. This method exhibited good linearity (y = 0.018x + 0.038, R2 = 0.9998), and the limit of detection was 0.02 ng/mg. The extraction recoveries were more than 60%, and the inter-day and intra-day relative standard deviations (RSD) were less than 15%. This method has been applied for the analysis of the endogenous GHB in hair samples from 66 drug-free Chinese donors. The mean measured concentration for 0–3 cm hair was 1.93 ± 1.40 ng/mg (n = 66), and extreme values were in the range of 0.28–4.91 ng/mg. The mean male endogenous GHB level was 2.95 ng/mg (0.92–4.91 ng/mg, n = 35), while the mean female level was 0.77 ng/mg (0.28–1.95 ng/mg, n = 31). This method was applied to a forensic case for the determination of GHB in hair samples but it is hard to make a reasonable “cut off” in hair. The solution is to use each subject as his own control.
Journal: Journal of Forensic and Legal Medicine - Volume 39, April 2016, Pages 10–15