کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
101720 161290 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Postmortem diagnosis of cytomegalovirus and accompanying other infection agents by real-time PCR in cases of sudden unexpected death in infancy (SUDI)
ترجمه فارسی عنوان
تشخیص پس از قاعدگی از سیتومگالوویروس و همراه شدن سایر عوامل عفونت توسط PCR در زمان واقعی در موارد مرگ ناگهانی غیرمنتظره در دوران بارداری (SUDI)
کلمات کلیدی
کالبد شکافی سیتومگالوویروس؛ SUDI؛ پارافین بافته شده؛ میکروبیولوژی پس از مرگ؛ PCR در زمان واقعی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی


• CMV may lead to fatal disseminated CMV infection of the premature and newborn.
• Our study is the first comprehensive study investigating CMV infections in SUDI.
• CMV suppresses the immune system, and may predispose to other infections.
• Postmortem molecular investigations are useful in explaining cause of death in SUDI.

As an opportunistic pathogen with high mortality rates, Cytomegalovirus (CMV) may lead to fatal disseminated CMV infection of the premature and newborn; thus necessitating the demonstration of CMV-DNA with clinical history and/or histopathological findings of CMV infection and defining other bacterial and viral infection agents with real-time polymerase chain reaction (RT-PCR) in udden unexpected death in infancy (SUDI) cases as we aimed in this study.314 (144 female, 170 male) SUDI cases were prospectively investigated from January 2013 to January 2015 in Istanbul Forensic Medicine Institution. The study includes 87 tissue samples of 39 cases for post-mortem histopathological examination of interstitial pneumonia, myocarditis, meningitis, encephalitis, hepatitis, colitis or tubulointerstitial nephritis and/or accompanying chronic sialadenitis.CMV-DNA was found positive in 35 (40.2%) salivary gland, 19 (21.8%) lung, 1 (1.1%) tonsil, and 1 (1.1%) brain tissues. CMV sialadenitis and/or CMV pneumonia associated with other viral and/or bacterial agents were detected in 23 (60%) of 39 infant cases.The demonstration of CMV-DNA would significantly clarify the cause of death and collection of epidemiological data in SUDI cases with clinical history and histopathological findings of CMV infection accompanying chronic CMV sialadenitis. Furthermore, CMV suppresses the immune system, and may predispose to other bacterial and/or viral infections in these cases. Post-mortem molecular investigations are useful in explaining cause of death in SUDI with a suspicion of infection in forensic autopsies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Forensic and Legal Medicine - Volume 38, February 2016, Pages 18–23
نویسندگان
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