کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233119 | 42679 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Medium optimization and immobilization of purified fibrinolytic URAK from Bacillus cereus NK1 on PHB nanoparticles
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Blood clot degrading enzymes are very much useful in combating cardiovascular diseases. An organism primarily isolated from soil, identified as Bacillus cereus NK1 was found to secrete an extracellular protease and the enzyme was identified to degrade fibrin. Response surface methodology was applied to optimize the URAK production medium by B. cereus NK1. The optimized medium obtained from central composite rotary design (CCRD) composed of glucose: 0.5%; soybean meal: 0.5%: calcium chloride: 0.5% and MgSO4: 0.2%. The production of URAK by the optimized medium was 6326.98 FU/ml. Initial experiments showed that the maximum production of URAK occurred at 12th h at pH 9. The efficacy of the medium was further studied in a fermentor as batch fermentation with the same medium optimized by RSM and the yield was comparable with that of the shake flask culture. The maximum activity obtained from fermentor was 6266.66 FU/ml, at the 12th h. PHB was synthesized by B. cereus DV-4 and used for the synthesis of PHB nanoparticles. URAK was purified by ion exchange chromatography and immobilized on PHB nanoparticles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 47, Issue 6, 8 November 2010, Pages 297-304
Journal: Enzyme and Microbial Technology - Volume 47, Issue 6, 8 November 2010, Pages 297-304
نویسندگان
Venkataraman Deepak, Shailaja Ilangovan, Madhumitha Vangal Sampathkumar, Maria Jacintha Victoria, Sheik Pran Babu Sardar Pasha, Suresh Babu Ram Kumar Pandian, Sangiliyandi Gurunathan,