Evaluating the effects of coal gasification slag on the fate of antibiotic resistant genes and mobile genetic elements during anaerobic digestion of swine manure

Coal gasification slag (GS) is an industrial solid waste with a highly developed pore structure, which can be used in anaerobic digestion (AD) to remove antibiotic resistance genes (ARGs) due to its structure, thereby utilizing this waste resource. This study evaluated the effects of three GS levels (0, 5, and 10 g/L) on the abundances of ARGs, mobile genetic elements, and the bacterial community. With GS added at 10 g/L, the removal rates for ARGs (dfrA7, sul2, tetW, ermF, and ermQ) were 24.81-90.48% after AD, and the removal rate for ISCR1 was 95.4%. In addition, 10 g/L GS was more effective at reducing the abundances of potential human pathogens. The variations in ARGs may have been affected by the succession of the microbial community. The results of this study demonstrate that supplementation with 10 g/L GS is more useful for reducing ARGs during AD.