کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233184 | 42733 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of tomato pectinase on Con A-Seralose 4B by bioaffinity layering
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Tomato pectinase was immobilized on agarose linked Concanavalin A by successive layering of the lectin and the enzyme. After four layers of the enzyme, the optimum effectiveness factor of 0.73 was obtained. The immobilized enzyme showed slightly higher Vmax/Km (0.4) as compared to free enzyme (0.3). The immobilized enzyme showed enhanced thermal stability at 50 °C, enhanced storage stability at 4 °C and enhanced stability during exposure to 4 M urea. Higher conversion of polygalacturonic acid at 50 °C by the immobilized enzyme as compared to the free enzyme was observed. The immobilized enzyme could be reused three times without any loss in activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 3, 2 August 2005, Pages 355-359
Journal: Enzyme and Microbial Technology - Volume 37, Issue 3, 2 August 2005, Pages 355-359
نویسندگان
Meryam Sardar, Munishwar N. Gupta,