کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233566 | 42757 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recovery of biological activity in reversibly inactivated proteins by three phase partitioning
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Recovery of biological activity in reversibly inactivated proteins by three phase partitioning Recovery of biological activity in reversibly inactivated proteins by three phase partitioning](/preview/png/10233566.png)
چکیده انگلیسی
Three phase partitioning (TPP) is carried out by adding ammonium sulphate and t-butanol to an aqueous solution of proteins. Under optimized conditions, the protein appears as an interfacial precipitate between upper t-butanol and lower aqueous phases. It is shown that TPP can be used to recover biological activities of cellulase, cellobiase and β-glucosidase from their urea-denatured forms. The activities were originally present in a commercial preparation and TPP led to simultaneous purification as well. The best results were 94% regain of activity with 73-fold purification for cellulase, 98% regain of activity with 65-fold purification for cellobiase and 90% regain of activity with 101-fold purification for β-glucosidase. Working with more concentrated protein solutions was possible but poor results were obtained beyond 10 mg mlâ1 of the starting protein concentration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 1, 1 June 2005, Pages 113-120
Journal: Enzyme and Microbial Technology - Volume 37, Issue 1, 1 June 2005, Pages 113-120
نویسندگان
Ipsita Roy, Aparna Sharma, Munishwar Nath Gupta,